Aire对巨噬细胞极化影响的研究

发布时间：2018-01-05 00:09

本文关键词：Aire对巨噬细胞极化影响的研究　出处：《中国免疫学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:研究自身免疫调节因子(Aire)对巨噬细胞极化的影响。方法:分别用LPS、IL-4以及LPS联合免疫复合物刺激小鼠单核巨噬细胞系RAW264.7细胞、稳定表达GFP-Aire的RAW264.7细胞(A33-3)细胞和稳定表达GFP的RAW264.7细胞(C1-6),使其向M1(LPS)、M2a(IL-4)和M2b(LPS联合免疫复合物)型巨噬细胞极化。通过Real-time PCR检测各组细胞中M1型巨噬细胞特征分子IL-1α、i NOS和IL-6,M2a型特征分子Arg-1和M2b型特征分子IL-10的表达水平,研究Aire对各种类型巨噬细胞极化的影响。结果:LPS在0.5μg/ml浓度时,RAW264.7细胞中M1型巨噬细胞产物IL-1α、i NOS和IL-6基因表达量最高;而IL-4以及LPS联合免疫复合物的刺激作用有显著的剂量依赖性,都在浓度最高时RAW264.7细胞中Arg1(M2a)和IL-10(M2b)基因表达量最高。LPS刺激后,A33-3细胞中IL-1α和i NOS表达水平明显高于C1-6细胞,IL-6则相反;IL-4及LPS联合免疫复合物刺激后,A33-3细胞中Arg1和IL-10的表达水平明显低于C1-6细胞。结论:Aire可能促进巨噬细胞向M1极化,同时抑制其向M2a和M2b极化。
[Abstract]:Objective: To study the immune regulatory factor (Aire) effect on macrophage polarization. Methods: using LPS, IL-4 and LPS combined with immune complex stimulated murine macrophage cell line RAW264.7 cells stably expressing GFP-Aire RAW264.7 cells (A33-3 cells) and RAW264.7 cells stable table up to GFP (C1-6), make it to the M1 (LPS), M2a (IL-4) and M2b (LPS combined with immune complex type). Through molecular macrophage polarization type M1 cells were detected with Real-time PCR IL-1 I NOS macrophage characteristics of alpha, and IL-6, the expression level of M2 Arg-1 and M2 B a characteristic molecular features of molecular IL-10, effects of Aire on various types of macrophage polarization.. results: LPS at 0.5 g/ml concentration, M1 RAW264.7 cells and macrophages in product IL-1 alpha, NOS and I IL-6 gene expression was the highest; and the stimulation of IL-4 and LPS combined with immune complexes were dose dependent, All Arg1 in the highest concentration of RAW264.7 cells (M2a) and IL-10 (M2b) gene expression was highest in A33-3 cells after.LPS stimulation, the expression level of IL-1 alpha and I NOS was significantly higher than that of C1-6 cells, IL-6 was on the contrary; IL-4 and LPS combined with immune complexes after stimulation, the expression level of Arg1 and IL-10 in A33-3 cells significantly less than C1-6 cells. Conclusion: Aire may stimulate macrophages to M1 polarization, while inhibiting the M2a and M2b polarization.

【作者单位】：吉林大学基础医学院免疫学系;吉林大学基础医学院病原免疫细胞遗传学实验中心;
【基金】：国家自然科学基金项目(81373127) 吉林省科技厅项目(20160414038GH)资助
【分类号】：R392
【正文快照】： 1本文受国家自然科学基金项目(81373127)和吉林省科技厅项目(20160414038GH)资助。2吉林大学基础医学院病原免疫细胞遗传学实验中心,长春130021。自身免疫调节因子(Aire)是一种转录因子,人类Aire基因的突变将导致由自身免疫应答所介导的、以多器官损伤为特征的一种常染色体隐

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