内嗅皮质在不同方式应激反应中的作用研究

发布时间：2018-01-02 20:41

本文关键词：内嗅皮质在不同方式应激反应中的作用研究　出处：《浙江大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：探讨内嗅皮质在急性复合应激反应和急性心理应激反应中的作用,并比较不同应激源对下丘脑室旁核c-Fos表达的影响。材料和方法：1.实验动物：健康雄性Sprague-Dawley (SD)大鼠,体重280±20g,每笼5只于浙江大学实验动物中心常规饲养。2.实验试剂：(1)鹅膏氨酸、台盼蓝；(2)兔抗鼠多克隆c-Fos抗体；(3)即用型SABC试剂盒,DAB底物试剂盒,正常山羊血清。3.实验方法：所有实验动物在饲养一周后随机分为7组：正常对照组(N)、急性足底电击组(F)、生理盐水注射双侧内嗅皮质+急性足底电击组(FC)、鹅膏氨酸损毁双侧内嗅皮质+急性足底电击组(FS)、急性束缚应激组(R)、生理盐水注射双侧内嗅皮质+急性束缚应激组(RC)、鹅膏氨酸损毁双侧内嗅皮质+急性束缚应激组(RS)。用鹅膏氨酸化学损毁FS、RS组大鼠双侧内嗅皮质,术后2周,对F组、FC组和FS组建立不可逃避的急性足底电击应激模型,对R组、RC组和RS组建立急性束缚应激模型(N组大鼠不遭受应激),采用c-Fos免疫组织化学方法,观察急性足底电击1小时后及急性束缚应激1小时后下丘脑室旁核快反应基因c-Fos的表达情况,并比较急性足底电击应激和急性束缚应激对下丘脑室旁核c-Fos表达的影响。用SPSS16.0统计软件对实验数据进行统计分析。c-Fos实验数据采用非参数检验的Kruskal-Wallis H检验,以P0.05为判断差异显著性的标准。结果：(1)损毁大鼠双侧内嗅皮质能抑制不可逃避的急性足底电击应激反应时PVN c-Fos的表达；(2)再次验证损毁内嗅皮质能抑制急性束缚应激时PVN中c-Fos的表达；(3)急性足底电击应激和急性束缚应激诱导的PVN c-Fos的表达无显著差异。结论：(1)不可逃避的急性足底电击模型既有躯体应激成分也有心理应激成分,证明内嗅皮质参与急性复合性应激反应时HPA轴的功能调节。(2)束缚应激作为一种较好的心理应激模型,证明内嗅皮质参与急性心理应激反应时HPA轴的功能调节。结合内嗅皮质在复合应激反应中的作用,提示内嗅皮质至少参与厌恶性刺激的某些方面的信息处理过程,比如情绪部分。(3)不同应激源诱导的PVN c-Fos的表达可能与应激源的控制性有关；(4)综合本课题组以往的研究,我们得出内嗅皮质功能与生理应激和心理应激均关系密切,但具体的调节机理有待进一步研究。
[Abstract]:Objective: to investigate the role of endolfactory cortex in acute compound stress response and acute psychological stress response. The effects of different stress sources on the expression of c-Fos in hypothalamic paraventricular nucleus were compared. Materials and methods: 1. Experimental animal: healthy male Sprague-Dawley rats. The body weight was 280 卤20g, 5 rats per cage were fed in Zhejiang University Experimental Animal Center. (2) Rabbit anti-mouse polyclonal c-Fos antibody; Methods: all the experimental animals were randomly divided into 7 groups after one week of feeding: normal control group (control group). In the acute plantar shock group, normal saline was injected into the bilateral olfactory cortex of the acute plantar shock group (FCX), and the acute plantar shock group (FSs) was damaged by geese oinanine. Acute restraint stress group, saline injection of bilateral olfactory cortex acute restraint stress group (RC3). The acute restraint stress group of bilateral olfactory cortex was damaged by geese oinanine, and the bilateral olfactory cortex of FSNRS group was damaged by chemical damage of geese ointment ammonia acid, 2 weeks after operation, the rats in group F were divided into two groups. FC group and FS group to establish an unavoidable acute plantar shock stress model, R group RC group and RS group to establish an acute restraint stress model N group rats did not suffer from stress). The expression of c-Fos in hypothalamic paraventricular nucleus was observed by c-Fos immunohistochemical method after 1 hour of acute plantar shock and 1 hour of acute restraint stress. The effects of acute plantar shock stress and acute restraint stress on the expression of c-Fos in hypothalamic paraventricular nucleus were compared. The experimental data were statistically analyzed by SPSS16.0 software. Kruskal-Wallis with nonparametric test. H test. Results the bilateral olfactory cortex lesion could inhibit the expression of PVN c-Fos during the inescapable acute plantar shock stress in rats. (2) it was proved that the lesion of olfactory cortex could inhibit the expression of c-Fos in PVN under acute restraint stress. (3) there was no significant difference in the expression of PVN c-Fos between acute plantar shock stress and acute restraint stress. The inescapable acute plantar shock model has both somatic and psychological stress components. It is demonstrated that the entorhinal cortex participates in the regulation of the function of HPA axis in acute complex stress response. 2) restraint stress is a better psychological stress model. It is demonstrated that the entorhinal cortex is involved in the regulation of the HPA axis in acute psychological stress response and the role of the entorhinal cortex in the complex stress response. The results suggest that the entorhinal cortex is involved in at least some aspects of the information processing of aversion stimulation. For example, the expression of PVN c-Fos induced by different stressors may be related to the control of stressors. 4) based on the previous studies of our research group, we conclude that the function of the entorhinal cortex is closely related to physiological stress and psychological stress, but the specific regulation mechanism needs to be further studied.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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