费约果香气成分研究及生物活性初探

发布时间：2018-01-18 06:30

  本文关键词：费约果香气成分研究及生物活性初探　出处：《西南科技大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 费约果 香气 提取工艺 成分分析 抗氧化性 抑菌活性

【摘要】：为了对费约果香气成分有全面认识,文章采用顶空固相微萃取结合气相色谱-质谱(HS-SPME-GC-MS)对费约果不同品种不同器官香气成分进行了分析,并对费约果叶片精油生物活性进行了初探。主要研究内容和结果如下:1、采用三种不同方法(索氏抽提法、超声波辅助溶剂萃取法、顶空固相微萃取法)对费约果叶片香气进行萃取,经气相色谱-质谱(GC-MS)分析后发现,三种方法分别能检测到36、26和63种物质。就种类而言,顶空固相微萃取法能得到最多的香气成分。就性质而言,顶空固相微萃取法直接顶空吸附进样,萃取的香气组分最接近植物体散发的香气。2、采用顶空固相微萃取法,以75μm CAR/PDMS萃取头为吸附介质,设计L9(34)正交试验对萃取条件进行优化,以出峰个数和总峰面积为考察指标,进行极差分析。得出最佳实验条件为:加样量0.6 g,萃取温度50℃,萃取时间50 min,解析时间6 min。在此条件下,可分离得到费约果叶片中88种物质,鉴定出其中65种,占香气总量的96.583%,优于正交试验中任何一组,说明选出的萃取条件切实可行,能最大程度反映香气构成。3、以“2”中优化所得萃取条件,采用顶空固相微萃取法(HS-SPME)结合气相色谱-质谱(GC-MS),对费约果不同品种不同器官香气进行萃取分析,共可在叶片中鉴定出114种香气成分,全果中鉴定出87种香气成分,花朵中鉴定出106种香气成分,分析发现,不同品种不同器官间香气差异较大。4、采用溶剂萃取法提取费约果叶片精油,分别对其进行超氧阴离子自由基、羟基自由基、DPPH自由基清除实验和脂质过氧化抑制实验,以抗坏血酸为标准品,探究精油抗氧化活性;以食品杀菌中常用的目标菌大肠杆菌和金黄色葡萄球菌为供试菌,探究精油抑菌活性。结果表明,费约果叶片精油对超氧阴离子自由基、羟基自由基、DPPH自由基表现出了较强的清除能力,对脂质过氧化也表现出了较强的抑制能力,且对DPPH自由基清除力和脂质过氧化抑制力远远强于抗坏血酸标准品,对超氧阴离子自由基和羟基自由基的清除力略低于抗坏血酸标准品。同时,费约果叶片精油对大肠杆菌和金黄色葡萄球菌皆表现出了一定的抑制作用,且对大肠杆菌的抑制作用强于金黄色葡萄球菌。
[Abstract]:In order to have a comprehensive understanding of the aroma components of Feryogo. Headspace solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to analyze the aroma components of different varieties and organs of Feiyogu. The biological activity of essential oil from Feryogor leaves was studied. The main contents and results were as follows: 1. Three different methods (Soxhlet extraction and ultrasonic assisted solvent extraction) were used. Headspace solid-phase microextraction (SPME) was used to extract the aroma of Feyoguo leaves. After GC-MS analysis, it was found that 36% could be detected by the three methods respectively. 26 and 63 kinds of substances. Headspace solid phase microextraction method can obtain the most aroma components. In terms of properties, headspace solid phase microextraction method can directly adsorb samples in headspace. The aroma component of extraction is closest to that of plant. 2. Headspace solid phase microextraction (SPME) method with 75 渭 m CAR/PDMS head as adsorption medium was used. The extraction conditions were optimized by the orthogonal design of L9 ~ (34). The optimum experimental conditions were obtained as follows: the amount of sample was 0.6 g, and the total peak area and the number of peaks were taken as the index of investigation. The extraction temperature was 50 鈩，

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