季铵化卤胺聚合物改性纳米金刚石的制备与性能研究

发布时间：2018-02-24 17:23

本文关键词： 季铵盐卤胺纳米金刚石分散性抗菌再生性　出处：《南京理工大学》2017年硕士论文　论文类型：学位论文

【摘要】：近年来,微生物威胁人类安全和健康成为社会关注的焦点。抗菌材料作为有效抑制细菌感染的手段,其研发尤其是新型抗菌材料的研发不断引起重视。抗菌材料广泛应用于水净化系统、医疗器械、食品包装、食物保鲜、卫生设施等领域内。纳米金刚石具有优异的力学、光学、热学性质,有广阔的应用前景,最近其在抗菌领域的研究得到关注。卤胺化合物因具有良好的广谱杀菌性、稳定性和再生性,对人体和环境安全等优点而受到关注,卤胺通过释放氧化态氯离子,破坏细胞基质,促使细菌死亡。季铵盐类抗菌剂特别是高分子季铵盐表面由于其带有的丰富正电荷可以吸附到细菌表面并杀死细菌,研究表明季铵盐基团表面电荷可以改善纳米粒子分散性能。本文以纳米金刚石为载体通过两种方法制备了两种高分子季铵盐型卤胺改性的纳米金刚石复合粒子,并对其化学组成、分散性能和抗菌能力等进行了深入研究。具体研究内容如下:1、聚阳离子型卤胺改性的纳米金刚石复合抗菌材料(PCN-NDs)。以纳米金刚石为载体,用季铵化卤胺高分子对其成功改性,制备了聚阳离子型卤胺改性的纳米金刚石颗粒。利用红外光谱(FT-IR)、核磁共振氢谱(1HNMR)、透射电镜(TEM)、热重分析(TG)以及X-射线光电子能谱(XPS)等对纳米颗粒的化学组成、分散性能等进行表征分析。通过抗菌动力学实验和最小抑菌浓度测试等手段来评价比较所制备抗菌材料的抗菌性能。实验结果表明:聚阳离子改性对纳米粒子具有明显的解团聚效果,大幅提升了分散性能,可以将纳米金刚石团聚体分离成40-50nm。PCN-NDs抗菌纳米颗粒在和细菌接触15 min时间内即可杀死100%的E.coli,5min内杀死100%的S.aureus。此外还发现PCN-NDs具备良好的再生性和储存稳定性。2、卤胺改性壳聚糖/纳米金刚石复合抗菌材料(Cl-DMH-CTS-NDs)。以纳米金刚石为载体,在纳米金刚石表面组装卤胺改性壳聚糖,成功制备卤胺改性壳聚糖/纳米金刚石颗粒。利用红外光谱(FT-IR)、核磁共振氢谱(1HNMR)透射电镜(TEM)、热重分析(TG)以及Zeta电位等对纳米复合粒子的化学组成、分散性能等进行表征分析。通过抗菌动力学测试、最小抑菌浓度测试等手段来评价比较制备抗菌材料的抗菌性能。实验结果表明,改性方案有效的改善了纳米金刚石的分散稳定性;Cl-DMH-CTS-NDs抗菌纳米颗粒在和细菌接触30 min的时间内,即可杀死100%的E.coli和的S.aaureus。Cl-DMH-CTS-NDs具备良好的再生性,释放活性氯后能够通过简单的氯化再生。
[Abstract]:In recent years, microbial threats to human safety and health have become the focus of attention of the community. Antimicrobial materials as an effective means of inhibiting bacterial infection, Antibacterial materials are widely used in water purification systems, medical devices, food packaging, food preservation, sanitary facilities and other fields. Nanocrystalline diamond has excellent mechanics, optics, etc. Its thermal properties have broad application prospects, and its recent research in the field of antimicrobial has attracted attention. Halogenamine compounds have attracted much attention because of their advantages of good broad-spectrum bactericidal properties, stability, reproducibility, and safety to human body and environment. Halamine kills bacteria by releasing oxidized chlorine ions, destroying the cell matrix and causing bacteria to die. Quaternary ammonium salts, especially macromolecular quaternary ammonium salts, can be adsorbed on the surface of bacteria and killed because of their rich positive charges. The results show that the surface charge of quaternary ammonium salt group can improve the dispersion of nanoparticles. In this paper, two kinds of high molecular quaternary ammonium salt modified nanocrystalline diamond composite particles were prepared by two methods using nanocrystalline diamond as the carrier. The chemical composition, dispersion and antimicrobial ability of nanocrystalline diamond were studied in detail as follows: 1, polycationic halogenamine modified nano-diamond composite antibacterial material PCN-NDs1.The nanocrystalline diamond was used as the carrier. The quaternary ammonium halogen amine polymer was successfully modified. Nano-diamond particles modified by polycationic halogenamine were prepared. The chemical composition of nanocrystalline diamond particles was studied by FTIR, 1H NMR, TEM, TG) and X- ray photoelectron spectroscopy (XPS). The antibacterial properties of the prepared antimicrobial materials were evaluated and compared by means of antibacterial kinetic test and minimal inhibitory concentration test. The experimental results showed that polycationic modification had good properties on nanoparticles. It has obvious effect of unreuniting. Greatly improved dispersion performance, Nanocrystalline diamond aggregates can be separated into 40-50nm.PCN-NDs antibacterial nanoparticles, which can kill 100% of E. coli and 100% S.aureus within 5 minutes after contact with bacteria for 15 min. In addition, it is also found that PCN-NDs has good regeneration and storage stability of 0.2Halogen modified. Chitosan / nanocrystalline diamond composite antibacterial material Cl-DMH-CTS-NDs0.Using nanocrystalline diamond as carrier, Halogenamine modified chitosan was assembled on the surface of nanocrystalline diamond. Halogenamine modified chitosan / nanocrystalline diamond particles were successfully prepared. The chemical composition of nano-composite particles was studied by FTIR, 1H NMR, TEM, TG) and Zeta potential. The antibacterial properties of the prepared antimicrobial materials were evaluated and compared by means of antibacterial kinetic test, minimal inhibitory concentration test and so on. The experimental results showed that, The modified method can effectively improve the dispersion stability of nanocrystalline diamond. Within 30 min of contact with bacteria, Cl-DMH-CTS-NDs can kill 100% E. coli and S.aaureus.Cl-DMH-CTS-NDs. After releasing active chlorine, it can be regenerated by simple chlorination.
【学位授予单位】：南京理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB34

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