一株双酚A降解菌的筛
发布时间：2019-01-07 12:13
【摘要】：双酚A(Bisphenol,简称BPA)是一种典型的内分泌干扰物,由于具有雌激素活性,人体摄入一定量这种物质后,会出现身体假早熟、胎儿和儿童发育不正常、人体内分泌失调等现象,甚至引发癌症。双酚A是一种人工合成的化学物质,它作为一种现代重要的化工原料被广泛应用于生活用品、轻工业等的方方面面,例如婴儿使用的奶瓶当中便含有双酚A。由于双酚A的制造或产品使用过程中存在泄露和废品的不恰当处理等现象,双酚A及其易污染水体、土壤和大气,进而通过食物链、食物网进入动物体和人体,经积累超过限值导致人体健康受到不良影响。由于双酚A的危害性,近年来其污染治理越来越受到人们的重视。传统方法中,物理、化学处理双酚A的过程有一定的局限性,导致处理结果有时达不到要求,因此生物降解的方法越来越受到重视,无论是在处理效果还是运行成本上都具有很大的优势及良好的发展前景。从沈阳北部污水处理厂曝气池的活性污泥中驯化、分离出25株具有双酚A降解能力的菌株,其中7株菌株的降解率均大于40%,分别命名为D-03、D-06、D-11、D-14、D-17、D-22和D-23。从这些降解能力较强的菌株中再次筛选得到了一株以双酚A为碳源的高效降解菌株D-17,并通过菌体形态、生理生化反应特性及16S r DNA基因测序分析对其进行初步鉴定。结果表明,菌株D-17为乙酸钙不动杆菌(Acinetobacter calcoaceticus)。菌株D-17的降解条件试验表明,该菌株的生长及降解双酚A的最适条件为溶液p H 7.0,接种量10%,摇床转速150 r/min,降解温度30℃,降解时间5 d,当双酚A初始浓度为60 mg/L,双酚A降解率可达52.62%。菌株D-17的最大耐受双酚A浓度是140 mg/L。另外,外加葡萄糖时,双酚A降解率降至40.37%;外加Na2HPO4+KH2PO4时,双酚A降解率增加到了60.01%;外加蛋白胨可使双酚A降解率提高至57.15%。
[Abstract]:Bisphenol A (BPA) is a typical endocrine disrupting substance. Because of its estrogenic activity, the body will become precocious, the fetus and children will not develop normally, and the human body will have endocrine disorders after ingesting a certain amount of this substance. It even causes cancer. Bisphenol A (BPA) is a synthetic chemical substance. As an important chemical material, bisphenol A is widely used in many aspects of daily necessities, light industry and so on. For example, bisphenol A is found in baby bottles. Due to the leakage of bisphenol A and improper disposal of waste products during the manufacture or use of bisphenol A, bisphenol A and its easily polluted water, soil and atmosphere enter animals and human bodies through the food chain and food web. Human health is adversely affected by accumulation exceeding the limit. Because of the harmfulness of bisphenol A, people pay more and more attention to its pollution control in recent years. In the traditional methods, the physical and chemical treatment of bisphenol A has some limitations, which leads to the treatment results sometimes not up to the requirements, so more and more attention has been paid to the biodegradation method. Both the processing effect and the running cost have great advantages and good development prospects. After acclimation from activated sludge in aeration tank of North Shenyang sewage treatment Plant, 25 strains with the ability to degrade bisphenol A were isolated. The degradation rates of 7 strains were all greater than 40. They were named D-03C D-06N D-11C D-14, respectively. D-17, D-22 and D-23. A highly efficient strain D-17 with bisphenol A as carbon source was obtained from these strains with strong biodegradation ability. The strain D-17 was preliminarily identified by cell morphology, physiological and biochemical reaction characteristics and 16s r DNA gene sequencing analysis. The results showed that strain D-17 was Acinetobacter calcium Acinetobacter (Acinetobacter calcoaceticus). The degradation conditions of strain D-17 showed that the optimum conditions for growth and degradation of bisphenol A were as follows: solution pH 7.0, inoculation amount 10, shaking speed 150 r / min, degradation temperature 30 鈩，
本文编号：2403652
[Abstract]:Bisphenol A (BPA) is a typical endocrine disrupting substance. Because of its estrogenic activity, the body will become precocious, the fetus and children will not develop normally, and the human body will have endocrine disorders after ingesting a certain amount of this substance. It even causes cancer. Bisphenol A (BPA) is a synthetic chemical substance. As an important chemical material, bisphenol A is widely used in many aspects of daily necessities, light industry and so on. For example, bisphenol A is found in baby bottles. Due to the leakage of bisphenol A and improper disposal of waste products during the manufacture or use of bisphenol A, bisphenol A and its easily polluted water, soil and atmosphere enter animals and human bodies through the food chain and food web. Human health is adversely affected by accumulation exceeding the limit. Because of the harmfulness of bisphenol A, people pay more and more attention to its pollution control in recent years. In the traditional methods, the physical and chemical treatment of bisphenol A has some limitations, which leads to the treatment results sometimes not up to the requirements, so more and more attention has been paid to the biodegradation method. Both the processing effect and the running cost have great advantages and good development prospects. After acclimation from activated sludge in aeration tank of North Shenyang sewage treatment Plant, 25 strains with the ability to degrade bisphenol A were isolated. The degradation rates of 7 strains were all greater than 40. They were named D-03C D-06N D-11C D-14, respectively. D-17, D-22 and D-23. A highly efficient strain D-17 with bisphenol A as carbon source was obtained from these strains with strong biodegradation ability. The strain D-17 was preliminarily identified by cell morphology, physiological and biochemical reaction characteristics and 16s r DNA gene sequencing analysis. The results showed that strain D-17 was Acinetobacter calcium Acinetobacter (Acinetobacter calcoaceticus). The degradation conditions of strain D-17 showed that the optimum conditions for growth and degradation of bisphenol A were as follows: solution pH 7.0, inoculation amount 10, shaking speed 150 r / min, degradation temperature 30 鈩，
本文编号：2403652
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