水体微生物污染指示菌在不同营养水平下的稳定性

发布时间：2018-12-11 01:16

【摘要】：微生物污染指示菌在水体中的稳定性直接影响到定量的准确性及应用的适应性.通过建立水环境模拟反应器,以实时荧光定量PCR方法探究鸡粪中大肠埃希氏菌(EC)、拟杆菌(GB)及鸡源特异性基因(CB)等指示微生物在不同营养水平的水环境的稳定性及衰减特征.结果表明:所选微生物衰减较好的符合一级动力学模型.对照组和含有营养物质实验体系中指示微生物的衰减系数k分别为EC(0.135,0.134;前者为对照组,下同),GB(0.415,0.457),CB(0.425,0.437),说明营养物质对EC、GB、CB的衰减未有显著影响.有氧水环境中,GB、CB呈现快速衰减趋势,EC则变化相对缓慢.不同营养水平下3种微生物的衰减具有显著正相关性(P0.05),均可作为良好指示菌以评价水体的微生物污染程度,但特征各异.
[Abstract]:The stability of microbial contamination indicator bacteria in water directly affects the accuracy of quantitative analysis and the adaptability of application. The (EC), of Escherichia coli in chicken feces was studied by real-time fluorescence quantitative PCR method by establishing a water environment simulation reactor. Bacteroides (GB) and chicken specific gene (CB) were used to indicate the stability and attenuation of microbes in water environment with different nutrient levels. The results show that the selected microorganism decay is in good agreement with the first order kinetic model. The attenuation coefficient of indicated microorganisms in the control group and the experimental system containing nutrients was EC (0.135 卤0.134), respectively. The former was a control group, the other was), GB (0.415 ~ (0.457), CB (0.425 ~ (0.437), which indicated that there was no significant effect of nutrition on the attenuation of EC,GB,CB. In aerobic water environment, GB,CB showed a rapid attenuation trend, while EC changed relatively slowly. There was significant positive correlation between the attenuation of three microorganisms at different nutrient levels (P0.05), which could be used as a good indicator to evaluate the degree of microbial contamination in water, but the characteristics were different.
【作者单位】：武汉理工大学资源与环境工程学院;环境保护部华南环境科学研究所;华南理工大学生物科学与工程学院;广东省水与大气污染防治重点实验室;武汉科技大学资源与环境工程学院;
【基金】：国家自然科学基金项目(41303054) 中央级公益性科研院所基本科研业务专项
【分类号】：X172;X52

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