一种微生物脱氮菌剂的构建
发布时间：2018-12-17 05:27
【摘要】：随着工业、农业以及人们生活水平的提高,氮素污染已经成为全球性的重大环境问题,引起了社会各界的广泛关注,促使了生物脱氮技术的快速发展,新型生物脱氮技术也不断出现。其中异养硝化-好氧反硝化菌的出现使得硝化过程与反硝化过程得以在时间上和空间上实现统一,大大提高了脱氮的效率;微生物固定化技术的出现则克服了微生物不易保存运输,以及投加到后流失严重等问题。本研究从南昌市某污水处理厂二沉池污泥及南昌大学某湖泊底泥中筛选出了3株脱氮性能较好的异养硝化-好氧反硝化菌WN1、WN3和DN02,3株菌的氨氮去除率分别为68.9%、71.7%和66.2%。从混合培养初探实验结果看出三株菌可共生培养,通过混料设计得出了三株菌构成菌剂的最佳配比为WN1:WN3:DN02=0.935:0.965:1.100,在此最佳复合比下菌剂的氨氮去除率达到90.37%,NO_3~--N的最大浓度为7.58mg/L,NO2--N的浓度始终为0。进一步研究了菌剂的最佳脱氮条件,结果表明在碳源为琥珀酸钠,pH为7.0,温度为30℃,摇床转速为150rpm,C/N为12条件下菌剂的脱氮效果最好,氨氮去除率达到91.18%。当海藻酸钠为1%,聚乙烯醇为8%,活性炭为2%时,包埋固定化小球在稳定性,成球性和传质性能上都有较好的表现。固定化过程中使用的化学药剂会对微生物活性会产生一定的影响,但经过活性恢复实验后,固定化小球的脱氮性能与游离菌剂相当。当固定化小球投加量为20%时,氨氮去除率达到89.78%。固定化小球在模拟废水氨氮浓度低于500mg/L时,20h内氨氮去除率均能达到最大,且能维持在85%左右;当模拟废水氨氮浓度达到1000mg/L时,需要28h才能达到最大氨氮去除率81.7%。
[Abstract]:With the improvement of industry, agriculture and people's living standards, nitrogen pollution has become a major environmental problem in the world, which has aroused widespread concern from all walks of life and promoted the rapid development of biological nitrogen removal technology. New biological denitrification techniques are also emerging. The appearance of heterotrophic nitrification-aerobic denitrifying bacteria made the nitrification process and denitrification process unified in time and space and greatly improved the efficiency of nitrogen removal. The emergence of microorganism immobilization technology overcomes the problems such as the difficulty of preservation and transportation of microorganisms and the serious loss after addition. In this study, three strains of heterotrophic nitrification-aerobic denitrifying bacteria (WN1,WN3) and DN02,3 strains (WN1,WN3 and DN02,3) were screened from the sludge of secondary sedimentation tank and a lake sediment of Nanchang University. The ammonia nitrogen removal rates of WN1,WN3 and DN02,3 strains were 68.9, respectively. 71.7% and 66.2%. The results of mixed culture experiment showed that three strains of bacteria could be cultured symbiotically, and the optimum mixture ratio of three strains of bacteria was found to be WN1:WN3:DN02=0.935:0.965:1.100, by mixing design. Under the optimum compound ratio, the removal rate of ammonia-nitrogen reached 90.37% and the maximum concentration of N was 7.58 mg / L no _ 2--N, and the concentration of N was always 0.58 mg 路L ~ (-1) 路L ~ (-1) ~ (-1). The optimum denitrification conditions were further studied. The results showed that the best denitrification effect was obtained when the carbon source was sodium succinate, the pH was 7.0, the temperature was 30 鈩，
本文编号：2383725
[Abstract]:With the improvement of industry, agriculture and people's living standards, nitrogen pollution has become a major environmental problem in the world, which has aroused widespread concern from all walks of life and promoted the rapid development of biological nitrogen removal technology. New biological denitrification techniques are also emerging. The appearance of heterotrophic nitrification-aerobic denitrifying bacteria made the nitrification process and denitrification process unified in time and space and greatly improved the efficiency of nitrogen removal. The emergence of microorganism immobilization technology overcomes the problems such as the difficulty of preservation and transportation of microorganisms and the serious loss after addition. In this study, three strains of heterotrophic nitrification-aerobic denitrifying bacteria (WN1,WN3) and DN02,3 strains (WN1,WN3 and DN02,3) were screened from the sludge of secondary sedimentation tank and a lake sediment of Nanchang University. The ammonia nitrogen removal rates of WN1,WN3 and DN02,3 strains were 68.9, respectively. 71.7% and 66.2%. The results of mixed culture experiment showed that three strains of bacteria could be cultured symbiotically, and the optimum mixture ratio of three strains of bacteria was found to be WN1:WN3:DN02=0.935:0.965:1.100, by mixing design. Under the optimum compound ratio, the removal rate of ammonia-nitrogen reached 90.37% and the maximum concentration of N was 7.58 mg / L no _ 2--N, and the concentration of N was always 0.58 mg 路L ~ (-1) 路L ~ (-1) ~ (-1). The optimum denitrification conditions were further studied. The results showed that the best denitrification effect was obtained when the carbon source was sodium succinate, the pH was 7.0, the temperature was 30 鈩，
本文编号：2383725
本文链接：https://www.wllwen.com/shengtaihuanjingbaohulunwen/2383725.html
