异养硝化菌Acinetobacter sp.的分离鉴定及其脱氮特性

发布时间：2018-09-17 11:58

【摘要】：从北京市高碑店污水处理厂BBR(Bacillus Bacteria Bioreactor)中试设备的活性污泥样品中分离得到一株异养硝化-好氧反硝化菌株JQ1004,利用16S r DNA对其进行同源性分析,初步鉴定其为不动杆菌属(Acinetobacter sp.).在好氧条件下,以丁二酸钠为碳源,分别以氨氮和硝酸盐氮为氮源,研究其脱氮特性,发现该菌株在33h时氨氮去除率达到99.45%,COD去除率达到92.54%;在34h时,硝酸盐氮去除率达到84.42%,而COD去除率达到93.11%.另外通过研究发现,与降解氨氮相比,菌株JQ1004在降解硝酸盐氮时需要更高的C/N.利用Compertz模型对其脱氮特性进行拟合发现,菌株对氨氮的最大降解速率明显高于降解硝酸盐氮的最大降解速率,分别为Rm=7.93mg/(L·h)和Rm=4.08mg/(L·h).利用响应面法进行实验设计,优化其脱氮条件,得到最佳脱氮条件为:pH=7.33;温度=31.80℃;转速=154.54r/min;C/N=7.76.
[Abstract]:A heterotrophic Nitrification-aerobic denitrification strain JQ1004 was isolated from the activated sludge samples of BBR (Bacillus Bacteria Bioreactor) pilot plant of Beijing Gaobeidian Sewage Treatment Plant. The strain was identified as Acinetobacter sp. Under aerobic conditions, sodium succinate was used as the catalyst. Carbon source, using ammonia nitrogen and nitrate nitrogen as nitrogen sources, the denitrification characteristics of the strain were studied. It was found that the removal rate of ammonia nitrogen and COD reached 99.45% and 92.54% respectively at 33H and 84.42% and 93.11% respectively at 34h. A higher C/N ratio is needed. The results show that the maximum degradation rate of ammonia nitrogen is obviously higher than that of nitrate nitrogen, Rm=7.93 mg/(L.h) and Rm=4.08 mg/(L.h). Response surface methodology is used to optimize the denitrification conditions. PH=7.33; temperature =31.80 C; speed =154.54r/min; C/N=7.76.
【作者单位】：北京工业大学建筑工程学院;
【基金】：水体污染控制与治理科技重大专项(2015ZX07202-013) 16人才培养质量建设-双培养计划新兴专业建设(004000542216031)
【分类号】：X172

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