石菖蒲对抑郁模型大鼠海马组织mTOR信号通路相关蛋白表达的影响

发布时间：2017-12-27 09:16

本文关键词：石菖蒲对抑郁模型大鼠海马组织mTOR信号通路相关蛋白表达的影响　出处：《中国临床医学》2016年05期 　论文类型：期刊论文

【摘要】：目的:探讨石菖蒲对抑郁模型大鼠海马组织mTOR信号通路相关蛋白表达的影响。方法:采用孤养结合慢性不可预见应激方法造成大鼠抑郁,在7、14、21、28d时,检测各组大鼠旷场实验中水平运动、垂直运动得分,并用Western印迹法检测各组大鼠海马组织mTOR信号通路相关蛋白的表达变化。结果:21d、28d时,与对照组比较,模型组垂直运动和水平运动得分减少(P0.01);与模型组比较,中、西药组垂直运动和水平运动得分增加(P0.05、0.01)。21d、28d时,与对照组比较,模型组大鼠海马Akt、mTOR、p70S6K和elF-4E蛋白的表达下调(P0.01);与模型组比较,中、西药组大鼠海马Akt、mTOR、p70S6K和elF-4E蛋白表达增加(P0.01)。结论:石菖蒲的抗抑郁效应可能通过激活mTOR信号通路相关蛋白的表达而实现。
[Abstract]:Objective: To investigate the effect of Acorus calamus on the expression of mTOR signaling pathway related protein in the hippocampus of depression model rats. Methods: the solitary with chronic unpredictable stress rats caused by depression, in 7, 14, 21, 28d, detection of horizontal motion and open field experiments in rats and the expression of vertical motion score by Western blotting in the hippocampus of rats mTOR signaling pathway related protein. Results: at 21d and 28d, compared with the control group, the scores of vertical and horizontal movements in model group decreased (P0.01), and the scores of vertical and horizontal movements increased in Chinese and Western medicine group (P0.05, 0.01). Compared with the control group, the expression of Akt, mTOR, p70S6K and elF-4E proteins in the hippocampus of the model group was decreased (P0.01) when compared with the control group (P0.01). Compared with the model group, the expression of Akt, mTOR, p70S6K and elF-4E protein increased in the hippocampus of the Chinese and Western medicine group (28d). (21d) Conclusion: the antidepressant effect of Acorus calamus may be realized by activating the expression of mTOR signaling pathway related proteins.
【作者单位】：上海市杨浦区精神卫生中心;
【分类号】：R285.5;R-332
【正文快照】： 抑郁症是一种常见的心境障碍,以情绪低落、兴趣丧失等为主要临床表现[1]。孤养结合慢性不可预知性应激能造成较理想的抑郁状态[2],是目前最好的抑郁症造模方式[3]。mTOR是一种非典型丝氨酸/苏氨酸蛋白激酶,其作用是调控蛋白质翻译起始阶段。近期研究[4]发现,通过激活mTOR信号

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