拟南芥中ARF2与PLTs和PINs协调作用于ABA介导的根尖分生组织活性的调控

发布时间：2019-02-25 12:50

【摘要】：细胞的分裂和分化受到多种植物激素的调控。脱落酸(ABA)和生长素参与根生长和发育的调控,然而对其分子机制我们还知之甚少。之前的研究表明生长素响应因子2(ARF2)是ABA调控种子萌发和初生根生长通路中的负调控因子。PLT蛋白在控制根分生组织活性方面发挥重要作用。在本研究中,我们发现arf2突变体根生长对ABA超敏感的表型是由于ABA对静止中心细胞(QC)分裂的抑制以及对根尖分生区(RAM)细胞分裂和分化的抑制引起的。ABA通过ARF2降低PIN1, PIN3和PIN7在中柱的表达以及降低生长素在根尖分生区的积累。然而,在arf2突变体中,QC和柱细胞中高浓度的生长素抑制其分裂,而根尖分生区中低浓度的生长素抑制分生区细胞的分裂和伸长。与野生型相比,ABA处理减少了PIN1, PIN3, PIN7的表达,却增加了PIN4在arf2-101根尖中的积累。PIN4的突变可以部分逆转ABA对口arf2-101根生长的抑制。此外,在根尖分生区,ARF2在转录水平上正调控ABA所介导的PLT1的表达,但可能在转录后水平上间接调控PLT2。与arf2-101、pltl-4、plt2-2和plt1-4plt2-2突变体相比,arf2-101plt1-4双突对ABA更为敏感,分生区细胞数目更少。然而,利用DEX诱导型的转基因株系Pro35S:PLT2-GR过表达PLT2,我们发现PLT2在很大程度上促进细胞分裂,并且完全阻断arf2-101根分生组织的细胞分化,而这一过程可以被外源施加的ABA部分抑制。这表明ABA可通过ARF2依赖和不依赖的方式调控根的分生区。我们的结果揭示了拟南芥中ARF2与PLTs及PINs蛋白协同作用ABA介导的根分生组织调控的复杂网络。
[Abstract]:Cell division and differentiation are regulated by many plant hormones. Abscisic acid (ABA) and auxin are involved in the regulation of root growth and development, but little is known about its molecular mechanism. Previous studies have shown that auxin response factor 2 (ARF2) is a negative regulatory factor in seed germination and primary rooting growth pathway regulated by ABA. PLT protein plays an important role in controlling the activity of root meristem. In this study, we found that the phenotype of arf2 mutant root growth to ABA hypersensitivity was due to the inhibition of (QC) division by ABA and the inhibition of (RAM) cell division and differentiation in apical meristem region. ABA reduced PIN1, through ARF2. The expression of PIN3 and PIN7 in the middle column and the decrease of auxin accumulation in the apical meristem. However, in arf2 mutants, high concentrations of auxin in QC and columnar cells inhibited its division, while low concentrations of auxin in the apical meristem inhibited the division and elongation of meristem cells. Compared with wild type, ABA treatment decreased the expression of PIN1, PIN3, PIN7, but increased the accumulation of PIN4 in arf2-101 root tip. The mutation of PIN4 partially reversed the inhibition of ABA on arf2-101 root growth. In addition, at the apical meristem region, ARF2 is regulating the expression of PLT1 mediated by ABA at the transcriptional level, but may indirectly regulate PLT2. at the posttranscriptional level. Compared with arf2-101,pltl-4,plt2-2 and plt1-4plt2-2 mutants, arf2-101plt1-4 double processes are more sensitive to ABA and have less number of meristematic cells. However, using DEX inducible transgenic strain Pro35S:PLT2-GR to overexpression PLT2, we found that PLT2 promoted cell division to a great extent and completely blocked the differentiation of arf2-101 root meristem. This process can be partially inhibited by exogenous ABA. This indicates that ABA can regulate the meristematic region of root by ARF2 dependent and independent. Our results reveal a complex network of ABA-mediated root meristem regulation mediated by ARF2, PLTs and PINs proteins in Arabidopsis thaliana.
【学位授予单位】：中国农业大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：Q943.2

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