有机胺对螺旋藻生长及固碳效果的影响

发布时间：2019-03-27 21:10

【摘要】：采用微藻培养来降低空气中二氧化碳含量是当前研究的热点。然而,以气态CO_2为碳源的微藻培养过程中,普遍存在传质和固碳速率较低等问题。研究发现有机胺类物质如MEA(乙醇胺)、DEA(二乙醇胺)、MDEA(N-甲基-二乙醇胺)、TEA(三乙醇胺)等能够显著提高CO_2的气液传质速率。而针对有机胺类物质影响微藻培养及固碳效率的公开报道却并不多。研究以钝顶螺旋藻(FACHB-901)利用低含量CO_2气体为碳源的培养过程为对象,系统研究了有机胺类物质的种类(MDEA、MEA、DEA、TEA)及其添加策略对螺旋藻生物量和固碳速率的影响关系。研究结果表明,在改良的Zarrouk培养基中添加了1 mmol·L~(-1) MDEA可以使培养基中溶解性无机碳(DIC)含量达到297.4 mg·L~(-1)。而添加1mmol·L~(-1) TEA可以得到最佳的螺旋藻生物量(0.894 g·L~(-1))和固碳速率(139.3 mg·(L·d)~(-1))。同时,研究获得螺旋藻培养过程中TEA的最优添加策略为:延滞期添加5 mmol·L~(-1)、对数前期补加1 mmol·L~(-1)、对数后期补加2 mmol·L~(-1)。最终,通过TEA优化的添加策略获得了螺旋藻的生物量(1.248 g·L~(-1))和固碳速率(191.4 mg·(L·d)~(-1),比对照组分别提高了25.6%和41.2%。
[Abstract]:Microalgae culture to reduce the carbon dioxide content in the air is the focus of current research. However, in the process of microalgae culture with gaseous CO_2 as carbon source, the problems of mass transfer and low carbon sequestration rate are common. It was found that organic amines, such as MEA (), MDEA (N-methyl-diethanolamine), MDEA (N-diethanolamine), TEA (triethanolamine), could significantly increase the gas-liquid mass transfer rate of CO_2. However, there are few public reports on the effects of organic amines on microalgae culture and carbon sequestration efficiency. The culture process of Spirulina platensis (FACHB-901) using low content of CO_2 gas as carbon source was studied. The species of organic amines (MDEA,MEA,DEA,) were systematically studied. Effects of TEA) and its addition strategy on biomass and carbon sequestration rate of Spirulina platensis. The results showed that adding 1 mmol 路L ~ (- 1) MDEA to the modified Zarrouk medium could increase the soluble inorganic carbon (DIC) content to 297.4 mg 路L ~ (- 1). The optimum biomass (0.894 g 路L ~ (- 1) and carbon sequestration rate (139.3 mg (L 路d) ~ (- 1).) of Spirulina platensis could be obtained by adding 1mmol 路L ~ (- 1) TEA. At the same time, the optimal strategy of adding TEA in the culture process of Spirulina platensis was as follows: 5 mmol 路L ~ (- 1) in lag period, 1 mmol 路L ~ (- 1) in logarithmic pre-phase and 2 mmol 路L ~ (- 1) in logarithmic post-culture. Finally, the biomass (1.248 g 路L ~ (- 1) and carbon sequestration rate (191.4 mg (L 路d) ~ (- 1) of Spirulina platensis were obtained by TEA optimization strategy, which was 25.6% and 41.2% higher than that of the control group, respectively.
【作者单位】：生物反应器工程国家重点实验室华东理工大学;
【分类号】：Q945;X173

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