低浓度阿莫西林对铜绿微囊藻污染的调控效应与机制
发布时间：2019-05-23 04:56
【摘要】：由于人们持续使用和排放抗生素,天然水环境中频繁检出抗生素类污染物。蓝藻具有原核细胞结构,对抗生素的敏感程度很高。因此,除了氮、磷等常规的水环境因子,抗生素也可能成为调控蓝藻水华发生的新兴环境因子。本论文选择分布广泛的蓝藻水华模式种铜绿微囊藻为目标藻种,选择水环境中广泛检出的典型抗生素阿莫西林为目标抗生素,研究阿莫西林在现有污染水平下对铜绿微囊藻生长、产毒和多种生理功能的调控效应,通过半连续培养试验验证上述调控效应的长期性,并利用蛋白质组学手段深入探讨作用机制。阿莫西林的暴露浓度为100 ng/L和300 ng/L时,短期暴露条件下铜绿微囊藻叶绿素a含量与对照组相比显著增加(p0.05),且与光合作用相关的基因psbA、psaB和rbcL的表达量也都明显上调。指示光合作用活性的指标(Fv/Fm和rETRmax)在阿莫西林长期暴露条件下也呈现上升趋势。蛋白质组学分析结果进一步显示,psbA、psaB和rbcL基因编码的蛋白也发生上调表达。上述结果表明,无论是短期还是长期暴露,阿莫西林在环境浓度下均可以提高铜绿微囊藻的光合作用活性。光合作用受到刺激会增加能量合成,进而导致藻细胞的生长速率和微囊藻毒素的产量上升。这可能与低浓度阿莫西林对藻细胞的毒物兴奋效应相关。显著性富集的蛋白质功能模块显示,微囊藻毒素合成酶(mcyB)、泛应激蛋白(MAE_48380)和光合作用相关蛋白之间具有显著的相互作用(p0.05),这一结果进一步证明了 MCs合成、细胞应激和光合作用之间有密切关系。在低浓度阿莫西林暴露条件下,藻细胞的超氧化物歧化酶(SOD)、过氧化物酶(POD)和谷胱甘肽-S-转移酶(GST)的活性显著提高(p0.05),抗氧化剂谷胱甘肽(GSH)的含量也显著增加(p0.05),表明阿莫西林对藻细胞产生了氧化压力,并引发抗氧化系统应激反应。蛋白质组学分析进一步证明了上述结果。SOD的表达量上调,以及phaseⅠ蛋白(硫氧还蛋白过氧化物酶MAE_35830)和3个phase Ⅱ蛋白(谷胱甘肽-S-转移酶MAE_15850、谷胱甘肽还原酶MAE_46260和类糖基转移酶MAE_13100)的表达量上升,表明阿莫西林触发藻细胞的抗氧化应激及解毒反应。本文的研究结果表明,阿莫西林在其现有水环境污染浓度下,具有促进铜绿微囊藻暴发的潜在可能。
[Abstract]:Due to the continuous use and discharge of antibiotics, antibiotic pollutants are frequently detected in natural water environment. Blue algae have prokaryotic cell structure and are sensitive to antibiotics. Therefore, in addition to nitrogen, phosphorus and other conventional water environmental factors, antibiotics may also become a new environmental factor to regulate the occurrence of cyanobacteria Shui Hua. In this paper, the widely distributed blue algae Shui Hua model species Microcystis aeruginosa was selected as the target algae species, and amoxicillin, a typical antibiotic widely detected in water environment, was selected as the target antibiotic. To study the regulatory effects of amoxicillin on the growth, toxicity and various physiological functions of Microcystis aeruginosa at the existing pollution level, and to verify the long-term nature of the above regulatory effects through semi-continuous culture experiments. The mechanism of action was discussed by proteome. When the exposure concentration of amoxicillin was 100 ng/L and 300 ng/L, the chlorophyll a content of Microcystis aeruginosa under short-term exposure was significantly higher than that of the control group (p0.05), and the gene psbA, related to photosynthesis was significantly higher than that of the control group (p0.05). The expression of psaB and rbcL was also up-regulated. The indexes indicating photosynthetic activity (Fv/Fm and rETRmax) also showed an upward trend under long-term exposure to amoxicillin. Proteome analysis further showed that the proteins encoded by psbA,psaB and rbcL genes were also up-regulated. The above results showed that amoxicillin could increase the photosynthetic activity of Microcystis aeruginosa at environmental concentration, both short-term and long-term exposure. Photosynthesis stimulation increases energy synthesis, which in turn leads to an increase in the growth rate of algae cells and the production of microcystins. This may be related to the toxic excitatory effect of low concentration amoxicillin on algal cells. The significantly enriched protein functional module showed that there was a significant interaction between microcystin synthase (mcyB), pan-stress protein (MAE_48380) and photosynthesis-related proteins (p0.05). This result further proves that there is a close relationship between MCs synthesis, cell stress and photosynthesis. Under the condition of low concentration amoxicillin exposure, the activities of (POD) and (GST) of SOD (POD) and S-transferase in algae cells were significantly increased (p0.05). The content of glutathione (GSH) also increased significantly (p0.05), indicating that amoxicillin produced oxidative pressure on algal cells and triggered the stress response of antioxidant system. Proteome analysis further confirmed the above results. Sod expression was up-regulated, as well as phase 鈪，
本文编号：2483622
[Abstract]:Due to the continuous use and discharge of antibiotics, antibiotic pollutants are frequently detected in natural water environment. Blue algae have prokaryotic cell structure and are sensitive to antibiotics. Therefore, in addition to nitrogen, phosphorus and other conventional water environmental factors, antibiotics may also become a new environmental factor to regulate the occurrence of cyanobacteria Shui Hua. In this paper, the widely distributed blue algae Shui Hua model species Microcystis aeruginosa was selected as the target algae species, and amoxicillin, a typical antibiotic widely detected in water environment, was selected as the target antibiotic. To study the regulatory effects of amoxicillin on the growth, toxicity and various physiological functions of Microcystis aeruginosa at the existing pollution level, and to verify the long-term nature of the above regulatory effects through semi-continuous culture experiments. The mechanism of action was discussed by proteome. When the exposure concentration of amoxicillin was 100 ng/L and 300 ng/L, the chlorophyll a content of Microcystis aeruginosa under short-term exposure was significantly higher than that of the control group (p0.05), and the gene psbA, related to photosynthesis was significantly higher than that of the control group (p0.05). The expression of psaB and rbcL was also up-regulated. The indexes indicating photosynthetic activity (Fv/Fm and rETRmax) also showed an upward trend under long-term exposure to amoxicillin. Proteome analysis further showed that the proteins encoded by psbA,psaB and rbcL genes were also up-regulated. The above results showed that amoxicillin could increase the photosynthetic activity of Microcystis aeruginosa at environmental concentration, both short-term and long-term exposure. Photosynthesis stimulation increases energy synthesis, which in turn leads to an increase in the growth rate of algae cells and the production of microcystins. This may be related to the toxic excitatory effect of low concentration amoxicillin on algal cells. The significantly enriched protein functional module showed that there was a significant interaction between microcystin synthase (mcyB), pan-stress protein (MAE_48380) and photosynthesis-related proteins (p0.05). This result further proves that there is a close relationship between MCs synthesis, cell stress and photosynthesis. Under the condition of low concentration amoxicillin exposure, the activities of (POD) and (GST) of SOD (POD) and S-transferase in algae cells were significantly increased (p0.05). The content of glutathione (GSH) also increased significantly (p0.05), indicating that amoxicillin produced oxidative pressure on algal cells and triggered the stress response of antioxidant system. Proteome analysis further confirmed the above results. Sod expression was up-regulated, as well as phase 鈪，
本文编号：2483622
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