瞬时感受电位香草酸亚家族蛋白1受体在慢性偏头痛大鼠模型中的作用研究

发布时间：2018-01-01 02:20

本文关键词：瞬时感受电位香草酸亚家族蛋白1受体在慢性偏头痛大鼠模型中的作用研究　出处：《中风与神经疾病杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的本研究通过炎性汤(IS)反复刺激SD大鼠上矢状窦区硬脑膜建立慢性偏头痛(CM)大鼠模型,探讨瞬时感受电位香草酸亚家族蛋白1(TRPV1)受体在CM发病过程中的作用,为研究CM发病机制和治疗药物提供理论依据。方法 72只SD大鼠随机数字表法分为空白对照组(A组)、假手术组(B组)、CM模型组(C组)及TRPV1受体拮抗剂Capsazepine组(D组),采用免疫组织化学染色、Western-Blot、Real-time PCR技术检测大鼠硬脑膜、三叉神经节(TG)、三叉神经脊束尾侧核(TNC)中的TRPV1受体、降钙素基因相关肽(CGRP)表达量变化。结果 TRPV1受体和CGRP在CM大鼠硬脑膜、TG及TNC上的表达量均增加(P0.05),通过侧脑室注射Capsazepine药物后明显缓解大鼠疼痛,且TRPV1受体、CGRP表达量均明显下降(P0.05)。结论 TRPV1受体通过影响CGRP释放参与CM神经源性炎症反应及痛觉传导,提示TRPV1可能通过TRPV1-CGRP信号通路参与CM病理生理过程。
[Abstract]:The purpose of this study is through the inflammatory Decoction (IS) repeated stimulation of SD rats of succinylcholine established chronic migraine (CM) rat model of transient receptor potential vanilloid subfamily protein 1 (TRPV1) receptors in the pathogenesis of CM, and provide a theoretical basis for the research and treatment of drug CM disease the mechanism. Methods 72 SD rats were randomly divided into control group (group A), sham operation group (B group), CM model group (group C) and TRPV1 receptor antagonist Capsazepine group (group D), immunohistochemical staining, Western-Blot, detection of Real-time PCR in the dura (TG), trigeminal ganglion, spinal trigeminal caudal nucleus (TNC) of the TRPV1 receptor, calcitonin gene related peptide (CGRP) expression of TRPV1 receptor and CGRP. Results in the dural CM rats. The expression of TG and TNC increased (P0.05), by intracerebroventricular injection of Capsazepine the drug was slow The pain and the expression of TRPV1 receptor and CGRP decreased significantly (P0.05). Conclusion TRPV1 receptor is involved in CM neurogenic inflammation and pain conduction through the influence of CGRP release, suggesting TRPV1 may participate in CM pathophysiology through TRPV1-CGRP signaling pathway.

【作者单位】：滨州医学院附属医院神经内科;滨州医学院附属医院神经内科一病区护理部;
【基金】：山东省高等学校科技计划项目(No.J12LL63)
【分类号】：R-332;R747.2
【正文快照】： 作者单位:(1.滨州医学院附属医院神经内科,山东滨州256600;2.滨州医学院附属医院神经内科一病区护理部,山东滨州256600)偏头痛是临床常见的原发性头痛,以搏动样疼痛、活动时加重及反复发作为主要临床表现,可导致失能,严重影响患者的生活和工作,全球发病率为12%[1],是一个重大

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