厌氧氨氧化污泥群体感应信号分子检测及影响研究

发布时间：2018-11-15 21:52

【摘要】：厌氧氨氧化作为一种新型的脱氮工艺具有很好的应用前景,但厌氧氨氧化污泥富集培养困难.本研究以处理垃圾渗滤液的污泥作为接种污泥,在升流式厌氧污泥反应器(UASB)中启动厌氧氨氧化过程;检测驯化成功的厌氧氨氧化污泥中是否存在群体感应信号分子;并通过添加外源信号分子(cis-11-Methyl-2-dodecenoic acid,DSF和N-acyl-homoserine lactone,AHL),探究信号分子对厌氧氨氧化污泥附着生长能力的影响.结果表明,UASB反应器在运行150 d后,总氮去除率达到80%,菌群结构中厌氧氨氧化菌所占比例超过了20%,成功启动厌氧氨氧化工艺,且厌氧氨氧化污泥中存在低浓度的AHLs信号分子;人为添加3-碳位置取代基为羰基的AHLs(3-oxo-C6-HSL、3-oxo-C8-HSL、3-oxo-C10-HSL和3-oxo-C12-HSL)信号分子均能促进厌氧氨氧化污泥附着生长,而3-碳位置无取代基的AHLs中只有C12-HSL和C6-HSL存在积极影响,C8-HSL、C10-HSL和DSF信号分子对厌氧氨氧化污泥的附着生长能力并没有促进作用.
[Abstract]:As a new denitrification process, anaerobic ammonia oxidation has a good application prospect, but it is difficult to enrich and culture anaerobic ammonia oxidized sludge. In this study, the anaerobic ammonia oxidation process was initiated in the upflow anaerobic sludge reactor (UASB) by using the sludge treated with landfill leachate as the inoculation sludge, and whether there were group sensing signal molecules in the domesticated anaerobic ammonia oxidation sludge was detected. By adding exogenous signal molecules (cis-11-Methyl-2-dodecenoic acid,DSF and N-acyl-homoserine lactone,AHL), the effect of signal molecules on the ability of anaerobic ammonia oxidation sludge attachment was investigated. The results showed that the total nitrogen removal rate of the UASB reactor reached 80% after 150 days, and the proportion of anaerobic ammonia oxidizing bacteria in the microbial structure exceeded 20%. The anaerobic ammonia oxidation process was successfully started. And low concentration of AHLs signal molecule was found in anaerobic ammonia oxidized sludge. AHLs (3-oxo-C6-HSL-3-oxo-C8-HSL-3-oxo-C10-HSL and 3-oxo-C12-HSL) signaling molecules with 3-carbon position substituents as carbonyl groups can promote the growth of anaerobic ammoxidation sludge. However, only C12-HSL and C6-HSL have positive effects on AHLs with no substituents in 3-carbon position. C8-HSLC10-HSL and DSF signaling molecules have no effect on the adhesion and growth of anaerobic ammonia oxidized sludge.
【作者单位】：北京师范大学环境学院水沙科学教育部重点实验室水环境模拟国家重点实验室;深圳市水务(集团)有限公司;
【基金】：国家自然科学基金青年科学基金项目(51208038)
【分类号】：X703.1

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