静电纺载药胶原蛋白纳米纤维膜的制备及性能研究

发布时间：2018-06-16 21:05

本文选题：载药纤维 + 静电纺丝技术　；参考：《天津工业大学》2017年硕士论文

【摘要】：载药纳米纤维是一种被广泛利用于靶细胞运输和释放的药物载体,在给药过程中使药物缓慢释放,因此备受关注。本文通过静电纺丝技术制备了胶原蛋白纳米纤维膜,分别用EDC/NHS和柠檬酸交联体系对其进行交联改性,并对交联前后的纳米纤维膜进行了结构和性能研究。通过静电纺丝技术,在电压20 kv、接收距离15 cm、挤出速率0.8 m1/h、纺丝溶液浓度9%的纺丝条件下,制备出的纳米纤维光滑,并且直径分布均匀。EDC/NHS和柠檬酸交联体系交联后的纳米纤维,通过FSEM观察到了明显的交联网络存在。通过FT-IR对比发现,交联处理并未改变胶原蛋白原有的三螺旋结构,因此不会改变胶原蛋白的优异性能。通过对比交联前后的水接触角,发现交联后的纳米纤维的亲水性并未发生改变。用考马斯亮蓝法结合紫外分光光度计对交联前后纳米纤维的降解行为和载药前后的药物释放行为进行了表征和对比。交联前的纳米纤维降解时间为10天,交联后的纳米纤维降解时间提高到了 21天,耐水性也有显著的提高。在最佳纺丝条件下,制备了载药胶原蛋白纳米纤维膜,采用FSEM和TEM对纤维形貌及内部结构进行了表征。结果表明,在纳米纤维表面和内部都观察不到5-FU药物颗粒的存在,说明5-FU与胶原蛋白的相容性极好,分子级均匀分布于胶原蛋白纤维中。通过研究交联前后的载药纳米纤维药物释放,发现交联后的载药纤维的药物突释现象得到了明显的降低,药物利用率提高了 10%-30%。采用这种交联后的纳米纤维进行给药,能够减轻患者的痛苦和不便,是一种很有前景的给药方式。
[Abstract]:Drug-loaded nanofibers are widely used as drug carriers for the transport and release of target cells. In this paper, collagen nanofilament membranes were prepared by electrospinning technique and were crosslinked by EDC / NHS and citric acid crosslinking system respectively. The structure and properties of the nanofibers before and after crosslinking were studied. Under the conditions of 20 kv voltage, 15 cm receiving distance, 0.8 ml / h extrusion rate and 9% concentration of spinning solution, the nanofibers prepared by electrospinning technology are smooth, and the diameter distribution is even. EDC / NHS is crosslinked with citric acid crosslinking system. The existence of cross-linking network was observed by FSEM. By FT-IR comparison, it was found that the crosslinking treatment did not change the original triple helix structure of collagen, so it would not change the excellent performance of collagen. By comparing the water contact angle before and after crosslinking, it is found that the hydrophilicity of the crosslinked nanofibers has not changed. Coomassie brilliant blue method and UV spectrophotometer were used to characterize and compare the degradation behavior and drug release behavior of nanofibers before and after crosslinking. The degradation time of the nanofibers before crosslinking was 10 days, and the degradation time of the cross-linked nanofibers increased to 21 days, and the water resistance was also significantly improved. Under the optimum spinning conditions, the drug-loaded collagen nanofibers were prepared, and the morphology and internal structure of the fibers were characterized by FSEM and TEM. The results showed that the presence of 5-FU drug particles was not observed on the surface and inside of nanofibers, indicating that 5-FU had excellent compatibility with collagen, and was evenly distributed in collagen fibers at molecular level. By studying the drug release of drug-loaded nanofibers before and after crosslinking, it was found that the drug sudden release of the cross-linked fibers was obviously reduced, and the drug utilization rate was increased by 10-30%. This cross-linked nanofiber can alleviate the pain and inconvenience of patients and is a promising drug delivery method.
【学位授予单位】：天津工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ340.64;TQ460.4

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