G蛋白耦联型雌激素受体敲除大鼠的应激性高血压反应（英文）

发布时间：2018-01-04 14:24

本文关键词：G蛋白耦联型雌激素受体敲除大鼠的应激性高血压反应（英文）　出处：《生理学报》2017年05期 　论文类型：期刊论文

【摘要】：大量研究显示雌激素通过传统核受体ERα或ERβ以及新型膜受体——G蛋白耦联雌激素受体(G protein coupled estrogen receptor,Gper)对心血管系统起多方面保护作用。然而,文献报道的Gper基因敲除(Gper-knockout,Gper-KO)小鼠心血管系统表型差异很大。本研究旨在揭示Gper-KO对大鼠动脉血压和心率的影响。GperKO SD大鼠系采用CRISPR-Cas9基因编辑技术制备。我们对10周龄雄性Gper-KO大鼠(n=6)、12周龄雌性Gper-KO大鼠(n=6)以及同龄野生型(wild type,WT)大鼠(雌雄各6只),在清醒和束缚制动条件下进行无创检测尾动脉血压,连续检测8~9天,继而又在戊巴比妥麻醉下直接测量股动脉血压。WT雄性大鼠的尾动脉血压在第1至第4天略高于第5至第9天,表明在实验初期束缚应激引起的交感系统兴奋使大鼠血压升高,随着大鼠对束缚应激的逐步适应,其血压恢复至正常水平。雄性或雌性Gper-KO大鼠的动脉血压在实验初期都高于WT组(雄性大鼠:第1天至第5天;雌性大鼠:第1天至第3天),而随后几天的血压与WT组之间没有明显差异。雄性Gper-KO大鼠的心率高于雄性WT大鼠,雄性和雌性Gper-KO大鼠的体重增加值均较WT组大鼠减少。在麻醉状态下,Gper-KO组和WT组大鼠动脉血压无明显差异。以上结果表明,Gper-KO大鼠可能对应激性交感系统兴奋更为敏感,提示Gper在应激状态下的心血管功能调节中发挥重要作用。
[Abstract]:A large number of studies have shown that estrogen is coupled to estrogen receptors via the traditional nuclear receptor ER 伪 or ER 尾 and a novel membrane receptor-G protein. G protein coupled estrogen receptor. However, the Gper gene knockout reported in the literature is Gper-knockout. Gper-KOO). The purpose of this study was to reveal the effects of Gper-KO on arterial blood pressure and heart rate in rats. Sprague-Dawley (SD) rats were prepared by CRISPR-Cas9 gene editing technique. 6). 12-week-old female Gper-KO rats (n = 6) and wild type wild type WTT rats (male and female, n = 6). The blood pressure of caudal artery was measured by non-invasive method under the condition of awake and restraint braking, and the blood pressure of caudal artery was measured continuously for 8 days. Then under pentobarbital anesthesia, the arterial blood pressure of femoral artery. WT male rats was slightly higher than that of the 5th to 9th day on the 1st to 4th day. The results showed that the sympathetic system excited by restraint stress increased the blood pressure of rats at the beginning of the experiment and gradually adapted to restraint stress. The arterial blood pressure of male or female Gper-KO rats was higher than that of WT group at the beginning of the experiment (male rats: 1st to 5th day; Female rats: from day 1 to day 3, there was no significant difference between the blood pressure and WT group in the following days. The heart rate of male Gper-KO rats was higher than that of male WT rats. The weight gain of male and female Gper-KO rats was lower than that of WT group. There was no significant difference in arterial blood pressure between Gper-KO group and WT group under anesthesia. Gper-KO rats may be more sensitive to stress sympathetic system excitation, suggesting that Gper plays an important role in the regulation of cardiovascular function under stress.
【作者单位】：上海交通大学医学院同仁医院/虹桥国际医学研究院基础医学院生理学教研室;宁夏医科大学颅脑疾病重点实验室;
【基金】：supported by grants from the National Natural Science Foundation of China(No.31171066) the Sino-German Science Center(No.GZ919)
【分类号】：R331
【正文快照】： There is a significant gender-associated difference inthe incidence of cardiovascular diseases such as arterialhypertension and heart diseases.Menopause is accom-panied by slight but significant elevations in bloodpressure and higher risk of heart diseas

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