葡萄果实单萜化合物含量的QTL定位及其合成调控的候选基因筛选

发布时间：2017-12-28 01:05

本文关键词：葡萄果实单萜化合物含量的QTL定位及其合成调控的候选基因筛选　出处：《中国科学院武汉植物园》2017年博士论文　论文类型：学位论文

更多相关文章：葡萄 遗传图谱 QTL定位 单萜 候选基因

【摘要】：玫瑰香香味是葡萄和葡萄酒重要的品质性状,遗传图谱的构建是数量性状定位和研究的基础,随着分子标记技术的飞速发展,葡萄已经公布了 40多张遗传连锁图谱,这些图谱由不同的亲本构建,已用于各种数量性状的扫描,本文以'北丰'(中性品种)作为母本,'3-34'(玫瑰香品种)作为父本杂交的150个后代为材料,采用GBS简化基因组测序技术,分别构建了 '北丰'和'3-34'的高密度遗传图谱,对玫瑰香味性状进行QTL定位,结合RNA-seq技术,初步筛选出一些与玫瑰香香味物质合成相关的候选基因,主要研究成果如下:1.分别构建了父本'3-34'和母本'北丰'的遗传图谱,其中,父本'3-34'分子遗传图谱形成19个连锁群,包含了 1921个SNP标记,总图距为1696.8 cM,图谱覆盖率84.3%,平均图距为1cM;母本'北丰'形成19个连锁群,包含了2769个SNP标记,总图距为1810.2 cM,图谱覆盖率78.3%,平均图距为0.65 cM。2.完成对'北丰' × '3-34'杂交后代三年香气的分析(2011-2013),分析杂交后代的分离特点,并且估计广义遗传值。其中'北丰'测定出28种香气物质,其中C6化合物是主要的呈香物质,'3-34'测定出36种香气物质,其中单萜是主要的含香物质,其中白柠檬醚,玫瑰醚,橙花醇三种萜类物质在后代的分离比符合孟德尔遗传规律,分别是1:1,1:3,1:1。3.对17种单萜类物质进行QTL扫描,定位了 73个QTL位点,发现大部分QTL位点可控制多种单萜物质,综合QTL和RNA-Seq分析的结果,确定了 32个候选基因可能参与单萜代谢。4.通过对转录因子bZip61过量表达,可以使石竹烯,香叶醇和橙花醇含量升高,说明转录因子bZip61可能与调控单萜合成。
[Abstract]:Rose fragrance is an important and Wine grape quality traits, genetic map construction is the basis of the quantitative traits and positioning research, with the rapid development of molecular marker technology, grape has released more than 40 linkage maps, the map constructed by different parents, has been used for quantitative traits of various scanning, the 'North Feng' (neutral varieties) as the female parent,'3-34'(rose varieties) as 150 offspring male parent of hybrid as materials, using the simplified GBS genome sequencing technology, constructed a high density genetic map' Bei Feng 'and'3-34' spectrum, QTL positioning of the rose aroma characters, combined with RNA-seq technology, and some preliminary screening rose aroma substances associated with the synthesis of candidate genes, the main research results are as follows: 1. constructed genetic map, the male and female parent ''3-34' 'which Bei Feng, male form 19'3-34' molecular genetic map The linkage group contains 1921 SNP markers, the total distance is 1696.8 cM, the map coverage rate is 84.3%, the average map spacing is 1cM, the female parent 'Bei Feng' has 19 linkage groups, including 2769 SNP markers, the total distance is 1810.2 cM, the map coverage rate is 78.3%, and the average distance is 0.65 cM. 2. the analysis of the three year aroma of the hybrid offspring of 'North Feng' *'3-34'(2011-2013) was completed. The characteristics of the hybrid progeny were analyzed and the generalized genetic value was estimated. The 'North' Feng determined 28 aroma compounds, which C6 is the main aroma producing substances,'3-34'determination of 36 kinds of aroma substances, which is fragrant substances containing mainly monoterpenes, including lime ether, ether rose, Neroli three kinds of terpenoids in the offspring of Mendel genetic segregation ratio the law, which are 1:1,1:3,1:1. 3. QTL scanning of 17 monoterpenes was carried out. 73 QTL loci were located. It was found that most QTL sites could control multiple monoterpenes. Combined with QTL and RNA-Seq analysis, 32 candidate genes were identified to be involved in monoterpene metabolism. 4. by overexpression of the transcription factor bZip61, the content of carianenol, geraniol and Neroli could be increased, indicating that the transcription factor bZip61 may be synthesized with the regulation of monoterpenes.
【学位授予单位】：中国科学院武汉植物园
【学位级别】：博士
【学位授予年份】：2017
【分类号】：S663.1;Q943.2

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