pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响

发布时间：2018-01-07 16:14

本文关键词：pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响　出处：《首都医科大学学报》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨L-精氨酸(L-arginine)不同给药方法诱导小鼠急性胰腺炎模型的效果。方法 L-精氨酸溶液以不同pH值、不同剂量诱导小鼠急性胰腺炎模型,造模后2、4、12、24 h统计小鼠病死率,采集小鼠血浆以淀粉-碘比色法检测淀粉酶活性,取胰腺及肺组织行石蜡切片HE染色观察组织损伤严重程度。结果 pH值为11高剂量组小鼠2 h病死率100%,其余各组各时间点小鼠均存活。3个模型组与对照组相比血浆淀粉酶活性、胰腺组织病理评分均升高,差异有统计学意义(P0.05)。模型组之间比较,pH值为7高剂量组胰腺及肺组织损伤最重。结论 L-精氨酸溶液的pH值及给药剂量对胰腺损伤程度有不同程度的影响,20%L-精氨酸溶液pH值为7按4 g/kg的剂量间隔1 h连续2次腹腔注射诱导的小鼠急性胰腺炎模型组织损伤重,且小鼠存活率高。
[Abstract]:Objective to investigate the effect of different administration of L-arginine on acute pancreatitis in mice. The acute pancreatitis model was induced in mice with different doses. The mortality of mice was calculated at 24 hours after the establishment of the model, and the amylase activity was measured by amylum-iodine colorimetry in plasma of mice. The severity of tissue injury was observed by HE staining in paraffin sections of pancreas and lung. Results the death rate of mice in high dose group was 100% in 2 hours. Compared with the control group, the plasma amylase activity and pancreatic histopathological score of the three model groups were higher than those of the control group, and the difference was statistically significant (P 0.05). Conclusion the pH value and dosage of L-arginine solution have different effects on the degree of pancreatic injury. The mice model of acute pancreatitis induced by intraperitoneal injection of 20L- arginine solution with pH value of 7 at a dose interval of 4 g / kg for 1 h was seriously damaged, and the survival rate of mice was high.
【作者单位】：首都医科大学附属北京安贞医院消化内科;首都医科大学附属北京安贞医院心肺血管疾病研究所;首都医科大学附属北京安贞医院病理科;
【基金】：国家自然科学基金(81470889)~~
【分类号】：R-332;R576
【正文快照】： 急性胰腺炎是临床常见病,急性重症胰腺炎病情进展快,病死率高[1]。动物模型是研究其病理机制、治疗疗效的重要途径。小鼠急性胰腺炎模型的建立方法很多,药物腹腔注射的方法具有操作简单、降低感染风险、对内环境影响小等优势,应用L-精氨酸腹腔注射是诱导小鼠急性胰腺炎的重要

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