建立改良法兔VX2肝癌模型及肝动脉联合门静脉插管的应用

发布时间：2017-12-30 17:46

本文关键词：建立改良法兔VX2肝癌模型及肝动脉联合门静脉插管的应用　出处：《中国介入影像与治疗学》2017年11期 　论文类型：期刊论文

更多相关文章：兔 VX肝癌 放射学 介入性 回归分析

【摘要】：目的采用改良法建立兔VX2肝癌模型,探讨经肝动脉联合门静脉插管技术的应用并分析其影响因素。方法对60只健康新西兰大白兔行改良开腹穿刺接种法移植肝癌,并行肝动脉联合门静脉造影,根据术后是否发生短期死亡(术后7天内)分为存活组和死亡组,将可能导致实验兔死亡的因素进行单因素分析,以有统计学意义的指标为自变量,建立二分类Logistic回归模型,分析导致实验兔死亡的独立危险因素。结果 60只实验兔均移植肿瘤成功,9只(9/60,15.00%)实验兔术后短期内死亡,51只(51/60,85.00%)存活。单因素分析显示两组间体质量2.5kg、术中追加麻醉、手术时间≥60min、切口长度≥5cm及总出血量≥25ml为相关影响因素(P均0.05)。Logistic回归分析筛选出体质量2.5kg、术中追加麻醉及出血量≥25ml为手术死亡的独立危险因素(P均0.01)。结论改良法兔VX2肝癌模型建立成功率较高;选择体重较大的实验兔,同时加强手术技巧的训练,减少术中出血量和避免术中追加麻醉是进行肝动脉联合门静脉造影时降低实验兔死亡率的有效措施。
[Abstract]:Objective to establish rabbit VX2 liver cancer model by modified method to evaluate the hepatic artery and portal vein intubation technique and analyze its influencing factors. Method of transplant liver cancer in 60 healthy New Zealand white rabbits underwent laparotomy stabinoculation, hepatic artery combined with portal vein angiography, according to whether there was postoperative short-term mortality (7 days after operation inside) divided into survival group and death group, will likely lead to death factors of experimental rabbits were analyzed by univariate analysis, with statistically significant indicators as independent variables, the establishment of two classification Logistic regression model, analysis of risk factors causing death in rabbits. The results of 60 experimental rabbits were successfully transplanted tumor, 9 (9/60,15.00%) the rabbits died in a short period after the operation, only 51 (51/60,85.00%). Univariate analysis showed that the survival between the two groups in body weight 2.5kg, intraoperative additional anesthesia, operation time is larger than 60min, the length of incision was 5cm and the total amount of bleeding is more than 25ml related factors (P 0.05) analysis showed that the body weight of 2.5kg.Logistic regression, intraoperative additional anesthesia and bleeding more than 25ml independent risk factors of operative death (P 0.01). Conclusion the improved method of establishing rabbit VX2 liver cancer model has higher success rate; weight larger experimental rabbits, while strengthening the operation skills training, reducing the amount of intraoperative bleeding and avoid intraoperative additional anesthesia are effective measures to reduce the mortality of rabbit hepatic artery combined with portal vein angiography.

【作者单位】：合肥市第三人民医院影像中心;
【分类号】：R-332;R735.7
【正文快照】： 经导管动脉化疗栓塞术(transcatheter　arterialchemoembolization,TACE)已成为中晚期原发性肝癌的标准治疗方式[1],但单纯TACE具有短期复发,且复发率较高的缺点,肝癌存在肝动脉和门静脉双重血供是其复发的主要原因。研究[2-4]发现采用经肝动脉联合门静脉化疗栓塞术治疗中晚期

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