细粒棘球绦虫myophilin的抗原表位预测

发布时间：2017-12-28 14:33

本文关键词：细粒棘球绦虫myophilin的抗原表位预测　出处：《中国病原生物学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的应用生物信息学软件预测细粒棘球绦虫亲肌肉蛋白myophilin的二级结构及B、T细胞抗原表位,为其保护性抗原表位筛选提供依据。方法从Genbank中获取myophilin的氨基酸序列,采用SOPMA软件预测其二级结构;采用LEPS和ABCpred软件联合预测其B细胞抗原表位;采用IEDB和SYFPEITHI软件联合预测其T细胞抗原表位。结果α螺旋、β折叠、无规则卷曲和β转角在myophilin二级结构中所占的比例分别为47.89%、10.00%、30.53%和11.58%;潜在的B细胞抗原表位分别为19~27、41~48、92~101、110~118、128~137和155~162;潜在的T细胞抗原表位为9~18、140~148和157~165氨基酸序列。结论 myophilin蛋白可能有6个B细胞抗原表位和3个T细胞抗原表位,有望用作免疫诊断抗原和疫苗候选抗原。
[Abstract]:Objective bioinformatics software was applied to predict the two level structure of the muscle protein myophilin of Echinococcus granulosus and the epitope of B and T cells, so as to provide evidence for screening the protective epitopes of Echinococcus granulosus. Methods the amino acid sequences of myophilin were obtained from Genbank. The secondary structure was predicted by SOPMA software. The B cell epitopes were predicted by LEPS and ABCpred software. The T cell epitopes were predicted by IEDB and SYFPEITHI software. The alpha helix and beta folding, random coil and beta angle for myophilin two level structure in the proportion was 47.89%, 10%, 30.53% and 11.58%; the potential B cell epitopes were 19~27, 41~48, 92~101, 110~118, 128~137 and 155~162; the potential T cell epitopes, 9~18 140~148 and 157~165 amino acid sequence. Conclusion myophilin protein may have 6 B cell antigen epitopes and 3 T cell antigen epitopes, which are expected to be used as immuno diagnostic antigen and vaccine candidate antigen.
【作者单位】：青海大学生态环境工程学院;青海大学医学院基础医学研究中心;
【基金】：国家自然科学基金项目(No.31260015) 青海省科技厅项目(No.2016-ZJ-746)
【分类号】：R392
【正文快照】： 棘球蚴感染人体可引起包虫病,该病在我国西北畜牧地区广泛流行,对当地居民健康和畜牧业发展危害极其严重[1]。可靠的诊断方法和有效的预防措施对该病的防控至关重要,其中免疫诊断和疫苗预防是一种切实可行的途径[2]。亲肌肉蛋白(myophilin)是棘球绦虫的一种天然抗原,有较强的

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