中枢神经元中SEL1L基因缺失对小鼠行为学的影响

发布时间：2018-01-04 16:22

本文关键词：中枢神经元中SEL1L基因缺失对小鼠行为学的影响　出处：《中国比较医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的探究内质网蛋白SEL1L在维持哺乳动物中枢神经系统正常生理活动中的作用。方法利用Cre/loxp技术,构建神经元(neuron)特异性SEL1L基因敲除小鼠(NKO),按照不同性别分为实验组(NKO)与野生型组(WT),每组10只。比较分析小鼠在出生后不同日龄的存活时间、体重及抓力、平衡协调性、运动、焦虑情绪等行为学指标等差异,对SEL1L在中枢神经系统中的生理功能做出判断。结果 NKO小鼠存活时间为(6±3)周;其体重在第3周、第5周、第8周分别为WT小鼠的54.64%、40.54%、38.57%;NKO雄性小鼠的抓力在第3、5、8周分别为WT雄性小鼠的44.24%、48.09%、49.04%,NKO雌性小鼠为WT雌性小鼠的39.39%、50.19%、49.69%;第3、5、8周的NKO雄性小鼠的平衡协调性反应出的运动时间分别为WT小鼠的26.92%、41.58%、37.48%,NKO雌性小鼠为WT小鼠的46.02%、47.67%、38.48%;第3、5、8周的NKO雄性小鼠在旷场实验中运动路程、进入中心区域时间分别为WT小鼠的(24.63%,9.57%)、(25.87%,11.63%)、(51.96%、9.97%),NKO雌性小鼠为WT小鼠的(35.62%,25.93%)、(42.75%,9.77%)、(34.77%,14.49%),且表现出明显的焦虑情绪。差异有显著性(P0.001)。结论 NKO小鼠在存活时间、体重、平衡协调性、运动、焦虑情绪等行为学指标等方面都明显差于野生型小鼠,说明SEL1L蛋白在维持中枢神经正常功能中是必须的。
[Abstract]:Objective to explore the expression of SEL1L in the endoplasmic reticulum maintain the normal physiological activity in the mammalian central nervous system. The method of using Cre/loxp technology to construct (neuron), neuron specific SEL1L knockout mice (NKO), according to the different gender divided into experimental group (NKO) and wild type (WT group), 10 rats in each group. The comparative analysis the survival time of mice at different ages after birth, weight and grip strength, balance, movement, difference of anxiety behavior index, make a judgment on the physiological function of SEL1L in the central nervous system. The results of NKO mice survival time was (6 + 3) weeks; its weight in third weeks. Fifth weeks, eighth weeks respectively for WT mice 54.64%, 40.54%, 38.57%; grasping the male NKO mice at 3,5,8 weeks respectively for WT male mice were 44.24%, 48.09%, 49.04%, NKO female mice of WT female mice of 39.39%, 50.19%, 49.69%; NKO male mice at 3,5,8 weeks. Balance motion time response of WT mice were 26.92%, 41.58%, 37.48%, NKO female mice WT mice of 46.02%, 47.67%, 38.48%; NKO male mice at 3,5,8 weeks of exercise in the open field test in the journey into the center area time were WT mice (24.63%, 9.57%, (25.87%), 11.63%), (51.96%, 9.97%), NKO female mice WT mice (35.62%, 25.93%), (42.75%, 9.77%), (34.77%, 14.49%), and showed obvious anxiety. There was a significant difference (P0.001). Conclusion NKO in mice survival time, weight, balance and coordination exercise, anxiety, behavioral indicators and other aspects of the obvious difference in wild-type mice, indicating that SEL1L protein is essential in maintaining the normal function of central nervous system.

【作者单位】：苏州大学实验动物中心;苏州大学剑桥基因组研究中心;
【基金】：苏州大学特聘教授启动经费(编号:Q421600114)
【分类号】：R-332;R749.16
【正文快照】： 随着人口老龄化加速,老年痴呆症(即阿尔茨海默病)已成为威胁生命健康的四大疾病之一[1]。据统计,在我国60岁以上患病人群中,患老年痴呆症的比例约为1.6%,且随着人口老龄化而日趋严重[2]。神经退行性疾病(neurodegenerative diseases)是一类以脑部神经元发生退行性病变为特征的

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