PIK-75对体外细粒棘球蚴原头节的作用研究

发布时间：2018-01-03 02:23

本文关键词：PIK-75对体外细粒棘球蚴原头节的作用研究　出处：《中国病原生物学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨不同浓度PIK-75体外对细粒棘球蚴原头节生长作用及形态学的影响。方法将体外培养的细粒棘球蚴原头节分为空白对照组及PIK-75处理组(0.4、0.8、1.2、1.6及2.0μmol/L),处理后的原头蚴用经0.1%伊红染色,置于倒置显微镜下观察原头节的形态及活力,试验独立重复3次并绘制活力曲线图。用Caspase-3试剂盒检测作用24、48h后各浓度组原头节体内caspase-3酶活性;使用ELISA试剂盒测定不同浓度PIK-75作用后原头节体内抗氧化酶HO-1及NQO-1的活性变化。结果 PIK处理组原头节的形态结构发生变化,活力减弱,以2.0μmol/L时组原头节变化更明显,且在第6d时原头节均发生死亡。随着药物浓度的增高,PIK-75对细粒棘球蚴原头节生长的抑制作用越强。0.8μmol/L PIK-75处理组作用24h后原头节活性开始下降,在第3d约为67.4%,作用7d后活力为32.1%,空白对照组原头节形态及活力无明显变化。不同浓度PIK-75作用细粒棘球蚴原头节24、48h后caspase-3酶活性显著高于空白对照组(P0.05)。0.8μmol/L PIK-75作用原头节2d、5d后,HO-1活性分别为(704.265±5.082)pg/ml和(656.05±0.095)pg/ml,NQO-1活性分别为(2.236±0.018)ng/ml和(1.446±0.026ng/ml),与空白对照组比较显著降低(P0.05),且作用5d后的原头节酶活性显著低于作用2d组(P0.05)。结论 PIK-75能抑制体外细粒棘球蚴原头节的生长,降低抗氧化酶的活性,具体机制有待进一步研究。
[Abstract]:Objective to investigate the effects of different concentrations of PIK-75 on the growth and morphology of echinococcus granulosus in vitro. Methods the cultured Echinococcus granulosus was divided into blank control group and PIK-75 treated group. (. 0.4. The treated protocercariae were stained with 0.1% eosin and placed under inverted microscope to observe the morphology and activity of the protocephalic ganglia in the presence of 1.6 and 2.0 渭 mol 路L ~ (-1) L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). Caspase-3 kit was used to detect the activity of caspase-3 in the original cephalic ganglion of each concentration group after exposure for 24 h for 48 h. The activities of antioxidant enzymes HO-1 and NQO-1 were determined by using ELISA kit after different concentrations of PIK-75. The morphology and structure of the original head ganglia were changed in PIK treatment group. The activity decreased, the change of the original head ganglion was more obvious at 2.0 渭 mol/L, and the death of the original head ganglion occurred on the 6th day, with the increase of drug concentration. The stronger the inhibitory effect of PIK-75 on the growth of the protopectome of Echinococcus granulosus was. 0.8 渭 mol/L PIK-75 treated for 24 h, the activity of the original head began to decrease. On the 3rd day, it was about 67.4, and the activity was 32.1after 7 days. The morphology and activity of the original cephalus in the blank control group did not change obviously. The effect of PIK-75 at different concentrations on the original ganglia of Echinococcus granulosus was 24. After 48 h, the activity of caspase-3 enzyme was significantly higher than that of the control group (P0.05U. 0.8 渭 mol/L PIK-75). The activity of HO-1 was 704.265 卤5.082g / ml and 656.05 卤0.095pg / ml, respectively. The activity of NQO-1 was 2.236 卤0.018 ng / ml and 1.446 卤0.026 ng / ml, respectively, which was significantly lower than that of control group (P 0.05). After 5 days of treatment, the enzyme activity of protoganglia was significantly lower than that of P0.05 in 2 d group. Conclusion PIK-75 can inhibit the growth of procephalic ganglion of Echinococcus granulosus in vitro and decrease the activity of antioxidant enzyme. The specific mechanism needs further study.
【作者单位】：石河子大学医学院第一附属医院肝胆外科;石河子大学医学院组织胚胎学教研室;
【基金】：国家自然科学基金项目(No.U1303121,81360410)
【分类号】：R383.3
【正文快照】： ***囊型包虫病(Cystic echinococcosis,CE)由细粒棘球绦虫(Echinococcus granulosus,Eg)的幼虫阶段寄生于人体或宿主动物而引起的疾病,该病全球分布广泛,已成为严重的公共健康问题[1]。目前临床上治疗肝包虫病的药物有术中灌洗用的双氧水,20%的高渗生理盐水,以及术前、术后口

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