左乙拉西坦和甘珀酸对癫痫模型大鼠改善作用的比较及相关分子机制研究

发布时间：2017-12-31 09:21

本文关键词：左乙拉西坦和甘珀酸对癫痫模型大鼠改善作用的比较及相关分子机制研究　出处：《海南医学院学报》2016年19期 　论文类型：期刊论文

【摘要】：目的:研究左乙拉西坦和甘珀酸对癫痫模型大鼠癫痫发作情况、神经元凋亡情况以及缝隙连接蛋白表达情况的影响。方法:选择雄性SD大鼠并分为对照组、癫痫模型组、甘珀酸组、左乙拉西坦组,癫痫模型组、甘珀酸组、左乙拉西坦组采用氯化锂-匹罗卡品腹腔注射的方式建立癫痫模型,并分别给予生理盐水、甘珀酸、左乙拉西坦治疗,观察4组大鼠癫痫发作情况并测定大脑海马组织中细胞凋亡情况以及Cx26、Cx30、Cx43、Bcl-2、Bax、Caspase-3的表达量。结果:左乙拉西坦组和甘珀酸组大鼠癫痫发作潜伏期比癫痫模型组显著延长、发作次数显著少于癫痫模型组(P0.05),左乙拉西坦组大鼠癫痫发作潜伏期比甘珀酸组显著延长、发作次数显著少于甘珀酸组(P0.05)。左乙拉西坦组和甘珀酸组大鼠海马组织中Cx26、Cx30、Cx43、Bax、Caspase-3的蛋白含量显著低于癫痫模型组(P0.05),Bcl-2的蛋白含量显著高于癫痫模型组(P0.05),TUNNEL阳性染色细胞数目显著少于癫痫模型组(P0.05),Nissl阳性染色细胞数目显著多于癫痫模型组(P0.05);左乙拉西坦组大鼠海马组织中Cx26、Cx30、Cx43、Bax、Caspase-3的蛋白含量显著低于甘珀酸组(P0.05),Bcl-2的蛋白含量显著高于甘珀酸组(P0.05),TUNNEL阳性染色细胞数目显著少于甘珀酸组(P0.05),Nissl阳性染色细胞数目显著多于甘珀酸组(P0.05)。结论:左乙拉西坦抑制癫痫模型大鼠癫痫发作的作用优于甘珀酸,抑制缝隙连接蛋白表达以及细胞凋亡是左乙拉西坦发挥抗癫痫作用的分子机制。
[Abstract]:Objective: to study the effects of levoethylacetam and glycyrrhizic acid on seizure, neuronal apoptosis and gap junction protein expression in epileptic model rats. Methods: male SD rats were divided into two groups. The epileptic model group, carbenic acid group, levoethylacetam group, epileptic model group, glycyrbic acid group and levoethylacetam group were injected intraperitoneally with lithium-pilocarpine to establish the epileptic model, and were given normal saline respectively. The seizure of rats in 4 groups were observed and apoptosis in hippocampus and Bax of Cx26Cx30 Cx43 and Bcl-2BX were measured. Results: the latent period of epileptic seizures in levoethylacetam group and glycyrrhizic acid group was significantly longer than that in epileptic model group, and the number of seizures was significantly less than that in epileptic model group (P0.05). The latency of epileptic seizures in levoethylacetam group was significantly longer than that in glycyrbic acid group, and the number of seizures was significantly less than that in carbenic acid group (P 0.05). The Cx26 in hippocampus of rats in levoethylacetam group and glyoxetam group was significantly lower than that in carbenic acid group. The content of Caspase-3 in Cx30 Cx43 was significantly lower than that in epileptic model group (P0.05). The protein content of Bcl-2 was significantly higher than that of P0.05TUNNEL positive cells in epileptic model group (P 0.05). The number of Nissl positive staining cells was significantly higher than that of epileptic model group (P 0.05). The protein content of Cx26, Cx30, Cx43 and BaxCaspase-3 in rat hippocampus of levoethylacetam group was significantly lower than that of glycyrrhizic acid group (P0.05). The protein content of Bcl-2 was significantly higher than that of carbenc acid group, and the number of TUNNEL positive cells was significantly lower than that of carbenic acid group (P0.05). The number of Nissl positive staining cells was significantly higher than that of carbenic acid group (P 0.05). Conclusion: the inhibitory effect of levoethylacetam on epileptic seizures in epileptic model rats was superior to that of glycyrrhizic acid. Inhibition of gap junction protein expression and apoptosis are the molecular mechanisms of antiepileptic effect of levoethylacetam.
【作者单位】：陕西省西安高新医院神经内科;
【基金】：陕西省自然科学发展项目(XZ-421G)~~
【分类号】：R742.1;R-332
【正文快照】： Epileptic seizure incubation of levetiracetam group and carbenoxolone group were significantly longer than that of epilepsy mod-el group and the frequency of seizures were significantly less than that of epilepsy model group,epileptic seizure incubation

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