云南省瑞丽市2016年登革病毒包膜蛋白基因进化特征分析

发布时间：2018-01-02 14:27

本文关键词：云南省瑞丽市2016年登革病毒包膜蛋白基因进化特征分析　出处：《病毒学报》2017年06期 　论文类型：期刊论文

【摘要】：为阐明云南省德宏州瑞丽市2016年登革病毒(Dengue virus,DENV)流行株包膜蛋白(Envelope protein,E)基因进化特征及其血清型、基因型和流行病学特点。采集登革热患者急性期血清标本,用RT-PCR法检测DENV核酸,用C6/36细胞培养法分离病毒,并进行DENV C/PrM和E基因核苷酸序列测定,用MEGA6.0软件邻接法(Neighbor-Joining)进行E基因系统进化分析。结果从瑞丽市2016年登革热患者血清中分离到25株DENV(本地感染病例15株和缅甸输入性病例10株),并获得这25株病毒的E基因核苷酸序列,系统进化分析表明,14株为DENV血清型1型(DENV-1)的基因I型,2株为DENV-2亚洲基因I型,5株为DENV-3基因I型,4株为DENV-4基因I型。这些新分离株与2013~2015年瑞丽市(11DENV-1、4DENV-2和2DENV-4)和临沧市(2DENV-1)相同血清型DENV代表株的基因型和进化群相一致,而与西双版纳州2013年DENV-3基因II型和2015年DENV-2全球基因型不同。所有瑞丽市DENV流行株均与缅甸等东南亚国家流行株具有较近的亲缘关系。这4种血清型DENV广泛分布于中缅两侧边境地区。本研究证实,2016年我国瑞丽市存在着4种血清型DENV的流行,其中DENV-1、DENV-2和DENV-4与2013~2015年瑞丽市同血清型流行株的基因型及其进化群相同,DENV-3为2016年首次出现本地流行。所有血清型病毒的传播来源均为缅甸北部边境地区,应加强中缅两侧边境地区登革热联防联控工作。
[Abstract]:To elucidate the envelope protein Envelope protein of dengue virus (Dengue virus) in 2016 in Ruili City, Dehong Prefecture, Yunnan Province. The evolutionary characteristics, serotype, genotype and epidemiological characteristics of the gene were collected, and DENV nucleic acid was detected by RT-PCR method in the serum samples of patients with dengue fever in acute stage. The virus was isolated by C6 / 36 cell culture, and the nucleotide sequences of DENV C / PrM and E genes were determined. Using MEGA6.0 Software adjacency method (Neighbor-Jointing). The E gene phylogenetic analysis was carried out. Results 25 strains of DENVwere isolated from the sera of dengue fever patients in 2016 in Ruili City (15 cases of local infection and 10 strains of imported cases from Myanmar). The nucleotide sequences of E gene of the 25 strains were obtained. Phylogenetic analysis showed that 14 of the 25 strains were DENV serotype 1 (DENV-1) gene type I. 2 strains were DENV-2 Asian gene type I and 5 strains belonged to DENV-3 gene type I. The four strains were DENV-4 gene type I. these new isolates were compared with 11DENV-1 in Ruili city from 2013 to 2015. 4DENV-2 and 2DENV-4) the same serotypes of DENV were identical in genotype and phylogenetic group. But different from DENV-3 gene type II in 2013 and DENV-2 global genotype in 2015 in Xishuangbanna. All the DENV epidemic strains in Ruili city were similar to those in Burma and other Southeast Asian countries. These four serotypes of DENV are widely distributed in the border areas between China and Myanmar. In 2016, there were four serotypes of DENV in Ruili city, including DENV-1. The genotypes and phylogenetic groups of DENV-2 and DENV-4 were the same as those of the same serotype epidemic strains in Ruili city from 2003 to 2015. DENV-3 is the first local epidemic in 2016. All seroviruses are transmitted from the northern border areas of Myanmar. The joint control of dengue fever should be strengthened in the border areas between China and Myanmar.
【作者单位】：成都军区疾病预防控制中心;瑞丽市疾病预防控制中心;
【基金】：国家十三五重点研发计划项目(项目号:2016YFC1200100);题目:重要新发突发病原体发生与播散机制研究中国博士后基金项目(项目号:2016T91009);题目:云南边境重要虫媒传染病相关病毒可视化基因芯片研究~~
【分类号】：R373
【正文快照】： 登革热(Dengue fever)是由登革病毒(Denguevirus,DENV)1、2、3和4血清型(Serotype,DENV-1、2、3、4)引起的一种急性传染病,埃及伊蚊(Aedes aegypti)和白纹伊蚊(Aedes albopictus)是主要传播媒介[1-3]。登革热广泛分布于全球热带和亚热带地区,东南亚地区为本病地方性流行区[1-4

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