锰污染土壤对酸模叶蓼氮素代谢的影响

发布时间：2018-10-19 11:45

【摘要】：探讨超富集植物高浓度金属富集量对其生理机制的影响,对于了解超富集植物强大的解毒机制与其生理机制的相关性有重要意义。采用室内原矿土栽培的方法,研究了贺州某锰矿区未开采区(T2)、探矿区(T3)、恢复区(T4)、开采区(T5)、尾矿库(T6)土壤及桂林未受污染的菜园土(T1)对锰超富集植物酸模叶蓼(Polygonum lapathifolium L.)生长,锰吸收,叶片铵态氮、硝态氮、游离脯氨酸、可溶性蛋白质含量及氮素代谢关键酶(硝酸还原酶NR)、谷氨酰胺合成酶GS、谷氨酸合酶GOGAT和谷氨酸脱氢酶GDH)活性的影响。结果表明,随着Mn处理浓度的提高,酸模叶蓼根、茎、叶中Mn含量显著增加(P0.05),叶绿素a、叶绿素b和总叶绿素在T3处理时含量最高,此时分别为对照的1.15、1.23和1.17倍,但所有锰处理下酸模叶蓼生物量无显著性差异(P0.05)。Mn处理一定程度上提高了叶片中可溶性蛋白质含量,引起了酸模叶蓼氮素代谢关键酶活性的变化及硝态氮、铵态氮和脯氨酸含量的显著增加(P0.05)。T6处理硝态氮、铵态氮和脯氨酸含量最高,分别比对照提高了17.6%、43.8%和206.2%。NR、GS和GOGAT活性呈先增加后降低的变化趋势,NR、GS和GOGAT活性分别在T2、T3、T4处理时最高,此时分别比对照提高了6.4%、28.8%和66.4%。GDH活性则随着Mn处理浓度的增加而增加,在T6处理时活性最高,比对照提高了1.74倍。以上表明,GDH在解毒Mn毒害过程中具有重要作用。
[Abstract]:It is important to explore the effect of high concentration metal enrichment on the physiological mechanism of superenriched plants, and to understand the correlation between the strong detoxification mechanism and physiological mechanism of super-enriched plants. The effects of unmined (T2), prospecting (T3), recovery (T4), mining area (T5), tailings (T6) and uncontaminated vegetable garden soil (T1) on manganese superenriched (Polygonum lapathifolium L.) were studied in a manganese mine area in Hezhou. Growth, manganese uptake, leaf ammonium nitrogen, nitrate nitrogen, free proline, soluble protein content and activities of key enzymes of nitrogen metabolism (nitrate reductase NR), glutamine synthase GS, glutamate synthase GOGAT and glutamate dehydrogenase GDH) were investigated. The results showed that the content of Mn in the root, stem and leaf of Polygonum hydropiper increased significantly with the increase of Mn concentration (P0.05). The content of chlorophyll a, chlorophyll b and total chlorophyll in the treatment of T3 was the highest, which was 1.150.23 and 1.17 times of that of the control, respectively. However, there was no significant difference in the biomass of Polygonum acuminatum under all manganese treatments (P0.05). Mn treatment increased the content of soluble protein in leaves to some extent, and caused the change of key enzyme activity of nitrogen metabolism and nitrate nitrogen in leaves of Polygonum hydropiper L.). The content of ammonium nitrogen and proline increased significantly (P0.05). Ammonium nitrogen and proline contents were the highest in T6 treatment, which increased by 17.6% and 206.2% respectively compared with the control. The activities of NR,GS and GOGAT increased first and then decreased. The activities of NR,GS and GOGAT were the highest in T2T3T4 treatment. Compared with the control, the activity of 66.4%.GDH and the activity of 66.4%.GDH were increased by 6.48% and 28.8%, respectively, and increased with the increase of Mn concentration. The activity of T6 was the highest and 1.74 times higher than that of the control. These results suggest that GDH plays an important role in the detoxification of Mn.
【作者单位】：邵阳学院城乡建设学院;广西师范大学生命科学学院;广西师范大学环境与资源学院;广西壮族自治区环境监测中心站;桂林电子科技大学生命与环境科学学院;
【基金】：国家自然科学基金项目(41661077;51368011) 国家重点研发计划项目(2017YFD0801500) 湖南省自然科学基金项目(2016JJ6135) 广西自然科学基金项目(2016GXNSFAA380046) 桂林市科学研究与技术开发项目(2016012504) 广西岩溶生态与环境变化研究重点实验室项目(YRHJ15K002;YRHJ15Z026)
【分类号】：X173;X53

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