人宫颈癌SiHa细胞荷瘤裸鼠模型的建立及鉴定

发布时间：2018-01-04 09:21

本文关键词：人宫颈癌SiHa细胞荷瘤裸鼠模型的建立及鉴定　出处：《温州医科大学学报》2016年02期 　论文类型：期刊论文

【摘要】：目的:建立人宫颈癌SiHa细胞裸小鼠体表荷瘤模型,并对其成瘤特性进行分析和鉴定。方法:将5×10~6/mL、1×10~7/mL及5×10~7/mL 3个浓度的SiHa细胞悬液分别在3组裸小鼠背部右腋近上肢皮下接种,建立人宫颈癌裸小鼠体表荷瘤模型,观察荷瘤裸鼠的成瘤率并测量肿瘤的大小,5周后取肿瘤组织经病理学方法观察其组织学特性,并采用PCR及免疫组织化学方法对肿瘤组织的HPV16E7 DNA表达进行检测。结果:3组裸小鼠接种SiHa细胞的成瘤率均为100%,5周后肿瘤大小分别达到(77.29±28.34)mm~3、(178.91±61.55)mm~3和(305.11±12.62)mm~3。3个浓度组间肿瘤体积大小差异有统计学意义(P0.001)。病理学大体观察可见SiHa肿瘤的生长均以局部浸润为主;组织切片观察新生肿瘤细胞特点呈现体积大、核大深染及易见核分裂相的病理性特征;SiHa新生肿瘤组织经PCR检测证实了HPV16E7 DNA阳性,并经免疫组织化学检测证实了HPV16E7蛋白表达阳性。结论:成功建立了SiHa细胞荷瘤裸鼠模型,为人宫颈癌肿瘤靶向治疗及生物学特性研究提供了理想的动物模型。
[Abstract]:Objective: to establish a nude mouse model of human cervical cancer SiHa cells and to analyze and identify the tumorigenic characteristics of nude mice. Methods: 5 脳 10 ~ (6) / mL of human cervical cancer cells were used. Three concentrations of SiHa cell suspension of 1 脳 10 ~ (7) / mL and 5 脳 10 ~ (7) / mL were inoculated subcutaneously in the right axillary proximal upper extremity of three groups of nude mice to establish the model of human cervical carcinoma nude mice. The tumorigenesis rate of nude mice bearing tumor was observed and the tumor size was measured 5 weeks later, the histological characteristics of tumor tissue were observed by pathological method. PCR and immunohistochemical methods were used to detect the expression of HPV16E7 DNA in tumor tissue. Results the tumorigenic rate of the nude mice inoculated with SiHa cells in the three groups was 100%. After 5 weeks, the tumor size reached 77.29 卤28.34 mm ~ (-3) respectively. There was significant difference in tumor volume size between 178.91 卤61.55mm3 and 305.11 卤12.62mm3 groups (P 0.001). Gross pathological observation showed that the growth of SiHa tumors was mainly local invasion. The histopathological features of neoplastic cells were large size, large nuclear staining and easy to see mitotic phase. HPV16E7 DNA was confirmed by PCR in the neoplasm of SiHa. The expression of HPV16E7 protein was confirmed by immunohistochemistry. Conclusion: the model of nude mice bearing tumor of SiHa cells was successfully established. Targeted therapy and biological characteristics of human cervical cancer provide an ideal animal model.
【作者单位】：温州医科大学分子病毒与免疫研究所微生物学与免疫学教研室;温州医科大学附属第一医院胃肠外科;
【基金】：国家自然科学基金资助项目(81176423)
【分类号】：R737.33;R-332
【正文快照】： 宫颈癌是全球最常见的妇科恶性肿瘤之一,其发病率在女性恶性肿瘤中仅次于乳腺癌排第二位,而我国宫颈癌患病率和病死率约占世界的28.8%,宫颈癌患病人数约占世界三分之一[1]。宫颈癌的发病与人乳头瘤病毒(human papilloma-virus,HPV)感染的相关性已经明确,且基于HPV L1蛋白的

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