重复经颅磁刺激对抑郁模型大鼠行为学及海马区糖皮质激素受体表达的影响

发布时间：2018-01-07 05:12

本文关键词：重复经颅磁刺激对抑郁模型大鼠行为学及海马区糖皮质激素受体表达的影响　出处：《中国神经精神疾病杂志》2016年07期 　论文类型：期刊论文

【摘要】：目的观察重复经颅磁刺激(repetitive transcranial magnetic stimulation,r TMS)对慢性应激抑郁模型大鼠的抗抑郁作用及对海马区糖皮质激素受体(glucocorticoid receptor,GR)表达的影响,探讨r TMS抗抑郁作用的可能机制。方法 75只健康成年雄性大鼠随机分为造模组(60只)和空白对照组(15只),造模组采用孤养联合慢性温和不可预见应激(chronic unpredictability stimulus,CUMS)方法制备抑郁大鼠模型,为期3周,筛选造模成功的大鼠45只随机分为r TMS组、伪r TMS组和抑郁对照组,每组15只,r TMS组和伪r TMS组分别接受10 Hz的r TMS刺激和伪刺激干预3周,抑郁对照组和空白对照组不给予干预。分别于造模前、造模后、r TMS干预后进行体重测量、蔗糖水消耗实验和强迫游泳实验评估,r TMS干预后检测大鼠海马区GR蛋白和海马GR m RNA表达水平。结果造模后,r TMS组、伪r TMS组和抑郁对照组大鼠蔗糖水消耗量较空白对照组下降,强迫游泳不动时间增加(P0.01)。r TMS干预后,r TMS组体重增长率、蔗糖水消耗量与伪r TMS组和抑郁对照组相比均较高(P0.01),强迫游泳不动时间较短(P0.01)。伪r TMS组及抑郁对照组海马区GR蛋白及其m RNA表达水平与r TMS组和空白对照组相比均较低(P0.05)。结论 r TMS能够改善CUMS抑郁模型大鼠的抑郁样行为,可能与上调海马区GR表达有关。
[Abstract]:Objective to observe repeated transcranial transcranial magnetic stimulation. The antidepressant effect and the expression of glucocorticoid receptor GRG of glucocorticoid receptor in hippocampus of rats with chronic stress depression were studied. Methods 75 healthy adult male rats were randomly divided into model group (n = 60) and blank control group (n = 15). The depressive rat model was established by using the method of solitary nourishment combined with chronic mild and unpredictable stress (chronic unpredictability stimulation CUMS) in the model group. For 3 weeks, 45 rats were randomly divided into r TMS group, pseudo r TMS group and depression control group with 15 rats in each group. R TMS group and pseudo r TMS group received 10 Hz r TMS stimulation and pseudo stimulation for 3 weeks, while depression control group and blank control group did not. R TMS intervention was used to measure body weight, sucrose water consumption test and forced swimming test. After the intervention of r TMS, the expression of gr protein and gr m RNA in hippocampus of rats were detected. The consumption of sucrose water in the pseudo r TMS group and the depression control group was lower than that in the blank control group, and the immobility time of forced swimming increased the body weight growth rate of the r TMS group after the intervention of P0. 01 TMS. R TMS. The sucrose water consumption was higher than that in the pseudo r TMS group and the depression control group (P 0.01). The expression levels of gr protein and m RNA in hippocampus in pseudo r TMS group and depression control group were lower than those in r TMS group and blank control group (P 0. 01). Conclusion r TMS can improve the depressive behavior of CUMS model rats. It may be related to the up-regulation of gr expression in hippocampus.
【作者单位】：新乡医学院第二附属医院;
【基金】：河南省高等学校重点科研项目(编号:16A320044) 新乡市科技攻关计划项目(编号:ZG15019)
【分类号】：R749.4;R-332
【正文快照】： 近年来,大量研究表明抑郁症患者大脑诸多物不能预料刺激的发生,共21 d。空白对照组正区域糖皮质激素受体(glucocorticoid receptor,GR)常饲养,4只/笼。表达有所减少[1],在与情绪、认知及应激调节等均1.2分组与处理经行为学评估从造模组中筛选造有重要关系的海马组织中,角回3(c

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