邻苯二甲酸二乙基已酯（DEHP）及代谢产物邻苯二酸—单-2-乙基已酯（MEHP）对幼鼠精原细胞影响的实验研究

发布时间：2018-01-02 00:32

本文关键词：邻苯二甲酸二乙基已酯（DEHP）及代谢产物邻苯二酸—单-2-乙基已酯（MEHP）对幼鼠精原细胞影响的实验研究　出处：《遵义医学院》2011年硕士论文　论文类型：学位论文

【摘要】：目的：了解环境内分泌干扰因子邻苯二甲酸二乙基已酯(Ethylhexyl phthalate,DEHP)及代谢产物邻苯二酸-单-2-乙基己酯(single-ethylhexyl phthalate,MEHP)对幼鼠精原细胞有否损害。方法：生后2周龄的Wistar雄性幼鼠96只,随机分8组,每组12只。随机选择1组行生理盐水(0.9%NS 0.2ml·kg-1·d-1喂养3周)灌胃,作为正常对照组(NC组)；再选1组行环磷酰胺(CTX 100mg·kg-1·d-1,喂养1周)灌胃,作为阳性对照组(PC组)；余各组分别用DEHP、MEHP按低剂量(100mg·kg-1·d-1,喂养3周)、中剂量(200mg·kg-1·d-1,喂养2周)、高剂量(300mg·kg-1·d-1,喂养1周)灌胃制作动物模型。观察不同时期、不同剂量下睾丸精原细胞的变化,并应用TUNEL法检查精原细胞凋亡,免疫组化法检测睾丸组织中c-kit、PI3K的表达,放射免疫法检测血清性激素(卵泡刺激素FSH、黄体生成素LH以及睾酮T)水平。结果：(1)、睾丸精原细胞的变化：用药组精原细胞萎缩、发育落后,数量少、线粒体肿胀、空泡化等,但在用药的高剂量组短时间内损害与低剂量组长时间的损害一致； (2)、睾丸组织中精原细胞凋亡：与NC组比较,用药组精原细胞凋亡显著增多(P0.01),但在高剂量短时间喂养组与低剂量长时间喂养组间比较,差异无统计学意义(P0.05)； (3)、睾丸组织中c-ki、PI3K的表达：与NC组比较,用药组睾丸组织中c-kit及其下游信号分子PI3K的表达显著减少(P0.01),但高剂量短时间喂养组与低剂量长时间喂养组间比较,差异也无统计学意义(P0.05)； (4)、血清性激素水平：与NC组比较,用药组FSH、LH水平升高,IT水平下降(P0.05)；但用药的高剂量组与低剂量组间比较差异无统计学意义(P0.05)。结论：DEHP及代谢产物MEHP对幼鼠精原细胞有明显损害,推测可能对小儿的生精功能也有损害,小儿应避免过多的接触DEHP及代谢产物MEHP的物质。
[Abstract]:Objective: to understand the environmental endocrine disrupting factor, Ethylhexyl phthalate (DEHP two) and the metabolite of phthalate mono -2- ethylhexyl ester (single-ethylhexyl phthalate, MEHP), which can damage spermatogonia in young rats.
Methods: after 2 week old male Wistar rats 96 rats, were randomly divided into 8 groups, 12 rats in each group. 1 groups were randomly selected for saline (0.9%NS 0.2ml - kg-1 - D-1 for 3 weeks) by gavage, as normal control group (group NC); group CTX (cyclophosphamide 1 choose 100mg kg-1. D-1, for 1 weeks) by gavage, as positive control group (PC group); the other groups were treated with DEHP and MEHP at low dose (100mg - kg-1 - D-1, for 3 weeks), middle dose (200mg - kg-1 - D-1, for 2 weeks), high dose (300mg - kg-1 - D-1, feeding 1 weeks) animal model by gavage. Observation of different period, changes of different doses of spermatogonia, and the application of TUNEL method to examine the apoptosis of spermatogenic cells, c-kit immunohistochemistry was used to detect the expression of PI3K in testicular tissue, serum hormone radioimmunoassay (FSH FSH, LH LH T) and testosterone level.
Results: (1) the changes of testicular spermatogonial cells: the spermatogonial atrophy, the number of development was backward, the number of mitochondria was swollen and vacuolated, but in the high-dose group, the damage was consistent with the long time damage of low dose group.
(2) spermatogonia apoptosis in testicular tissue: compared with group NC, apoptosis in spermatogonia increased significantly (P0.01), but there was no significant difference between high-dose short time feeding group and low dose long time feeding group (P0.05).
(3) the expression of c-ki and PI3K in testicular tissue: compared with NC group, the expression of c-kit and its downstream signal molecules PI3K in the testicular tissue decreased significantly (P0.01), but there was no significant difference between the high-dose short time feeding group and the low dose long time feeding group (P0.05).
(4) serum sex hormone level: compared with group NC, the level of FSH and LH increased and IT level decreased (P0.05), but there was no significant difference between high dose group and low dose group (P0.05).
Conclusion: DEHP and its metabolite MEHP have obvious damage to spermatogonia in young rats. It is presumed that they may also damage the Spermatogenesis Function of children. Children should avoid excessive exposure to DEHP and metabolites MEHP.

【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329

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