不同时间点不同应激方法致大鼠焦虑样行为的研究

发布时间：2018-01-07 11:09

本文关键词：不同时间点不同应激方法致大鼠焦虑样行为的研究　出处：《中国比较医学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的从不同时间点比较几种不同的应激方法建立焦虑大鼠模型的生物学特点,为寻找最合适的造模方法提供实验依据。方法 70只大鼠随机分为正常组、空瓶应激组、慢性情绪应激(chronic emotional stress,CES)组、束缚应激3 h组、束缚应激6 h组,分别进行造模,于实验第7 d、14 d、21 d分别采用高架十字迷宫和场景恐惧测试测定大鼠的焦虑样行为,旷场测试考察大鼠的焦虑或抑郁样行为,强迫游泳实验测定大鼠的抑郁样行为,采用Elisa试剂盒检测大鼠海马组织中5-HT、DA含量。结果焦虑样行为学测试结果表明,第14天起,空瓶应激组、CES组、束缚应激6 h组大鼠开始产生焦虑样行为,此后焦虑样行为愈发明显,强迫游泳实验结果表明,束缚6 h组大鼠的不动时间于第7 d起即显著增加(P0.05)。同时,与正常大鼠比较,空瓶应激组和CES组大鼠海马5-HT、DA含量于第14天开始显著上升(P0.05或P0.01)。结论在上述几种焦虑模型中,束缚应激3 h焦虑样行为不明显;束缚应激6 h可能由于应激时间过长,表现出抑郁样行为。空瓶应激和CES能成功建立焦虑大鼠模型,且其焦虑行为表征有一定差异,可根据不同的实验目的选择对应的模型方法。
[Abstract]:The biological characteristics of stress from different time points to compare different anxiety to establish rat model, to find the most suitable model to provide experimental basis. Methods 70 rats were randomly divided into normal group, empty bottle stress group, chronic emotional stress (chronic emotional, stress, CES) group, 3 h restraint stress 6 h group, restraint stress group, respectively. The model was made on the seventh D, 14 d and 21 d respectively with anxiety like behavior and elevated plus maze test were measured at the scene of fear, open field tests in rats with anxiety or depression like behavior, depression like behavior in rats by forced swimming experiment using 5-HT, Elisa kit, tissue of rat hippocampus. The detection of DA content in anxiety like behavior test results show that, the fourteenth day, the empty bottle stress group, CES group, 6 h restraint stress rats began to produce anxiety like behavior, anxiety like behavior after the more obvious Forced swimming, the experimental results show that the immobility time bound 6 h rats in seventh D increased significantly (P0.05). At the same time, compared with the normal rats, the empty bottle stress group and CES in hippocampus of rats in 5-HT group, the content of DA on the fourteenth day increased significantly (P0.05 or P0.01). In conclusion the several kinds of anxiety model, restraint stress 3 h anxiety like behavior is not obvious; restraint stress may be due to stress 6 h long time, showed depression like behavior. Empty bottle stress and CES can successfully establish rat models of anxiety, and the anxiety behavior is different, according to different objectives selection model the corresponding.

【作者单位】：湖南中医药大学湖南省中药粉体与创新药物省部共建国家重点实验室培育基地;湖南中医药大学第一附属医院;
【基金】：湖南省科技厅重点项目(2015DK3003) 湖南省研究生创新课题(CX2016B371;2016CX14)
【分类号】：R749.72;R-332
【正文快照】： was becoming increasingly apparent.Forced swimming test results showed that immobility time in 6 h restraint rats wassignificantly increased in the first 7 d(P0.05).Meanwhile,compared with control group,hippocampal 5-HT,DAcontents in empty bottle stress

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