氧化石墨烯涂层制备及抗菌性能研究

发布时间：2018-03-09 20:50

本文选题：硅橡胶　切入点：氧化石墨烯　出处：《太原理工大学》2017年硕士论文　论文类型：学位论文

【摘要】：硅橡胶由于其自身的理化稳定性、生理惰性和耐老化等优良的特性而广泛应用于植入材料,但其表面易于发生细菌黏附,进而形成生物膜,引发感染和多种并发症,使病人承受极大的痛苦。在硅橡胶表面制备抗菌涂层是减少这类细菌感染的简单而有效的手段。但常用的抗菌剂如银离子等抗菌涂层在有效地阻止细菌黏附、增殖的同时,存在细胞毒性等潜在危险,其应用受到了限制。为此,为获得安全、有效的抗菌涂层,本研究在硅橡胶基体上经化学改性后将氧化石墨烯接枝在其表面,形成氧化石墨烯抗菌涂层。首先通过紫外辐照对硅橡胶表面进行活化,使其表面产生活性基团,随后在其表面接枝硅烷偶联剂(?-巯丙基)三甲氧基硅烷,从而使氧化石墨烯与硅橡胶表面牢固键和,形成抗菌涂层。为了增大氧化石墨烯比表面积,利用溶致液晶软模板制备出有序纳米结构氧化石墨烯,并将其接枝在硅橡胶基体表面,形成有序结构氧化石墨烯抗菌涂层。利用拉曼光谱(Raman Spectra)、同步辐射小角X-射线散射(SAXS)表征氧化石墨烯微观结构;采用扫描电子显微镜(SEM)观察样品表面的微观形貌;通过接触角测量仪来观察样品表面亲疏水性变化;采用偏光显微镜(POM)和低角度X-射线衍射(Low-angle XRD)表征溶致液晶模板结构;采用低角度XRD和透射电子显微镜(TEM)测定结构化氧化石墨烯的有序结构;利用平板菌落计数法和荧光染色法评价氧化石墨烯涂层及有序结构氧化石墨烯涂层的抗菌性能;通过细胞活死染色法检测细胞死活,判定氧化石墨烯抗菌涂层对内皮细胞的细胞毒性。以上实验得到的主要结论有:(1)通过紫外辐照及硅烷偶联剂的桥接作用,将氧化石墨烯接枝在硅橡胶基体表面,形成氧化石墨烯涂层。(2)硅橡胶基体表面的氧化石墨烯涂层对大肠杆菌和金黄色葡萄球菌都表现出了抗菌性,且氧化石墨烯涂层对大肠杆菌和金黄色葡萄球菌的抗菌性更多地来源于其氧化应激作用,而不仅仅是机械物理损伤。(3)硅橡胶基体表面的氧化石墨烯涂层对内皮细胞没有表现出明显的细胞毒性。(4)利用溶致液晶软模板可成功制备出层状和六方有序结构氧化石墨烯。(5)与硅橡胶基体表面的氧化石墨烯涂层相比,有序结构氧化石墨烯涂层的抗菌性有所增强。
[Abstract]:Silicone rubber is widely used in implant materials because of its physical and chemical stability, physiological inertia and aging resistance. However, bacterial adhesion is easy to occur on the surface of silicone rubber, resulting in biofilm, infection and many complications. The preparation of antibacterial coatings on silicone rubber surfaces is a simple and effective means to reduce such bacterial infections. But common antimicrobial agents such as silver ions can effectively prevent bacteria from adhering and proliferating. In order to obtain a safe and effective antibacterial coating, graphene oxide was grafted onto the surface of silicone rubber matrix after chemical modification. The surface of silicone rubber was first activated by ultraviolet irradiation to produce active groups, and then the silane coupling agent was grafted on the surface. In order to increase the specific surface area of graphene oxide, graphene oxide was prepared by lyotropic liquid crystal soft template. It was grafted on the surface of silicone rubber to form an ordered graphene oxide antibacterial coating. The microstructure of graphene oxide was characterized by Raman Spectra-Raman spectroscopy and SAXS. Scanning electron microscopy (SEM) was used to observe the microstructure of the sample surface, the hydrophobicity of the sample surface was observed by contact angle measuring instrument, the structure of lyotropic liquid crystal template was characterized by polarizing microscope (POM) and low-angle X-ray diffraction (Low-angle XRD). The ordered structure of structured graphene oxide was determined by low angle XRD and transmission electron microscope (TEM), and the antibacterial properties of graphene oxide coating and ordered graphene oxide coating were evaluated by plate colony counting and fluorescence staining. The cytotoxicity of graphene oxide coating on endothelial cells was determined by the method of cell death staining. The main conclusions of the above experiments are: 1) bridging by ultraviolet irradiation and silane coupling agent. Graphene oxide was grafted on the surface of silicone rubber to form graphene oxide coating. 2) the graphene oxide coating on the surface of silicone rubber showed antibacterial activity against Escherichia coli and Staphylococcus aureus. Moreover, the antibacterial activity of graphene oxide coating against Escherichia coli and Staphylococcus aureus was mainly due to its oxidative stress. Not only mechanical and physical damage. (3) graphene oxide coating on silicone rubber substrates showed no obvious cytotoxicity to endothelial cells. 4) layered and hexagonal ordered oxygen could be successfully prepared by using lyotropic liquid crystal soft template. Compared with the graphene oxide coating on the surface of silicone rubber, The antibacterial properties of the ordered graphene oxide coating were improved.
【学位授予单位】：太原理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB306;R318.08

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