抵抗素上调脂肪酸转位酶表达对HepG2细胞脂质积聚的作用

发布时间：2017-12-27 23:27

本文关键词：抵抗素上调脂肪酸转位酶表达对HepG2细胞脂质积聚的作用　出处：《中国老年学杂志》2017年16期 　论文类型：期刊论文

【摘要】：目的探讨抵抗素在棕榈酸诱导下对HepG2肝细胞脂质积聚的作用及其对脂肪酸转位酶(FAT/CD36)表达调控的影响。方法 50 ng/ml重组人抵抗素及0.5 mmol/L棕榈酸孵育HepG2肝细胞,之后用100 nmol/L胰岛素处理,采用实时定量RT-PCR检测胆固醇调节元件结合蛋白(SREBP)1 mRNA水平,流式细胞仪检测胞膜CD36表达,尼罗红(Nile Red)染色后激光共聚焦显微镜检测胞内脂质含量。结果与对照组相比,抵抗素增加胞膜FAT/CD36含量(P0.05),促进HepG2细胞SREBP1 mRNA表达(P0.05),使胞内红色脂肪小滴增多(P0.05)。结论在高浓度游离饱和脂肪酸环境中,抵抗素在基础及胰岛素刺激状态下通过上调HepG2的CD36表达,刺激SREBP1转录,导致HepG2肝细胞内脂质积聚。
[Abstract]:Objective to investigate the effect of resistin on the accumulation of lipid in HepG2 liver cells induced by palmitic acid and its effect on the regulation of the expression of fatty acid transposition enzyme (FAT/CD36). Methods 50 ng/ml recombinant human resistin and 0.5 mmol/L palmitic acid were incubated in HepG2 liver cells, after treatment with 100 nmol/L insulin, cholesterol was detected by real-time quantitative RT-PCR regulatory element binding protein (SREBP) level 1 mRNA, detect the expression of CD36 in cell membrane by flow cytometry, Nile red (Nile Red) after staining with confocal laser lipid content microscopy and detection of intracellular. Results compared with the control group, resistin increased the content of FAT/CD36 membrane (P0.05), SREBP1 mRNA cells promote the expression of HepG2 (P0.05), the intracellular red fat droplets increased (P0.05). Conclusion in a high concentration of free fatty acid environment, resistin stimulates SREBP1 transcription by up regulating the expression of HepG2 CD36 in basal and insulin stimulated state, resulting in lipid accumulation in HepG2 hepatocytes.
【作者单位】：中山大学孙逸仙纪念医院检验科;中山大学孙逸仙纪念医院内分泌科;
【基金】：国家自然科学基金资助项目(30570886) 教育部留学回国人员科研启动基金资助项目(教外司留[2015]1098号) 广东省自然科学基金资助项目(2016A030313345)
【分类号】：R3416
【正文快照】： 脂毒性是指血中游离脂肪酸(FFA)水平升高后,超过脂肪组织的储存能力和各组织对FFA的氧化能力,使过多的FFA以甘油三酯(TG)等形式在非脂肪组织中过度沉积而造成对该组织的损伤。FFA与胰岛素抵抗(IR)密切相关,脂肪的异位沉积已被认为是形成IR的重要因素,而肝脏是最易也是较早发生

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