环境胁迫下大蹼铃蟾抗内毒素活性肽分子特征及临床前期研究

发布时间：2018-01-05 20:25

本文关键词：环境胁迫下大蹼铃蟾抗内毒素活性肽分子特征及临床前期研究　出处：《昆明医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：感染是引起人类死亡的最主要原因之一,尽管目前针对细菌有许多药物可进行针对性的杀灭治疗,但是对于感染后内毒素引起的脓毒血症却缺乏有效的治疗方法。内毒素(endotoxin)是革兰氏阴性菌细胞壁外膜中的重要组成部分,内毒素一旦入血或大量释放将会引起一系列瀑布效应,导致各个器官功能受损、免疫力下降而导致病人死亡。目前针对内毒素引起的感染性休克临床上更多的采用糖皮质激素保护器官功能,缺乏更进一步的主动对抗措施。大蹼铃蟾(Bombina maxima)是我国的特有物种,由于其生存环境较为恶劣,容易受到细菌及紫外线等生物物理条件刺激,其皮肤产生的分泌物种类成分复杂,具有很高的研究价值。本课题利用大蹼铃蟾作为研究对象,通过细菌及其内毒素刺激,鉴定皮肤分泌物中诱导产生的具有中和对抗内毒素作用的成分并探讨其作用机理,蟾皮肤分泌物中持续出现的抗菌肽组分在宿主接触环境微生物早期时发挥作用,提示皮肤受到胁迫后通过分泌抗菌肽迅速控制病原微生物侵人。同时研究发现,后天免疫作为机体更为有效、多样的保护机制,需要先天免疫效应因子的激活。通过收集细菌胁迫后蟾皮肤分泌物进行电泳发现,蟾皮肤分泌物中含有KB因子,从而证实蟾皮肤抗菌肤的表达通路是由Toll样受体(TLRs)介导的,而TLRs在连接先天免疫与后天免疫的关键环节中发挥重要作用。
[Abstract]:Infection is one of the major causes of human death, although there are many drugs for bacteria to kill the treatment of sepsis caused by the infection, but after endotoxin is the lack of effective treatment. Endotoxin (endotoxin) is an important component of outer membrane in gram negative bacteria, endotoxin into the blood or once a large number of release will trigger a series of cascade effect, leading to organ dysfunction, decreased immunity and resulting in the death of the patient. The endotoxin induced septic shock clinically more use of glucocorticoids to protect organ function, the lack of further active countermeasures. Toad (Bombina maxima) is a species endemic to China and because of their living environment is very bad, vulnerable to bacterial stimulation and UV biophysical conditions, the type of skin secretions produced Complex components, has high research value. The subject of the use of Bombina maxima as the research object, through the bacteria and endotoxin stimulation induced identification in the skin secretion with neutralization against endotoxin components and investigate its mechanism, persistent frog skin secretions of the antimicrobial peptide components in the host environment early microbial contact when prompted by the skin play a role in stress by the secretion of antimicrobial peptide rapid control of pathogenic microorganism invasion. At the same time, the study found that as the body acquired immunity is more effective, and various protection mechanisms, activate innate immune effectors. By collecting bacterial stress after frog skin secretions were found containing KB electrophoresis, factor of toad skin secretion in order to confirm the expression of antibacterial peptides, pathway of toad skin is composed of Toll like receptor (TLRs) mediated, TLRs in innate immunity and acquired connection It plays an important role in the key link of immunization.

【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R3411

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