体积分数95%乙醇建立薄型子宫模型大鼠子宫内膜中波蛋白和血管内皮生长因子的变化

发布时间：2018-01-03 10:21

本文关键词：体积分数95%乙醇建立薄型子宫模型大鼠子宫内膜中波蛋白和血管内皮生长因子的变化　出处：《中国组织工程研究》2016年05期 　论文类型：期刊论文

【摘要】：背景:乙醇化学损伤法能够有效对大鼠子宫进行薄型子宫内膜建模,薄型子宫内膜增殖相关蛋白表达减少,有助于研究薄型子宫内膜相关发生机制,为临床治疗提供指导。目的:探索高浓度乙醇致薄型子宫后子宫内膜中波蛋白和血管内皮生长因子的表达变化。方法:SD雌鼠右侧子宫缓慢注入0.3 mL体积分数95%乙醇建立薄型子宫模型,左侧子宫注入等量生理盐水作为对照侧。结果与结论:与对照侧相比,大鼠损伤侧子宫内膜明显变薄,腺体数量减少,结构紊乱,波蛋白和血管内皮生长因子表达明显降低。说明体积分数95%乙醇损伤可有效建立大鼠薄型子宫内膜动物模型,且与增殖相关的蛋白在薄型子宫内膜中表达出现变化。
[Abstract]:Background: ethanol chemical damage method can be effectively used for thin endometrium model of rat uterus, the expression of thin endometrium proliferation related protein decreased, contribute to the study of thin endometrium related mechanism, and provide guidance for clinical treatment. Objective: To explore the expression of endometrial B protein and vascular endothelial growth factor induced by high concentrations of ethanol thin after the uterus. Methods: SD mice right uterine slow injection 0.3 mL 95% ethanol based thin uterine model, the left uterine injection of physiological saline as control group. Results and conclusion: compared with the control side, rats endometrium was significantly thinner, reducing the number of glands, structural disorder, wave protein and vascular endothelial the expression of growth factors that decrease obviously. The volume fraction of 95% ethanol injury can effectively establish a rat animal model of thin endometrium, and associated with the proliferation of protein in The expression of the thin endometrium changes in the thin endometrium.

【作者单位】：中国医科大学附属盛京医院妇产科辅助生殖中心;中国医科大学附属盛京医院实验研究中心;
【基金】：辽宁省科学技术计划项目(2012225014)~~
【分类号】：R-332;R711
【正文快照】： 0引言Introduction薄型子宫内膜是指子宫内膜厚度低于能够获得妊娠的阈厚度,即小于7 mm[1]。薄型子宫内膜可严重影响子宫的容受性,导致胚胎种植率明显降低[2],且患者还表现出月经过少、不孕等症状[3]。然而,导致薄型子宫内膜的原因尚不能完全确定。目前公认的危险因素有高龄、

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