缬草醛对肠易激综合征模型大鼠结肠CRF,TPH1 mRNA及5-HT表达的影响

发布时间：2017-12-31 15:12

本文关键词：缬草醛对肠易激综合征模型大鼠结肠CRF,TPH1 mRNA及5-HT表达的影响　出处：《中国中药杂志》2017年02期 　论文类型：期刊论文

【摘要】：该研究旨在通过探讨蜘蛛香环烯醚萜类成分缬草醛对肠易激综合征(IBS)大鼠结肠中促肾上腺皮质激素释放因子(CRF)、色氨酸羟化酶1(TPH1)mRNA表达及5-羟色胺(5-HT)含量的影响,阐释缬草醛通过5-HT通路治疗IBS的作用机制。将54只SD雄性大鼠随机分为空白对照组、模型组、缬草醛高、中、低剂量组、匹维溴铵组,每组9只。采用孤养结合慢-急性不可预见性应激法建立IBS大鼠模型,免疫组化法测定大鼠结肠CRF表达,RT-PCR法测定结肠中TPH1 mRNA表达,高效液相色谱法测定大鼠结肠中5-HT含量。实验结果显示,孤养结合慢-急性不可预见性应激可造成大鼠结肠中CRF,TPH1 mRNA表达以及5-HT含量升高(P0.05),缬草醛各剂量组中CRF,TPH1 mRNA及5-HT含量较模型组明显下降(P0.05)。实验结果表明,肠易激综合征能够导致结肠中CRF,TPH1 mRNA的表达以及5-HT的含量升高,而缬草醛通过降低结肠中CRF,TPH1 mRNA的表达以及5-HT的含量,而改善胃肠道的相关症状。
[Abstract]:The aim of this study was to investigate the iridoids composition baldrinal on irritable bowel syndrome (IBS) in colon of rat corticotropin releasing factor (CRF), tryptophan hydroxylase 1 (TPH1) mRNA expression of 5- and serotonin (5-HT) on the content of interpretation by baldrinal mechanism the 5-HT pathway for the treatment of IBS. 54 male SD rats were randomly divided into control group, model group, baldrinal high, low dose group, pinaverium bromide group, 9 rats in each group. The combination of slow - solitary acute can establish the rat model of IBS foresight stress method, immunohistochemistry method for the determination of CRF expression in rat colon and to determine the expression of TPH1 mRNA in colon RT-PCR method and HPLC method for the determination of the content of 5-HT in rat colon. The experimental results show that the combined solitary slow - acute unpredictable stress can cause rat colon CRF, mRNA expression of TPH1 and 5-HT containing Liang Shenggao (P0.05). Baldrinal Each dose group of CRF, TPH1, mRNA and 5-HT content decreased significantly compared with model group (P0.05). The experimental results show that the irritable bowel syndrome can lead to colon in CRF, TPH1 increased the expression of mRNA and 5-HT content, and baldrinal by reducing the colon CRF, TPH1 mRNA expression and 5-HT content, and improve the symptoms related to the gastrointestinal tract.

【作者单位】：北京中医药大学基础医学院;北京中医药大学中药学院;
【基金】：国家自然科学基金项目(81374044) 校级自主课题项目(2016-JYB-XS028)
【分类号】：R285.5;R-332
【正文快照】： 2.北京中医药大学中药学院,北京100102)肠易激综合征(irritable bowel syndrome,IBS)是一种临床常见的胃肠功能紊乱性疾病,以腹痛、腹泻、排便习惯和排便性状异常为主要症状,持续存在或间歇发作,而无形态学和生化异常改变的症候群[1]。IBS发病机制尚未完全明确,目前普遍认为内

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