Characterization of a Novel Salt Tolerant Bacterium Gracilib

发布时间：2024-05-16 03:31

　　

【文章页数】：172 页

【学位级别】：博士

【文章目录】：
Abstract
Acknowledgements
List of Tables
List of Figures
1.Introduction
2.Historical Background
    2.1.Salt Tolerant Bacteria
        2.1.1.Compatible solutes
            2.1.1.1.Proline
        2.1.2.Large extrachromosomal DNA
    2.2.Azo Dyes
    2.3.Reactive Azo Dyes
    2.4.Toxicity of Azo Dyes and Aromatic Amines
    2.5.Biotransformation of Azo Dyes
        2.5.1.Biotransformation of azo dyes under aerobic conditions
        2.5.2.Biotransformation of azo dyes under anaerobic conditions
        2.5.3.Biotransformation of reactive azo dyes
        2.5.4.Treatment of azo dye containing wastewaters
3.Phases of Study
    3.1.Phase Ⅰ
    3.2.Phase Ⅱ
    3.3.Phase Ⅲ
    3.4.Phase Ⅳ
    3.5.Phase Ⅴ
4.Isolation,Identification and Characterization of the Bacterial Strain Gracilibacillus sp.GTY
    4.1.Introduction
    4.2.Materials and Methods
        4.2.1.Media and culture conditions
        4.2.2.Extraction of genomic DNA,16S rDNA PCR,sequencing and phylogenic analysis
    4.3.Results and Discussion
        4.3.1.Isolation,identification and characterization
            4.3.1.1.Growth characteristics of GTY
                4.3.1.1.1.Effect of NaCl concentrations on growth
                4.3.1.1.2.Effect of pH variations on growth
                4.3.1.1.3.Screening of compatible solutes
    4.4.Conclusions
5.Study of Large Extrachromosomal DNA
    5.1.Introduction
    5.2.Materials and Methods
        5.2.1.Media and culture condition
        5.2.2.Isolation technique of the extrachromosomal DNA
        5.2.3.Elimination technique of the extrachromosomal DNA
        5.2.4.Estimation
    5.3.Results and Discussion
        5.3.1.Isolation of the extrachromosomal DNA
        5.3.2.Elimination of the extrachromosomal DNA
        5.3.3.Effect of this extrachromosomal DNA on the host bacterial salt tolerance,dye decolorization and antibiotic sensitivity
    5.4.Conclusions
6.Selection and Characterization of the Dyes
    6.1.Introduction
    6.2.Characterization of used dyes
    6.3.Selection of Acid Red B for more detailed investigations
        6.3.1.Effect of salt on UV-visible spectrum of Acid Red B
7.Decolorization of Various Dyes(lab-scale dye solutions)by the Strain GTY
    7.1.Introduction
    7.2.Materials and Methods
        7.2.1.Media and culture conditions
        7.2.2.Decolorization by growing cells
            7.2.2.1.Decolorization in anaerobic liquid media
            7.2.2.2.Decolorization on agar plate
        7.2.3.Decolorization by resting cells
        7.2.4.Decolorization by extracted azo reductase
        7.2.5.Assay
    7.3.Results and Discussion
        7.3.1.Decolorization of the used dyes by growing cells
        7.3.2.Decolorization of selected azo dye Acid Red B by growing cells
            7.3.2.1.Decolorization of Acid Red B on LB agar plate versus in LB liquid media
            7.3.2.2.Decolorization under different NaCl concentrations
            7.3.2.3.Effect of carbon source on decolorization
            7.3.2.4.Effect of pH on decolorization
            7.3.2.5.Effect of light on decolorization
        7.3.3.Decolorization of selected azo dye Acid Red B by resting cells.
        7.3.4.Decolorization of selected azo dye Acid Red B by extracted azo reductase
    7.4.Conclusions
8.Treatment of Real Textile Wastewater by the Strain GTY
    8.1.Introduction
    8.2.Materials and Methods
        8.2.1.Textile wastewater
        8.2.2.Operation technique
        8.2.3.Analytical methods
    8.3.Results and Discussion
        8.3.1.Decolorization of textile wastewater samples
        8.3.2.Decolorization and COD removal in presence and absences of carbon sources
        8.3.3.Effect of pH on decolorization and COD removal
        8.3.4.Effect of temperature on decolorization and COD removal
    8.4.Conclusions
9.Conclusions and Recommendations for Further Studies
References
Scientific Articles
Appendix Ⅰ:Abbreviations
Appendix Ⅱ:16S rDNA Sequence Survey of the Strain GTY
Appendix Ⅲ:GenBank of 16S rDNA Sequence of the Strain GTY



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