强脉冲光对红毛癣菌的体外抑制作用

发布时间：2018-01-01 23:34

本文关键词：强脉冲光对红毛癣菌的体外抑制作用　出处：《南方医科大学学报》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨强脉冲光420 nm滤光片在体外对红色毛癣菌的抑制作用及其机制。方法实验所用红色毛癣菌落及菌悬液各分为处理组(强脉冲光照射)和对照组(未照射),在照射前、照射后第2天、第3天,拍摄各组菌落,使用Photoshop cx7软件以像素计算菌落的面积观察菌落生长曲线变化,以及在照射6 h后,通过噻唑蓝检测真菌菌悬液的活力;2',7'-二氯荧光黄双乙酸盐荧光探针检测各组真菌内活性氧变化,硫代巴比妥酸法检测各组真菌丙二醛含量改变。结果 IPL420 nm对红色毛癣菌具有损伤作用,其最适有效治疗参数为12 J/cm~2 12脉冲,处理后6 h后菌株活力下降约30%(P0.05),而处理后平板菌落停止生长,生长曲线趋于直线;同时IPL处理后真菌内活性氧水平升高(P0.05),伴随丙二醛含量上升(P0.05)。结论 IPL420 nm可在体外通过诱导红色毛癣菌内产生氧化应激,导致真菌损伤和死亡。
[Abstract]:Objective to investigate the inhibitory effect of 420nm strong pulse light filter on Trichophyton rubrum in vitro and its mechanism. Methods the colony and suspension of Trichophyton rubrum were divided into treatment group (irradiated by intense pulse light). And control group (no irradiation). Before, on the second and third day after irradiation, the colonies were photographed and the colony growth curve was observed by using Photoshop cx7 software to calculate the colony area in pixels. The activity of fungal suspension was detected by thiazolyl blue after irradiation for 6 h. The changes of reactive oxygen species (Ros) in fungi were detected by fluorescence probe of Dichlorofluorescein yellow diacetate. The content of malondialdehyde (MDA) was detected by thiobarbituric acid method in each group. Results IPL420 nm could damage Trichophyton rubrum. The most effective treatment parameter was 12 J / cm ~ (2) pulse. After 6 h treatment, the strain activity decreased about 30% P0.05, and the plate colony stopped growing after treatment. The growth curve tends to be straight; At the same time, the level of reactive oxygen species in fungi increased after IPL treatment (P0.05). Conclusion IPL420 nm can induce oxidative stress in Trichophyton rubrum and lead to fungal injury and death in vitro.
【作者单位】：南方医科大学附属顺德第一人民医院皮肤科;
【基金】：佛山市卫生和计生局医学科研课题(20170295) 佛山市十三五特色专科建设项目
【分类号】：R379
【正文快照】： 浅部真菌病是指限于皮肤最外层、甲板、毛发和黏膜的感染,主要包括皮肤癣菌病、浅表念珠菌病和花斑癣等[1-2]。红色毛癣菌是最常见的一种皮肤癣菌,可引起各种癣病,且病程持久、难治愈、易复发,在免疫系统受损的人群中尤为显著[3]。然而,由于各种因素,例如逐年来耐药率的上升、

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