FSH和SCF对大鼠非梗阻性无精症模型生精功能影响的初步研究

发布时间：2018-01-01 20:06

本文关键词：FSH和SCF对大鼠非梗阻性无精症模型生精功能影响的初步研究　出处：《昆明医学院》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨环磷酰胺和白消安不同剂量和不同用药方式对大鼠生精功能的影响,寻求较好的制作大鼠NOA模型的方式。方法：160只SPF级成年雄性Wistar大鼠随机分为10组,每组16只,第1-3组分别予以白消安20mg.kg-1,15mg.kg-1,10mg.kg-1单次腹腔注射,第4-6组分别予以环磷酰胺200mg.kg-1,100mg.kg-1,75mg.kg-1单次腹腔注射,第7-9组分别以白消安40mg.kg-1,20mg.kg-1,环磷酰胺200mg.kg-1单次灌胃给药,第10组予以生理盐水2.Oml行单次腹腔注射。NOA模型成功的标准是用药至大鼠的一个生殖周期约38天,大鼠睾丸、附睾组织切片找不到精子和精子细胞即为NOA模型成功。比较模型成功组和生理盐水组,模型失败组与生理盐水组的大鼠体重,一侧睾丸、附睾的重量及各级生精细胞数目统计学差异,结合大鼠的死亡率,得出实用的成模方式。结果：用药后38天第1组大鼠存活2只,第2组存活8只,第3组存活15只,第4组存活3只,第5组存活10只,第6组存活15只,第7组存活1只,第8组存活2只,第9组存活2只,生理盐水组大鼠无死亡。用药后38天,存活大鼠睾丸及附睾组织切片结果提示第1,2,7,8组达到NOA模型的标准,其余各组大鼠NOA模型效果欠佳。通过对各组成模方式的比较,综合模型成功且死亡率最低者为白消安15mg.kg-1单次腹腔注射是实用的Wistar大鼠NOA模型制作方式。结论：白消安15mg.kg-1单次腹腔注射可成功制造Wistar大鼠NOA模型且死亡率较低。目的：评估FSH、SCF对Wistar大鼠NOA动物模型生精功能的影响。方法：利用白消安15mg. kg-1单次腹腔注射制作72只Wistar大鼠NOA动物模型,抽样检查模型结果成功后。将模型分为两组：治疗组以大鼠全身血FSH、SCF总量的5倍于大鼠后腿肌肉注射；对照组予以等量的生理盐水行大鼠后腿肌肉注射；一周给药两次。给药后19天、38天、57天分批取大鼠一侧睾丸、附睾行组织切片,一侧睾丸、附睾制作成一张切片。计算曲细精管垂直切面内的精原细胞、精母细胞、精子细胞及精子的数量和垂直切面的附睾管内精子数量,每张切片观察符合标准的25个曲细精管。t检验比较治疗组和对照组不同用药时间的结果,规定P0.01差异有统计学意义。结果：①治疗组和对照组用药19天后平均每个曲细精管的精原细胞数分别为58.00±15.70和30.90±13.52,P=0.000；精母细胞分别为47.44±15.52和12.65±8.08,P=0.000；精子细胞及精子数分别为12.42±8.11和0,P=0.005。实验组和对照组用药19天后平均每个附睾管内的精子数分别为0和0,P=0。②治疗组和对照组用药38天后平均每个曲细精管的精原细胞数分别为66.77±20.56和40.65±15.89,P=0.000；精母细胞分别为56.82±21.93和18.50±13.66,P=0.000；精子细胞及精子数分别为61.17±25.86和27.65±9.62,P=0.002。实验组和对照组用药38天后平均每个附睾管内的精子数分别为77.55±55.18和41.05±29.20,P=0.000。③治疗组和对照组用药57天后平均每个曲细精管的精原细胞数分别为78.63±24.28和47.95±18.12,P=0.000；精母细胞分别为68.53±21.54和29.60±15.13,P=0.000；精子细胞及精子数分别为102.83±35.31和45.15±20.96,P=0.000。治疗组和对照组用药19天后平均每个附睾管内的精子数分别为122.13±80.67和58.50±26.15,P=0.000。结论：肌注FSH和SCF能够促进Wistar大鼠NOA模型生精功能的恢复。
[Abstract]:Objective: To explore the effects of different doses of cyclophosphamide and Bai Xiao an on spermatogenesis in rats and to find a better way to make NOA model in rats.
Methods: 160 SPF adult male Wistar rats were randomly divided into 10 groups, 16 rats in each group, the 1-3 group were treated with busulfan 20mg.kg-1,15mg.kg-1,10mg.kg-1 single intraperitoneal injection, 4-6 group were given a single intraperitoneal injection of cyclophosphamide 200mg.kg-1100mg.kg-1,75mg.kg-1 and group 7-9 respectively with busulfan 40mg.kg-1,20mg.kg-1, cyclophosphamide 200mg.kg-1 single dose administration of Tenth 2.Oml normal saline group were given a single intraperitoneal injection of.NOA model for the standard of success is a reproductive cycle of medication to rats for about 38 days, the rat testis, epididymis tissue sections to find sperm and sperm cells is the NOA model successfully. Compared model group and saline group, failure model weight group and the saline group rats testicle, epididymis weight and all levels of spermatogenic cells in the number of statistically significant difference, with the mortality of rats, the mode into practical.
Results: after 38 days of first rats, 2, second groups of 8 rats survived, third groups of 15 rats survived, fourth groups of 3 rats survived, fifth groups of 10 rats survived, sixth groups of 15 rats survived, seventh groups of 1 rats survived, eighth groups of 2 rats survived, Ninth groups of 2 rats survived, saline group of rats died. 38 days after treatment, survival of rat testis and epididymis tissue results indicated that group 1,2,7,8 reached the standard NOA model, the poor the rats NOA model. Through the comparison of the mode, a comprehensive model of success and the lowest mortality for busulfan 15mg.kg-1 single intraperitoneal injection Wistar is a NOA rat model of practical production.
Conclusion: the single intraperitoneal injection of 15mg.kg-1 can make the NOA model of Wistar rats successfully and the mortality rate is low.
Objective: To evaluate the effect of FSH and SCF on the spermatogenic function of NOA animal model in Wistar rats.
Methods: using busulfan 15mg. kg-1 single intraperitoneal injection of 72 Wistar rats, NOA animal model, sampling inspection results after the success of model. The model is divided into two groups: treatment group in blood of FSH rats, SCF 5 times the total in rat hind leg muscle injection; the control group was given the same volume of saline for rat hind leg muscle injection; administered two times a week. For 19 days, 38 days after administration, the 57 batch of talent from rat testicle, epididymis side for tissue sections of testis, epididymis produced a slice. Calculation of the seminiferous tubules in the vertical section of spermatogonia, spermatocytes, the number and vertical section of the sperm cell and sperm of epididymis tube sperm, observe each slice 25 compliant seminiferous tubules.T test between the treatment group and control group in different time of medicine prescribed results, there were significant differences in P0.01.
Results: the treatment group and the control group of spermatogonial cell number after 19 days of treatment, the average of the seminiferous tubules were 58 + 15.70 and 30.90 + 13.52, P=0.000; spermatocytes were 47.44 + 15.52 and 12.65 + 8.08, P=0.000; sperm cells and sperm counts were 12.42 + 8.11 and 0, P= 0.005. the experimental group and the control group after 19 days of treatment the average sperm count in the epididymal duct were 0 and 0, P=0. in the treatment group and control group the number of spermatogonia after 38 days, the average of the seminiferous tubules were 66.77 + 20.56 and 40.65 + 15.89, P=0.000; spermatocytes were 56.82 and 21.93. 18.50 + 13.66, P=0.000; sperm cells and sperm counts were 61.17 + 25.86 and 27.65 + 9.62, P=0.002. experimental group and control group after 38 days of treatment the average sperm count in the epididymal duct were 77.55 + 55.18 and 41.05 + 29.20, P=0.000. of the treatment group and control group after 57 days of treatment The average number of spermatogonia in the seminiferous tubules were 78.63 + 24.28 and 47.95 + 18.12, P=0.000; spermatocytes were 68.53 + 21.54 and 29.60 + 15.13, P=0.000; sperm cells and sperm counts were 102.83 + 35.31 and 45.15 + 20.96, P=0.000. treatment group and control group after 19 days, the average the number of sperm in the epididymal duct were 122.13 + 80.67 and 58.50 + 26.15, P=0.000.
Conclusion: the intramuscular injection of FSH and SCF can promote the recovery of spermatogenesis in the NOA model of Wistar rats.

【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R698.2

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