马尔堡病毒GP蛋白高抗原性片段筛选及免疫性研究

发布时间：2018-01-02 05:34

本文关键词：马尔堡病毒GP蛋白高抗原性片段筛选及免疫性研究　出处：《军事医学》2017年06期 　论文类型：期刊论文

【摘要】：目的从马尔堡病毒GP蛋白上寻找与GP免疫原性相当的小片段用于疫苗设计,以克服全长蛋白缺陷。方法基于GP(aa:1~681)结构及功能分区,构建3个片段[GP1△(aa:25~239)、GPM(aa:250~520)、GP2△(aa:436~648)],经原核表达后免疫小鼠,不同时间检测血清特异抗体滴度,检测脾淋巴细胞增殖指数及测定细胞因子浓度。结果 ELISA检测结果显示,GP2△组与GP混合组(GP1△+GPM+GP2△)产生体液免疫能力相当,明显高于GP1△与GPM组(P0.05);Con A非特异刺激后,GP2△组脾淋巴细胞的SI值高于GP混合组(P0.05),GP混合组特异刺激后,GP2△组脾淋巴细胞的刺激指数(SI)值低于GP混合组(P0.05);细胞因子检测中,GP2△组的白细胞介素2(IL-2)及干扰素γ(IFN-γ)浓度均高于对照组,低于GP混合组(P0.05)。结论马尔堡病毒GP2△片段免疫小鼠能诱导与完整GP蛋白相当的体液免疫,同时也能诱导一定的细胞免疫,可用来替代完整GP用于疫苗设计。
[Abstract]:Objective to search for small fragments of Marburg virus GP protein equivalent to GP immunogenicity for vaccine design in order to overcome the defects of full-length protein. Construction of 3 fragments. [GP1: a: 25 / 239GPM A: 250520 / GP2 / 436 / 648], the mice were immunized with prokaryotic expression. Serum specific antibody titers, spleen lymphocyte proliferation index and cytokine concentration were detected at different times. The humoral immune ability of GP2 group and GP mixed group was significantly higher than that of GP1 and GPM group (P 0.05). The SI value of splenic lymphocytes in Con A non-specific stimulation group was higher than that in GP mixed group after specific stimulation. The stimulation index (SI) of splenic lymphocytes in GP2 group was lower than that in GP mixed group (P 0.05). The levels of IL-2 and IFN- 纬 in GP2 group were higher than those in control group. Conclusion GP2 fragment of Marburg virus can induce humoral immunity as well as cellular immunity of mice with intact GP protein. Can be used to replace the complete GP for vaccine design.
【作者单位】：军事医学科学院生物工程研究所;
【基金】：艾滋病和病毒性肝炎等重大传染病防治科技重大专项资助项目(2016ZX10004001)
【分类号】：R373
【正文快照】： 马尔堡病毒自1967年首次发现以来,在非洲多地已暴发数十次,造成数百人感染,死亡率80%[1,2]。马尔堡病毒感染后病情发展迅速,人体免疫系统及各脏器被病毒入侵,感染1周内即出现严重的出血热症状[3]。该病毒传染性强,可通过体液传染,目前无有效治疗药物以及预防用疫苗[4]。包膜

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