氧化石墨烯复合纳米材料修饰的生物小分子传感器的制备及应用

发布时间：2017-12-31 14:38

本文关键词：氧化石墨烯复合纳米材料修饰的生物小分子传感器的制备及应用　出处：《天津工业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本文设计了一种基于氧化石墨烯和壳聚糖复合纳米材料修饰的电化学DNA传感器,对大肠杆菌0157:H7的DNA序列进行了分析检测。通过改良的Hummers法制备氧化石墨烯,并通过红外光谱、透射电子显微镜、扫描电子显微镜、X射线衍射仪进行表征。利用合成的氧化石墨烯与壳聚糖通过静电吸附及化学键结合的方式组装合成氧化石墨烯-壳聚糖复合材料,并用其直接修饰玻碳电极制备DNA传感器。通过循环伏安法探究氧化石墨烯与壳聚糖的最佳比例为0.4wt%。在最优杂交条件下,利用交流阻抗法对传感器的组装过程及目标DNA的检测进行表征。并以亚甲基蓝作为杂交指示剂,通过差分脉冲伏安法探究了传感器的选择性。该传感器的检测范围为该传感器的检测范围为1.0×10-11-1.0×10-7 M,检出限为 5.372×10-13M(3σ,n=5)。本文设计了一种基于氧化石墨烯和金纳米粒子修饰玻碳电极的电化学DNA传感器,对膀胱癌DNA进行电化学检测。利用氧化石墨烯和金纳米粒子复合材料修饰玻碳电极,制备得到电化学DNA传感器。亚甲基蓝为杂交指示剂,通过扫描电子显微镜、循环伏安法、差分脉冲伏安法对传感器的组装过程及目标DNA的检测进行表征。所得检测范围为1.0×10-11-1.0×10-7M,检出限为5.372×10-13M(3σ,n=5)。本文初步设计了一种基于氧化石墨烯与二氧化钛复合纳米材料修饰的电化学DNA传感器。通过透射电子显微镜、扫描电子显微镜、循环伏安法、差分脉冲伏安法对传感器的组装过程及目标DNA的检测进行了表征。
[Abstract]:This paper describes the design of a DNA electrochemical sensor of graphene oxide and chitosan nanocomposite modified based on the DNA sequence of Escherichia coli 0157:H7 were detected. The preparation of graphene oxide by modified Hummers method, and through infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, were characterized with X ray diffraction. Through electrostatic adsorption and chemical bond using graphene oxide and chitosan synthesis way assembly synthesis of graphene oxide / chitosan composite materials, and used directly modified glassy carbon electrode for DNA sensor. The optimum ratio by cyclic voltammetry on graphene oxide and chitosan was 0.4wt%. under the optimal hybridization conditions, characterization were detected by EIS and DNA assembly process on the sensor. And using methylene blue as hybridization indicator, by differential pulse voltammetry research The sensor is selective. The detecting range of the sensor for the detection range of the sensor is 1 * 10-11-1.0 * 10-7 M, a detection limit of 5.372 * 10-13M (3, n=5). This paper introduces a design based on graphene oxide and gold nanoparticles modified glassy carbon electrode electrochemical DNA sensor for electrochemical detection of bladder cancer DNA. The use of graphene oxide and gold nanoparticles composite modified glassy carbon electrode was prepared by electrochemical DNA sensor. Methylene blue as hybridization indicator, by scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry detection of sensor assembly process and the target of DNA were characterized. The detection range is 1 * 10-11-1.0 * 10-7M with a detection limit of 5.372 * 10-13M (3, n=5). This paper designs a kind of electrochemical DNA biosensor modified graphene oxide and titanium dioxide nano composite materials based on the transmission. Electron microscopy, scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry have been used to characterize the assembly process of the sensor and the detection of the target DNA.

【学位授予单位】：天津工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;TP212

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