脾气虚证模型大鼠尿液的~1H-NMR代谢组学研究

发布时间：2017-12-31 19:37

本文关键词：脾气虚证模型大鼠尿液的~1H-NMR代谢组学研究　出处：《中国药理学通报》2017年10期 　论文类型：期刊论文

【摘要】：目的建立脾气虚证大鼠模型,分析脾气虚证的代谢通路,探究内源性代谢产物变化与脾气虚证的关系。方法采用苦寒泻下、劳倦过度和饥饱失常复合因素方法建立并评价脾气虚证大鼠模型,检测其血清中肌酸磷酸激酶活性;对大鼠尿液进行~1H-NMR检测,以变化倍数(fold change,FC)1.2,结合独立样本t检验(P0.05)的统计学方法筛选脾气虚证模型组和对照组的差异代谢物,进行代谢通路分析与富集分析。结果脾气虚证模型建立成功;模型组的肌酸磷酸激酶活性低于对照组(P0.05);从主成分分析(PCA)结果得出模型组和对照组的代谢产物得到明显区分;筛选出33种差异代谢物,主要参与的代谢通路涉及能量代谢、氨基酸代谢、核苷酸代谢,同时对肠道菌群存在干扰。结论脾气虚主要扰乱了大鼠的能量代谢通路(三羧酸循环、糖酵解、脂质氧化),抑制了供能作用,导致大鼠出现疲乏和体质量增长抑制的症状。
[Abstract]:Objective to establish the model of spleen deficiency rats, metabolic pathway analysis of spleen qi deficiency, spleen deficiency syndrome and explore the relationship between changes of endogenous metabolites. Using the method of cold diarrhea, overstrain and hunger to establish evaluation model and method of complex factors of arrhythmia in spleen qi deficiency rats, detection of creatine kinase activity in serum; ~1H-NMR was detected in rat urine changes in the ratio (fold, change, FC) 1.2, with independent samples t test (P0.05) statistical methods for differential screening of spleen qi deficiency model group and control group of metabolites, metabolic pathway analysis and enrichment analysis. The results of spleen deficiency syndrome model; model group of creatine kinase activity was lower than that of control group (P0.05); analysis the principal component (PCA) results of metabolites in the model group and the control group were significantly difference; identified 33 different metabolites and metabolic pathways to participate to Energy metabolism, amino acid metabolism, nucleotide metabolism, and the intestinal flora disturbance. Conclusion spleen deficiency mainly disrupted energy metabolism in rats (three carboxylic acid cycle, glycolysis, lipid oxidation), inhibited the energy supply, resulting in the rats with fatigue and body weight growth inhibition of the symptoms.

【作者单位】：广州中医药大学中药学院;无限极(中国)有限公司;中山大学药学院;
【基金】：国家自然科学基金资助项目(No 81473540,81473319) 广东省科技计划项目(No 2014A020221027,2013B090800011,2014B040404010,2015A030401031) 广东省自然科学基金资助项目(No 015A030313123)
【分类号】：R228;R-332
【正文快照】： 脾气虚证是临床常见的一种证型,主要表现为脾气不足,运化失职所致的虚弱症状。近年来,关于脾气虚本质的基础研究多集中于消化功能、能量代谢和免疫功能等方面[1],不少学者致力于脾气虚证模型的生化指标研究,但是对于其代谢通路与病证之间的关系,我们知之甚少。代谢组学(metabo

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