腹针对慢性阻塞性肺疾病呼吸肌疲劳模型大鼠作用研究

发布时间：2018-01-07 03:05

本文关键词：腹针对慢性阻塞性肺疾病呼吸肌疲劳模型大鼠作用研究　出处：《中国中医急症》2016年01期 　论文类型：期刊论文

【摘要】：目的通过腹针治疗慢性阻塞性肺疾病(COPD)呼吸肌疲劳的动物实验研究,明确腹针治疗COPD呼吸肌疲劳的治疗作用,为进一步推广该诊疗技术提供动物实验证据。方法 SD大鼠30只随机数字表随机分为对照组、模型组、腹针组3组,每组10只。烟熏联合气道内注射脂多糖法建立COPD大鼠模型。连续治疗30 d后测定COPD大鼠血清和膈肌组织中的肿瘤坏死因子-α(TNF-α)、线粒体中还原型尼克酰胺腺嘌呤二核苷酸氧化酶(NOX)、琥珀酸脱氢酶(SDH)、细胞色素氧化酶(CCO)的活性。观察对照组、模型组、腹针组大鼠肺组织病理形态学的改变。结果模型组大鼠精神不振,活动及食量明显减少、眯眼、扎堆、反应迟钝、食少、体质量减轻、皮毛稀疏、灰黄无光泽有严重脱落、咳喘、呼吸道分泌物增加;正常组大鼠饮食正常、精神好,毛发光泽,反应灵敏,体质量正常。模型组大鼠体质量低于对照组(P0.05),针刺组大鼠体质量均高于模型组(P0.05)。模型组TNF-α含量高于空白对照组(均P0.05),腹针组TNF-α含量均低于模型组(均P0.05)。与对照组比较,模型组大鼠膈肌NOX、SDH、CCO组织线粒体中活性降低(均P0.05);与模型组比较,腹针组NOX、SDH、CCO活性均升高(均P0.05)。大鼠肺组织病理形态学观察,模型组支气管黏膜上皮变性、坏死、脱落,支气管壁出现炎性充血、水肿,有大量的炎性细胞浸润,支气管内有大量粘液性炎性渗出物。腹针组炎性充血、水肿,炎性细胞浸润显著减轻。结论腹针可能是通过减少TNF-α炎性介质释放,提升COPD模型大鼠膈肌线粒体NOX、SDH、CCO活性,从而减轻COPD病理表现,进一步实现改善COPD呼吸肌疲劳、延缓COPD的进程的功能。
[Abstract]:Objective to determine the therapeutic effect of abdominal acupuncture on respiratory muscle fatigue of chronic obstructive pulmonary disease (COPD) by using abdominal acupuncture in treating chronic obstructive pulmonary disease (COPD) respiratory muscle fatigue. Methods Thirty SD rats were randomly divided into three groups: control group, model group and abdominal acupuncture group. COPD rat model was established by fumigation combined with intraairway injection of lipopolysaccharide (LPS). Tumor necrosis factor- 伪 (TNF- 伪) in serum and diaphragm of COPD rats were measured after 30 days of continuous treatment. TNF- 伪). The activity of reductive nicotinamide adenine dinucleotide oxidase (NOXX), succinate dehydrogenase (SDH) and cytochrome oxidase (CCO) in mitochondria were observed. Results the rats in the model group showed mental retardation, decreased activity and food intake, squinting, piling up, slow response, little food, reduced body mass and sparse fur. Grey yellow luster has serious shedding, cough and asthma, respiratory tract secretion increased; The normal diet, good spirit, glossy hair, sensitive reaction, normal body mass of the model group rats were lower than that of the control group (P0.05). The body weight of the acupuncture group was higher than that of the model group (P 0.05), and the TNF- 伪 content of the model group was higher than that of the blank control group (all P 0.05). The content of TNF- 伪 in abdominal acupuncture group was lower than that in model group (all P 0.05). Compared with the control group, the activity of mitochondria in the model group was lower than that in the control group (all P 0.05). Compared with the model group, the CCO activity in the abdominal acupuncture group was higher than that in the control group (all P 0.05). The pathological morphology of lung tissue in the model group showed that the bronchial mucosal epithelium in the model group was denatured, necrotized and shedded. Bronchial wall appeared inflammatory hyperemia, edema, a large number of inflammatory cell infiltration, a large number of mucinous inflammatory exudates in the bronchus. Abdominal needle group inflammatory hyperemia, edema. Conclusion abdominal acupuncture may increase the activity of NOX- 伪 SDH CCO in the diaphragm of COPD rats by reducing the release of TNF- 伪 inflammatory mediators. Thus, the pathological manifestations of COPD were alleviated, and the function of improving respiratory muscle fatigue in COPD and delaying the progression of COPD was further realized.
【作者单位】：山西省中医药研究院;
【基金】：山西省科技厅青年课题(2013021037-2)
【分类号】：R245;R-332
【正文快照】： 慢性阻塞性肺疾病(COPD)严重影响患者的劳动能力和生活质量。预计中国在未来的半个世纪将会每年有150万人死于COPD,到2020年COPD将成为全球第5位影响生存时间和生活质量的疾病[1]。呼吸肌疲劳在COPD的发展过程中十分常见,呼吸泵功能衰竭是患者呼吸衰竭的重要原因,也是COPD患者

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