水稻镉敏感和耐性品种筛选及其在供铁下镉积累、分布的差异
发布时间：2021-02-10 08:26
镉污染已成为全球环境重要问题之一,而食用镉污染产品将给人体健康带来巨大威胁。大米是中国人重要主食来源之一,人类摄入镉的56%来源于大米。中国一些水稻主产区河流、湖泊及农田土壤受到镉污染,为最大限度地减少大米镉积累,本研究旨在通过评价在3个镉水下10个水稻品种生理生长、酶活性、镉吸收与分布、必需元素吸收积累等差异,获得镉敏感和耐性水稻品种,并进一步揭示镉敏感和耐性水稻品种对铁调节镉积累和分布的差异,为镉污染稻田安全利用和水稻安全生产提供依据。主要结果有:1)3个镉水平（0、50和100μm CdCl2）种子萌发试验结果表明,镉处理使10个水稻品种种子发芽率、幼苗生长参数和活力指数均有所下降,其中50μm镉处理的光1298和优838发芽率高于100μm镉处理;所有品种Cd积累量以100μm镉处理最大,而鲁9803和齐908Cd积累量较低。总体而言,鲁9803和齐908镉耐受性较强,光1298、万93和单63对镉较敏感。2)3个镉水平（0、50和100μm CdCl2）营养期试验结果表明,镉降低了10个水稻品种根、茎的长度和生物量,增加了抗氧化酶活...
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：130 页
【学位级别】：博士
【文章目录】：
Abstract
摘要
List of Abbreviations
1.REVIEW OF LITERATURE
1.1.CADMIUM POLLUTION IN SOIL
1.2.Cadmium intake by food items
1.3.Effect of Cadmium on Human Health:
1.4.Effect of Cd and Plant growth
1.5.Cadmium and oxidative stress
1.6.Research Progress
1.7.RICE
1.8.OBJECTIVES
1.8.1.Screening of rice cultivars with low Cd uptake under Cd stress
1.8.2.Differences in Cd distribution and accumulation between higher and lower Cd in ten rice cultivars
1.8.3.Uderstand how Fe application alleviates Cd toxicity,how cultivars accumulate low or high Cd
2.EFFECTS OF CD LEVELS ON SEED GERMINATION AND SEEDLING GROWTH OF TEN RICE CULTIVARS
2.1.INTRODUCTION
2.2.MATERIALS AND METHODS
2.2.1.Seed collection
2.2.2.Treatments and Experimental design
2.2.3.Cadmium analysis
2.2.4.Determination of parameters
2.2.5.Statistical analysis
2.3.RESULTS
2.3.1.Seedling Growth
2.3.2.Biomass
2.3.3.Germination Percentage
2.3.4.Seed vigor index
2.3.5.Relative water content
2.3.6.Cadmium Concentration and accumulation
2.4.DISCUSSION
2.5.CONCLUSION
3.EFFECT OF CADMIUM ON SEEDLING GROWTH,ANTIOXIDANT ENZYMES ACTIVITIES,FE,ZN AND CD CONCENTRATIONS OF TEN RICE CULTIVARS UNDER CD TOXICITY
3.1.INTRODUCTION
3.2.MATERIALS AND METHODS
3.2.1.Plant material
3.2.2.Plant growth and stress condition
3.2.3.Observations
3.2.4.Assays of enzymatic antioxidants
3.2.5.Elemental analysis
3.2.6.Statistical analysis
3.3.RESULTS
3.3.1.Seedling Growth
3.3.2.Plant biomass
3.3.3.Enzymatic activities
3.3.4.Iron distribution in rice plant
3.3.5.Zinc distribution in rice plant
3.3.6.Cd Concentration,Bio-Concentration Factor and Translocation Factor..
3.4.DISCUSSION
3.5.CONCLUSION
4.EFFECT OF FE ON SEEDLING GROWTH,ANTIOXIDANT ENZYMES ACTIVITY AND CD ACCUMULATION IN TWO RICE CULTIVARS UNDER THE PRESENCES OF CD TREATMENT
4.1.INTRODUCTION
4.2.MATERIALS AND METHODS
4.2.1.Experimental details
4.2.2.Observations
4.2.3.Determination of Cd and Fe concentration in plant
4.2.4.Assays of enzymatic antioxidants
4.3.RESULTS
4.3.1.Plant growth and Biomass
4.3.2.Cd concentration and accumulation
4.3.3.Fe Concentration and accumulation
4.3.4.SOD Activity
4.3.5.POD Activity
4.3.6.CAT Activity
4.4.DISCUSSION
4.5.CONCLUSION
5 VARIATION IN GROWTH,YIELD AND CD/FE ACCUMULATION,ULTRASTRUCTURAL CHANGES IN LEAF CELL OF TWO RICE CULTIVARS UNDER CD/FE TREATMENTS
5.1.INTRODUCTION
5.2.MATERIALS AND METHOD
5.2.1.Soil culture and experiment design
5.2.2 Pot set up and monitoring
5.2.3.Plant material and growth conditions
5.2.4.Observations:
5.2.5.Plant harvest
5.2.6.Determination of Cd and Fe concentration in plant
5.2.7.Calculation of BCF and TF
5.2.8.Statistical analysis
5.3.RESULTS
5.3.1.Plant biomass and yield
5.3.1.1.Fresh and dry biomass at various growth stages
5.3.1.2.Biological yield
5.3.1.3.1000-grain weight
-1)"> 5.3.1.4.Straw yield(g pot-1)
-1)"> 5.3.1.5.Grain Yield(g pot-1)
5.3.2.Fe Concentration and accumulation in rice plant
5.3.3.Cd concentration and accumulation in rice plant
5.3.3.1.Bio-concentration factors and transfer factor of Cd
5.3.4.Ultrastructure analysis of developing leaf of rice by Transmission Electron Microscopy
5.4 DISCUSSION
5.5.CONCLUSION
6.CONCLUSION AND FUTURE PERSPECTIVES
6.1.FUTURE DIRECTION
REFERENCES
ACKNOWLEDGEMENTS
本文编号：3027081
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：130 页
【学位级别】：博士
【文章目录】：
Abstract
摘要
List of Abbreviations
1.REVIEW OF LITERATURE
1.1.CADMIUM POLLUTION IN SOIL
1.2.Cadmium intake by food items
1.3.Effect of Cadmium on Human Health:
1.4.Effect of Cd and Plant growth
1.5.Cadmium and oxidative stress
1.6.Research Progress
1.7.RICE
1.8.OBJECTIVES
1.8.1.Screening of rice cultivars with low Cd uptake under Cd stress
1.8.2.Differences in Cd distribution and accumulation between higher and lower Cd in ten rice cultivars
1.8.3.Uderstand how Fe application alleviates Cd toxicity,how cultivars accumulate low or high Cd
2.EFFECTS OF CD LEVELS ON SEED GERMINATION AND SEEDLING GROWTH OF TEN RICE CULTIVARS
2.1.INTRODUCTION
2.2.MATERIALS AND METHODS
2.2.1.Seed collection
2.2.2.Treatments and Experimental design
2.2.3.Cadmium analysis
2.2.4.Determination of parameters
2.2.5.Statistical analysis
2.3.RESULTS
2.3.1.Seedling Growth
2.3.2.Biomass
2.3.3.Germination Percentage
2.3.4.Seed vigor index
2.3.5.Relative water content
2.3.6.Cadmium Concentration and accumulation
2.4.DISCUSSION
2.5.CONCLUSION
3.EFFECT OF CADMIUM ON SEEDLING GROWTH,ANTIOXIDANT ENZYMES ACTIVITIES,FE,ZN AND CD CONCENTRATIONS OF TEN RICE CULTIVARS UNDER CD TOXICITY
3.1.INTRODUCTION
3.2.MATERIALS AND METHODS
3.2.1.Plant material
3.2.2.Plant growth and stress condition
3.2.3.Observations
3.2.4.Assays of enzymatic antioxidants
3.2.5.Elemental analysis
3.2.6.Statistical analysis
3.3.RESULTS
3.3.1.Seedling Growth
3.3.2.Plant biomass
3.3.3.Enzymatic activities
3.3.4.Iron distribution in rice plant
3.3.5.Zinc distribution in rice plant
3.3.6.Cd Concentration,Bio-Concentration Factor and Translocation Factor..
3.4.DISCUSSION
3.5.CONCLUSION
4.EFFECT OF FE ON SEEDLING GROWTH,ANTIOXIDANT ENZYMES ACTIVITY AND CD ACCUMULATION IN TWO RICE CULTIVARS UNDER THE PRESENCES OF CD TREATMENT
4.1.INTRODUCTION
4.2.MATERIALS AND METHODS
4.2.1.Experimental details
4.2.2.Observations
4.2.3.Determination of Cd and Fe concentration in plant
4.2.4.Assays of enzymatic antioxidants
4.3.RESULTS
4.3.1.Plant growth and Biomass
4.3.2.Cd concentration and accumulation
4.3.3.Fe Concentration and accumulation
4.3.4.SOD Activity
4.3.5.POD Activity
4.3.6.CAT Activity
4.4.DISCUSSION
4.5.CONCLUSION
5 VARIATION IN GROWTH,YIELD AND CD/FE ACCUMULATION,ULTRASTRUCTURAL CHANGES IN LEAF CELL OF TWO RICE CULTIVARS UNDER CD/FE TREATMENTS
5.1.INTRODUCTION
5.2.MATERIALS AND METHOD
5.2.1.Soil culture and experiment design
5.2.2 Pot set up and monitoring
5.2.3.Plant material and growth conditions
5.2.4.Observations:
5.2.5.Plant harvest
5.2.6.Determination of Cd and Fe concentration in plant
5.2.7.Calculation of BCF and TF
5.2.8.Statistical analysis
5.3.RESULTS
5.3.1.Plant biomass and yield
5.3.1.1.Fresh and dry biomass at various growth stages
5.3.1.2.Biological yield
5.3.1.3.1000-grain weight
-1)"> 5.3.1.4.Straw yield(g pot-1)
-1)"> 5.3.1.5.Grain Yield(g pot-1)
5.3.2.Fe Concentration and accumulation in rice plant
5.3.3.Cd concentration and accumulation in rice plant
5.3.3.1.Bio-concentration factors and transfer factor of Cd
5.3.4.Ultrastructure analysis of developing leaf of rice by Transmission Electron Microscopy
5.4 DISCUSSION
5.5.CONCLUSION
6.CONCLUSION AND FUTURE PERSPECTIVES
6.1.FUTURE DIRECTION
REFERENCES
ACKNOWLEDGEMENTS
本文编号：3027081
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