养殖污水中蛋白核小球藻的分离鉴定及其污水处理效果

发布时间：2018-12-17 01:55

【摘要】：利用BG-11培养基从养殖污水中分离和筛选到小球藻,采用相差显微镜对其外部形态进行观察、傅里叶红外光谱分析球藻组成、X-ray方法分析其晶体组成、凯氏定氮以及有机溶剂萃取方法分别测定其蛋白质以及脂质含量、丙酮萃取法测定其叶绿素a以及国标法测定其处理污水效果。结果表明:根据相差显微镜对球藻外部观察,初步判断该小球藻为蛋白核小球藻;红外光谱测定结果表明,在波数1 080和1 240 cm~(-1)处均有明显的吸收峰,证明了该研究分离的小球藻含有大量蛋白质以及糖类和脂类成分;X-衍射分析结果显示在32°和20°两处均有明显的吸收峰,证明了本研究分离的小球藻含有蛋白质和脂类成分;定量成分测定表明该蛋白核小球藻蛋白质量分数为45.6%,脂质质量分数为8.1%;污水处理结果为化学需氧量去除率为70.9%;总氮、总磷去除率分别为23%和34.7%。该研究对蛋白核小球藻蛋白含量、脂肪含量的测定以及提取进行了分析,证明了蛋白核小球藻对养殖场污水的处理具有很好的效果,为生物新资源的开发以及水环境治理提供了一定的参考。
[Abstract]:Chlorella microphylla was isolated and screened from culture wastewater by BG-11 medium. The external morphology was observed by phase contrast microscope. The composition of Chlorella was analyzed by Fourier transform infrared spectroscopy (FTIR). The crystal composition of Chlorella was analyzed by X-ray method. The contents of protein and lipid were determined by Kjeldahl nitrogen determination and organic solvent extraction method, chlorophyll a by acetone extraction method and the effect of sewage treatment by national standard method. The results showed that Chlorella sp. Was identified as Chlorella Proteinuca according to the external observation of Chlorella by phase contrast microscope. The infrared spectra showed that there were obvious absorption peaks at the wavenumber of 1080 and 1 240 cm~ (-1), which proved that the isolated Chlorella microphylla contained a large number of proteins, carbohydrates and lipids. The results of X-ray diffraction analysis showed that there were obvious absorption peaks at 32 掳and 20 掳, which proved that the isolated Chlorella microphylla contained protein and lipids. The quantitative component analysis showed that the protein fraction of Chlorella vulgaris was 45.6, the mass fraction of lipid was 8.1 and the removal rate of COD was 70.9. The removal rates of total nitrogen and total phosphorus were 23% and 34.7% respectively. The analysis of protein content, fat content and extraction of Chlorella protein showed that Chlorella Proteinuclear Chlorella had a good effect on the treatment of wastewater from the farm. It provides a certain reference for the development of new biological resources and water environment management.
【作者单位】：山东科技大学化学与环境工程学院;
【基金】：山东省自然科学基金面上项目(No.ZR2014EMM005) 山东省人力资源与社会保障厅资助项目(No.20101008) 潍坊市峡山水库管理局资助项目(No.201303201603)
【分类号】：X71

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