外源褪黑素对酸雨胁迫下番茄（Solanum lycopersicum L.）生长发育和转录组的影响研究
发布时间：2024-05-31 02:57
酸雨是全球性重大环境问题之一,因其污染可转移性受到广泛关注。长酸雨对植物的伤害机理一直是生态学研究的热点之一有证据表明酸雨对植物具有拮抗作用,尤其是对生长、光合活性、抗氧化活性和分子变化的影响。植物对酸雨的反应具有复杂的解毒和耐受的机制。然而,由于植物本身的抗氧化剂和解毒能力有限,在高浓度酸雨水平下常遭受毒害。因此,在这种情况下通过补充外源激素以加强植物解毒系统。褪黑激素(N-乙酰基-5-甲氧基色胺)是一种多功能信号分子,广泛分布于植物的不同部位,负责刺激几种生理反应应对不利环境条件。近年来,褪黑激素已成为植物激素类别的新范例,具有增强了植物对非生物胁迫因素的耐受性,包括热或冷的温度,盐度,干旱,涝害,紫外线辐射以及水和土壤中的化学或金属的污染物。因此,本研究旨在了解番茄植株对酸雨的胁迫响应,并通过一系列实验阐明褪黑素在缓解酸雨胁迫中的可能保护机制。重要发现总结如下:1.为了更好地了解植物胁迫响应酸雨的机理,探讨了两种不同水平的模拟酸雨(SAR)对两种番茄品种的光合作用、抗氧化酶活性以及非酶抗氧化物质含量的影响。结果表明,不同浓度的SAR水平,均显着降低了番茄品种叶片中植株生长,叶绿素...
【文章页数】：170 页
【学位级别】：博士
【文章目录】：
List of Abbreviations
摘要
Abstract
1 General introduction and literature review
1.1 Background of the study
1.2 Overview of acid rain stress and its consequence on plants
1.2.1 Causes and formation of acid rain
1.2.2 Problem of acid rain in plants
1.2.3 Impact of acid rain on plant growth
1.2.4 Impact of acid rain on photosynthesis activities in plants……
1.2.5 Impact of acid rain on antioxidant activities in plants
1.2.6 Impact of acid rain on molecular changes in plants
1.3 Melatonin and its overview in plants
1.3.1 History of melatonin in plants
1.3.2 Biosynthesis of melatonin in plants
1.3.3 Melatonin acts as a plant growth regulator
1.3.4 Melatonin acts as a bio-stimulator and an antioxidant in plant
1.3.5 Exogenous melatonin and plant stress tolerance
1.4 Melatonin in plant under acid rain condition
1.5 Problem statement and justification
1.6 General objectives
1.7 Specific objectives
1.8 Hypothesis
2 Acid rain deposition modulates photosynthesis,enzymatic and non-enzymatic antioxidant activities in tomato
2.1 Introduction
2.2 Materials and methods
2.2.1 Experimental site
2.2.2 Plant materials and treatments
2.2.3 Measurement of plant growth
2.2.4 Determination of chlorophyll and carotenoid content
2.2.5 Measurement of chlorophyll fluorescence
2.2.6 Estimation of enzymatic antioxidant compounds
2.2.7 Assay of hydrogen peroxide(H2O2)and lipid peroxide(MDA)content
2.2.8 Quantification of total soluble protein and total soluble sugar content
2.2.9 Determination of total phenolics and flavonoids content……
2.2.10 Estimation of total proline content
2.2.11 Measurement of reducing antioxidant activity
2.2.12 Statistical analysis
2.3 Results
2.3.1 Effects of simulated acid rain on growth
2.3.2 Effects of simulated acid rain on chlorophyll and carotenoid content
2.3.3 Effects of simulated acid rain on chlorophyll fluorescence….
2.3.4 Effects of simulated acid rain on enzymatic antioxidant activities
2.3.5 Effects of simulated acid rain on H2O2 and MDA
2.3.6 Effects of simulated acid rain on total soluble protein and soluble sugar
2.3.7 Effects of simulated acid rain on non-enzymatic antioxidant activities
2.4 Discussion
2.5 Conclusions
3 Exogenous melatonin mitigates acid rain stress to tomato plants through modulation of leaf ultrastructure,photosynthesis and antioxidant potential
3.1 Introduction
3.2 Materials and methods
3.2.1 Plant materials and treatments
3.2.2 Measurement of plant growth
3.2.3 Observation of leaf ultrastructure
3.2.4 Determination of photosynthesis activities
3.2.5 Assay of hydrogen peroxide(H2O2)and lipid peroxide(MDA)content
3.2.6 Estimation of enzymatic antioxidant compounds
3.2.7 Determination of non-enzymatic antioxidant compounds
3.2.8 Statistical analysis
3.3 Results
3.3.1 Effects of melatonin on growth of tomato plant under SAR stress
3.3.2 Effects of melatonin on leaf ultrastructure alterations in mesophyll cells and chloroplast of tomato under SAR stress..
3.3.3 Effects of melatonin on photosynthesis in tomato plant under SAR stress
3.3.4 Effects of melatonin on H2O2 and MDA content under SAR stress
3.3.5 Effect of melatonin on enzymatic antioxidant compounds under SAR stress
3.3.6 Effects of melatonin on nonenzymatic and total antioxidant activity under SAR stress
3.4 Discussion
3.5 Conclusion
4 Exogenous melatonin improves fruit quality features,health promoting antioxidant compounds and yield traits in tomato fruits under acid rain stress
4.1 Introduction
4.2 Materials and methods
4.2.1 Plant materials and treatments
4.2.2 Measurement of fruit quality
4.2.3 Estimation of enzymatic antioxidant activities,oxidative damage and total antioxidant power
4.2.4 Quantification of phenolics and flavonoids content
4.2.5 Estimation of carotenoids
4.2.6 Quantification of yield
4.2.7 Statistical Analysis
4.3 Results
4.3.1 Impact of exogenous melatonin on fruit qualities under SAR-stress condition
4.3.2 Impact of exogenous melatonin on antioxidant enzymes in fruits under SAR-stress condition
4.3.3 Impact of exogenous melatonin on oxidative damage and antioxidant potential in fruits under SAR-stress condition
4.3.4 Impact of exogenous melatonin on phenolics and flavonoids in fruits under SAR-stress condition
4.3.5 Impact of exogenous melatonin on carotenoids in fruits under SAR-stress condition
4.3.6 Impact of exogenous melatonin on yield attributes of tomatoes under SAR-stress condition
4.4 Discussion
4.5 Conclusions
5 Transcriptome analysis reveals the impact of exogenous melatonin on differential gene expression,biosynthesis of secondary metabolites and transcript factors in tomato under acid rain stress
5.1 Introduction
5.2 Materials and methods
5.2.1 Plant materials and treatments
5.2.2 RNA quality examination
5.2.3 Library preparation for mRNA-sequence
5.2.4 Library examination
5.2.5 Library clustering and sequencing
5.2.6 Transcriptome analysis
5.2.7 Quantitative Real-time PCR(qRT-PCR)method
5.2.8 Statistical analysis
5.3 Results
5.3.1 RNA quality test results
5.3.2 RNA-sequencing analysis
5.3.3 Differentially expressed genes(DEGs)
5.3.4 Mutual DEGs analysis
5.3.5 Gene ontology analysis of DEGs
5.3.6 KEGG pathway analysis of DEGs
5.3.7 Transcript factors
5.3.8 Confirmation of gene expression profile through qRT-PCR…
5.4 Discussion
5.5 Conclusions
6 General discussion,conclusions and future perspectives
6.1 General discussion
6.2 Conclusions
6.3 Future perspectives
References
Published papers
Acknowledgements
本文编号：3985043
【文章页数】：170 页
【学位级别】：博士
【文章目录】：
List of Abbreviations
摘要
Abstract
1 General introduction and literature review
1.1 Background of the study
1.2 Overview of acid rain stress and its consequence on plants
1.2.1 Causes and formation of acid rain
1.2.2 Problem of acid rain in plants
1.2.3 Impact of acid rain on plant growth
1.2.4 Impact of acid rain on photosynthesis activities in plants……
1.2.5 Impact of acid rain on antioxidant activities in plants
1.2.6 Impact of acid rain on molecular changes in plants
1.3 Melatonin and its overview in plants
1.3.1 History of melatonin in plants
1.3.2 Biosynthesis of melatonin in plants
1.3.3 Melatonin acts as a plant growth regulator
1.3.4 Melatonin acts as a bio-stimulator and an antioxidant in plant
1.3.5 Exogenous melatonin and plant stress tolerance
1.4 Melatonin in plant under acid rain condition
1.5 Problem statement and justification
1.6 General objectives
1.7 Specific objectives
1.8 Hypothesis
2 Acid rain deposition modulates photosynthesis,enzymatic and non-enzymatic antioxidant activities in tomato
2.1 Introduction
2.2 Materials and methods
2.2.1 Experimental site
2.2.2 Plant materials and treatments
2.2.3 Measurement of plant growth
2.2.4 Determination of chlorophyll and carotenoid content
2.2.5 Measurement of chlorophyll fluorescence
2.2.6 Estimation of enzymatic antioxidant compounds
2.2.7 Assay of hydrogen peroxide(H2O2)and lipid peroxide(MDA)content
2.2.8 Quantification of total soluble protein and total soluble sugar content
2.2.9 Determination of total phenolics and flavonoids content……
2.2.10 Estimation of total proline content
2.2.11 Measurement of reducing antioxidant activity
2.2.12 Statistical analysis
2.3 Results
2.3.1 Effects of simulated acid rain on growth
2.3.2 Effects of simulated acid rain on chlorophyll and carotenoid content
2.3.3 Effects of simulated acid rain on chlorophyll fluorescence….
2.3.4 Effects of simulated acid rain on enzymatic antioxidant activities
2.3.5 Effects of simulated acid rain on H2O2 and MDA
2.3.6 Effects of simulated acid rain on total soluble protein and soluble sugar
2.3.7 Effects of simulated acid rain on non-enzymatic antioxidant activities
2.4 Discussion
2.5 Conclusions
3 Exogenous melatonin mitigates acid rain stress to tomato plants through modulation of leaf ultrastructure,photosynthesis and antioxidant potential
3.1 Introduction
3.2 Materials and methods
3.2.1 Plant materials and treatments
3.2.2 Measurement of plant growth
3.2.3 Observation of leaf ultrastructure
3.2.4 Determination of photosynthesis activities
3.2.5 Assay of hydrogen peroxide(H2O2)and lipid peroxide(MDA)content
3.2.6 Estimation of enzymatic antioxidant compounds
3.2.7 Determination of non-enzymatic antioxidant compounds
3.2.8 Statistical analysis
3.3 Results
3.3.1 Effects of melatonin on growth of tomato plant under SAR stress
3.3.2 Effects of melatonin on leaf ultrastructure alterations in mesophyll cells and chloroplast of tomato under SAR stress..
3.3.3 Effects of melatonin on photosynthesis in tomato plant under SAR stress
3.3.4 Effects of melatonin on H2O2 and MDA content under SAR stress
3.3.5 Effect of melatonin on enzymatic antioxidant compounds under SAR stress
3.3.6 Effects of melatonin on nonenzymatic and total antioxidant activity under SAR stress
3.4 Discussion
3.5 Conclusion
4 Exogenous melatonin improves fruit quality features,health promoting antioxidant compounds and yield traits in tomato fruits under acid rain stress
4.1 Introduction
4.2 Materials and methods
4.2.1 Plant materials and treatments
4.2.2 Measurement of fruit quality
4.2.3 Estimation of enzymatic antioxidant activities,oxidative damage and total antioxidant power
4.2.4 Quantification of phenolics and flavonoids content
4.2.5 Estimation of carotenoids
4.2.6 Quantification of yield
4.2.7 Statistical Analysis
4.3 Results
4.3.1 Impact of exogenous melatonin on fruit qualities under SAR-stress condition
4.3.2 Impact of exogenous melatonin on antioxidant enzymes in fruits under SAR-stress condition
4.3.3 Impact of exogenous melatonin on oxidative damage and antioxidant potential in fruits under SAR-stress condition
4.3.4 Impact of exogenous melatonin on phenolics and flavonoids in fruits under SAR-stress condition
4.3.5 Impact of exogenous melatonin on carotenoids in fruits under SAR-stress condition
4.3.6 Impact of exogenous melatonin on yield attributes of tomatoes under SAR-stress condition
4.4 Discussion
4.5 Conclusions
5 Transcriptome analysis reveals the impact of exogenous melatonin on differential gene expression,biosynthesis of secondary metabolites and transcript factors in tomato under acid rain stress
5.1 Introduction
5.2 Materials and methods
5.2.1 Plant materials and treatments
5.2.2 RNA quality examination
5.2.3 Library preparation for mRNA-sequence
5.2.4 Library examination
5.2.5 Library clustering and sequencing
5.2.6 Transcriptome analysis
5.2.7 Quantitative Real-time PCR(qRT-PCR)method
5.2.8 Statistical analysis
5.3 Results
5.3.1 RNA quality test results
5.3.2 RNA-sequencing analysis
5.3.3 Differentially expressed genes(DEGs)
5.3.4 Mutual DEGs analysis
5.3.5 Gene ontology analysis of DEGs
5.3.6 KEGG pathway analysis of DEGs
5.3.7 Transcript factors
5.3.8 Confirmation of gene expression profile through qRT-PCR…
5.4 Discussion
5.5 Conclusions
6 General discussion,conclusions and future perspectives
6.1 General discussion
6.2 Conclusions
6.3 Future perspectives
References
Published papers
Acknowledgements
本文编号：3985043
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