水体中蓝藻水华分解产甲烷动态过程研究

发布时间：2018-11-21 18:26

【摘要】：在巢湖开展蓝藻分解的原位围隔实验,实验期间测定围隔中层水体溶解性甲烷浓度和主要理化因子,通过定量PCR的方法测定产甲烷菌mcrA基因拷贝数以及细菌和古菌16S rRNA基因拷贝数随时间的变化情况.结果显示:随着蓝藻有机质分解,水体产甲烷菌数量逐渐增加,溶解性甲烷浓度最高达到2.94mg/L;产甲烷菌mcrA基因拷贝数与甲烷浓度具有显著的正相关关系;同时还发现水体中的亚铁离子(Fe~(2+))含量与产甲烷菌的数量具有显著的正相关关系.上述结果支持蓝藻水华爆发导致水体也成为甲烷生物合成热点区域的观点.本研究获得的结果有助于进一步分析不同产甲烷热点区域(水体和沉积物)对湖泊甲烷总体释放量的贡献情况.
[Abstract]:In situ experiments on the decomposition of cyanobacteria were carried out in Chaohu Lake. During the experiment, the concentration of dissolved methane and the main physical and chemical factors were measured. The mcrA gene copy number of methanogenic bacteria and the variation of 16s rRNA gene copy number of bacteria and archaea with time were measured by quantitative PCR. The results showed that with the decomposition of organic matter of cyanobacteria, the amount of methanogenic bacteria in water increased gradually, and the highest concentration of dissolved methane reached 2.94 mg / L, and the copy number of mcrA gene of methanogenic bacteria was positively correlated with the concentration of methane. It was also found that the content of Fe~ (2) in water was positively correlated with the quantity of methanogenic bacteria. These results support the view that the eruption of cyanobacteria Shui Hua leads to the water body becoming a hot spot of methane biosynthesis. The results obtained in this study are helpful to further analyze the contribution of different hot spots (water and sediment) of methane production to the total release of methane from lakes.
【作者单位】：南京师范大学生命科学学院;中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室;中国科学院大学;南京信息工程大学应用气象学院;
【基金】：国家“863”项目(2014AA06A509) 国家自然科学基金资助目(31370508) 中国科学院青年创新促进会(2014273) 湖泊与环境国家重点实验室开放研究基金(2012SKL005)
【分类号】：X524

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