结核分枝杆菌panD蛋白功能预测及生物信息学分析

发布时间：2017-12-27 10:10

  本文关键词：结核分枝杆菌panD蛋白功能预测及生物信息学分析　出处：《中国病原生物学杂志》2017年06期 　论文类型：期刊论文

  更多相关文章： 结核分枝杆菌 panD 抗原表位 耐药 生物信息学

【摘要】：目的研究结核分枝杆菌panD(Rv3601c)基因及其编码蛋白的结构和功能,为耐药结核的治疗提供参考依据。方法运用ClustalX 2.1、MEGA 6.06软件以及ExPASy、NCBI等在线工具分析结核分枝杆菌panD基因信息,同时分析其进化特征、编码蛋白质的理化性质、信号肽、磷酸化位点、结构特点并预测其功能;利用BepiPred 1.0Server和NetMHCIIpan 3.1Server预测panD蛋白的B细胞和T细胞抗原表位。结果结核分枝杆菌panD基因全长420bp,不同菌株panD基因序列相似度100%,具有高度同源性,进化关系较近,编码139个氨基酸。panD蛋白不稳定系数为8.72,亲水性平均系数为0.147,为稳定、疏水性蛋白、无跨膜区与信号肽,存在9个磷酸化位点。二级结构中以无规则卷曲为主,结构较疏松。三级结构同源建模成功。该蛋白具有多个潜在的B细胞抗原表位和T细胞抗原表位。结论panD蛋白作为脱羧酶,在结核分枝杆菌合成β-丙氨酸过程中具有重要作用。panD蛋白序列保守稳定,具有多个优势抗原表位,是治疗耐药结核的潜在新靶标。
[Abstract]:Objective to study the structure and function of Mycobacterium tuberculosis panD (Rv3601c) gene and its encoded protein, so as to provide reference for the treatment of drug-resistant tuberculosis. Methods using ClustalX 2.1 and MEGA 6.06 software and ExPASy, NCBI and other online tools to analyze the genetic information of Mycobacterium tuberculosis panD, and analyzes its evolution characteristics, encoding protein physicochemical properties, signal peptide, phosphorylation sites, the structure characteristics and predict its function; prediction of panD protein by BepiPred 1.0Server and NetMHCIIpan 3.1Server and T B cells cell epitope. Results the panD gene of Mycobacterium tuberculosis was 420bp, and the sequence similarity of different panD genes was 100%, which was highly homologous. The evolutionary relationship was close, encoding 139 amino acids. The panD protein instability coefficient was 8.72, the average hydrophilic coefficient was 0.147, which was stable, hydrophobic protein, transmembrane free zone and signal peptide, and there were 9 phosphorylation sites. The two stage structure is dominated by irregular curling, and the structure is loose. Three level structure homologous modeling is successful. The protein has multiple potential B cell epitopes and T cell epitopes. Conclusion panD protein, as decarboxylase, plays an important role in the synthesis of beta - alanine in Mycobacterium tuberculosis. The panD protein sequence is conservative and stable, and has multiple dominant epitopes. It is a potential new target for the treatment of drug-resistant tuberculosis.
【作者单位】： 济宁医学院临床医学院;潍坊医学院;潍坊医学院临床医学院;
【基金】：国家自然科学基金项目(No.30972639) 山东省自然科学基金项目(No.ZR2016HM09)
【分类号】：R378.911
【正文快照】： 结核病是由胞内寄生菌-结核分枝杆菌(Mycobac-terium tuberculosis,MTB)引起的传染病。随着近几年抗生素的滥用和潜伏感染、耐药结核的出现,使结核***病的根治面临重重困难。我国是全球22个结核病高负担国家之一,近年来MTB耐药、耐多药情况尤为严重[1]。吡嗪酰胺(pyrazinamide，

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