一株高效丙烯腈降解细菌Rhodococus rhodochrous BX2的丙烯腈降解条件优化

发布时间：2019-04-19 02:55

【摘要】：【目的】以丙烯腈为目标污染物,利用实验室已筛选获得的一株高效腈降解菌Rhodococus rhodochrous BX2,研究其对丙烯腈的降解特性,优化降解条件以提高菌株对丙烯腈的降解能力。【方法】通过单因素试验和响应面分析相结合的方法优化Rhodococus rhodochrous BX2对丙烯腈的降解条件。考察外加碳、氮源对BX2的生长及丙烯腈降解的影响,并确定其在丙烯腈合成废水中对丙烯腈的处理效果。【结果】菌株BX2优化后的最佳降解条件为:底物浓度403.51 mg/L、p H 7.44、温度34.46°C,在此条件下丙烯腈的降解率为95.1%。外加碳源为葡萄糖,或外加氮源为氯化铵对菌株生长及丙烯腈降解有明显的促进作用。菌株Rhodococus rhodochrous BX2能够高效降解合成废水中的丙烯腈,在30 h时其丙烯腈降解率可达89.4%。【结论】降解条件优化以及外源物质的添加强化了菌株对丙烯腈合成废水的处理效果,为生物法处理丙烯腈废水新方法的开发提供技术支持。
[Abstract]:[objective] to study the degradation characteristics of acrylonitrile (Acrylonitrile) by a highly efficient nitrile decomposing bacterium, Rhodococus rhodochrous BX2, which has been screened in the laboratory, with acrylonitrile as the target pollutant. The degradation of acrylonitrile was improved by optimizing the degradation conditions. [methods] the degradation conditions of acrylonitrile by Rhodococus rhodochrous BX2 were optimized by single factor test and response surface analysis. The effects of carbon and nitrogen sources on the growth of BX2 and the degradation of acrylonitrile were investigated, and the treatment efficiency of acrylonitrile in the wastewater of acrylonitrile synthesis was determined. [results] the optimum degradation conditions of strain BX2 were as follows: substrate concentration 403.51 mg/L,. Ph 7.44, temperature 34.46 掳C, the degradation rate of acrylonitrile is 95.1% under this condition. The addition of glucose as carbon source or ammonium chloride as nitrogen source could obviously promote the growth and degradation of acrylonitrile. Strain Rhodococus rhodochrous BX2 could efficiently degrade acrylonitrile in synthetic wastewater, and the degradation rate of acrylonitrile could reach 89.4% in 30 h. [conclusion] the optimization of degradation conditions and the addition of exogenous substances strengthen the treatment effect of the strain on acrylonitrile synthesis wastewater. It provides technical support for the development of a new method for the treatment of acrylonitrile wastewater by biological process.
【作者单位】：东北农业大学资源与环境学院;
【基金】：国家自然科学基金项目(No.41271504)~~
【分类号】：X172

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