灵芝多糖硫酸酯对记忆障碍模型小鼠学习记忆能力的影响

发布时间：2018-01-02 19:09

本文关键词：灵芝多糖硫酸酯对记忆障碍模型小鼠学习记忆能力的影响　出处：《中国兽医杂志》2017年06期 　论文类型：期刊论文

【摘要】：为探讨灵芝多糖硫酸酯(GLPS)对D-半乳糖致衰小鼠学习记忆能力的影响,本试验对灵芝多糖(GLP)进行硫酸酯化修饰,将50只昆明系小鼠随机分为5组:空白对照组、模型对照组、阳性对照组(吡拉西坦片50 mg/kg体重·d)、GLP给药组(50 mg/kg体重·d)、GLPS给药组(50 mg/kg体重·d),建立D-半乳糖致衰小鼠模型。进行跳台试验,并检测小鼠脑组织SOD活性和MDA含量的变化;免疫印迹检测小鼠海马组织Bcl-2和Caspase-3的影响。结果发现,GLP和GLPS组均能够明显延长小鼠跳台潜伏期,减少5 min内出现的错误次数,且均可升高SOD活性、降低MDA含量,但GLPS组效果明显优于GLP组;免疫印迹结果表明,GLPS组能够明显降低Caspase-3的表达,增强Bcl-2蛋白的表达,而GLP组影响不明显。说明GLPS可以显著改善D-半乳糖致衰小鼠的学习记忆能力,其机制可能与增强Bcl-2的表达及抑制Caspase-3的表达,抑制海马组织凋亡相关。
[Abstract]:In order to investigate the effect of Ganoderma lucidum polysaccharide (GLPS) on learning and memory ability of mice induced by D-galactose, this experiment modified Ganoderma lucidum polysaccharide (GLP) with sulfate. Fifty Kunming mice were randomly divided into 5 groups: blank control group, model control group and positive control group (Piracetam tablet 50 mg/kg body weight 路d). The mice model of aging induced by D-galactose was established in the GLP administration group (50 mg/kg body weight 路daps). The changes of SOD activity and MDA content in brain tissue of mice were detected. The effects of Bcl-2 and Caspase-3 in hippocampus of mice were detected by Western blot. The results showed that both GLP and GLPS groups could significantly prolong the latency of jumping stage in mice. The number of errors occurred within 5 min was reduced, and the activity of SOD was increased and the content of MDA was decreased. However, the effect of GLPS group was better than that of GLP group. The results of Western blot showed that GLPS group could significantly reduce the expression of Caspase-3 and enhance the expression of Bcl-2 protein. But the effect of GLP group was not obvious, which indicated that GLPS could significantly improve the learning and memory ability of aging mice induced by D-galactose. The mechanism may be related to enhancing the expression of Bcl-2, inhibiting the expression of Caspase-3 and inhibiting the apoptosis of hippocampal tissue.
【作者单位】：吉林医药学院药学院;
【基金】：国家自然科学基金(21102055) 吉林市科技计划项目(20156428) 吉林省卫计委青年科研课题(2015Q041)
【分类号】：R285.5;R-332
【正文快照】： 灵芝多糖(Ganoderma lucidum polysaccharide,GLP),灵芝的主要活性成分之一,具有抗肿瘤、抗衰老等作用,但天然多糖存在很多劣势[1]。采取一定的方法对多糖结构进行修饰获得理想的活性多糖是解决该问题的有效途径。目前改变多糖结构效果较好的方法是硫酸酯化法,其修饰结果是将

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