土壤放线菌XN-04代谢产物除草活性成份分离纯化与结构解析

发布时间：2018-01-07 05:08

本文关键词：土壤放线菌XN-04代谢产物除草活性成份分离纯化与结构解析　出处：《华东理工大学》2017年硕士论文　论文类型：学位论文

【摘要】：通过活性筛选,得到土壤放线菌XN-04,其发酵的次级代谢产物经过生物测定具有良好的除草活性。本文研究了其具有抑制杂草发芽生长活性的次级代谢产物,并通过相关手段对其进行了菌种鉴定。对该菌株XN-04的发酵次级代谢产物依次进行溶剂萃取、硅胶柱层析、薄层层析、高效液相制备等分离提纯手段,分离得到了两个具有除草活性的化合物XN-04-A4和XN-04-B2。两个化合物经过质谱(MS)、核磁共振(NMR)等常规的波谱测试,最终解析出两个化合物的分子式分别是C15H10O5和C15H23NO4,相关化学分子量分别为270和281。综合各类谱图分析,确定化合物XN-04-A4与文献报道的染料木素(Genistein)结构相同;化合物XN-04-B2与文献报道的放线菌酮(Naramycin A)结构相同。通过研究,建立了用高效液相色谱法测定发酵液中化合物XN-04-A4和XN-04-B2的发酵效价,并进行了化合物XN-04-A4和XN-04-B2除草谱的试验。通过观察菌株XN-04的培养特征、形态特征、生理生化特征,综合16SrDNA基因序列分析,确定该菌株归属于链霉菌属,与Streptomycesgalilaeus同源性相似度为99%。
[Abstract]:Soil actinomycetes XN-04 was obtained by activity screening. The secondary metabolites of its fermentation have good herbicidal activity by bioassay. In this paper, the secondary metabolites which have the ability to inhibit the growth of weeds were studied. The fermentation secondary metabolites of XN-04 were separated and purified by solvent extraction, silica gel column chromatography, thin layer chromatography, high performance liquid phase preparation and so on. Two herbicide compounds, XN-04-A4 and XN-04-B2, were isolated. The two compounds were characterized by conventional spectroscopic measurements such as MS MS, NMR and so on. Finally, the molecular formulas of the two compounds are C15H10O5 and C15H23NO4, the molecular weights of which are 270 and 281, respectively. It was confirmed that the structure of the compound XN-04-A4 was the same as that of genistein reported in the literature. The structure of compound XN-04-B2 is the same as that of Naramycin A reported in the literature. The fermentation titers of compounds XN-04-A4 and XN-04-B2 in fermentation broth were determined by HPLC. The experiment of compound XN-04-A4 and XN-04-B2 weeding spectrum was carried out. The culture characteristics, morphological characteristics, physiological and biochemical characteristics of XN-04 were observed. According to the analysis of 16s rDNA gene sequence, the strain belongs to Streptomyces, and the similarity with Streptomycesgalilaeus is 99%.
【学位授予单位】：华东理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ450.1;TQ920.1

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