巴马小型猪85%肝切除术后急性肝功能衰竭模型的建立

发布时间：2018-01-07 21:38

本文关键词：巴马小型猪85%肝切除术后急性肝功能衰竭模型的建立　出处：《中国实验动物学报》2017年02期 　论文类型：期刊论文

【摘要】：目的探索不同比例肝切除体积对巴马小型猪急性肝功能衰竭的影响,为建立合适的小型猪大部分肝切除后急性肝功能衰竭模型提供合适的方法。方法分别行75%、85%及95%肝切除,CT检查残留肝并记录存活情况,术前、术后1、3、5 d和术后1、2、3周定期抽血检测肝功能,获取肝组织HE染色,检查肝病理情况。结果75%、85%及95%肝切除小型猪平均存活时间为(19.0±5.6)d,(17.3±5.5)d,(1.3±1.5)d,不同肝切除比例的巴马小型猪病理学评分分别为(5.67±0.52)、(8.17±0.82)、(8.50±0.71)。随着肝切除比例增加,肝功能衰竭发病率升高。85%肝切除可引起谷丙转氨酶、谷草转氨酶、碱性磷酸酶、乳酸脱氢酶、总胆汁酸水平显著增高。结论85%体积肝切除可造成典型的小型猪急性肝功能衰竭模型。
[Abstract]:Objective to explore the effect of different proportion of hepatectomy volume on acute hepatic failure in Bama miniature pigs. Methods 75% and 95%% hepatectomy were performed to examine the residual liver and to record the survival of the residual liver. Before operation, 1 day after operation, 3 days after operation and 1 week and 2 weeks after operation, the liver function was measured regularly, liver tissue HE staining was obtained, and liver pathology was examined. Results 75%. The mean survival time of 85% and 95% hepatectomy mini-pigs was 19.0 卤5.6g / d, 17.3 卤5.5g / d and 1.3 卤1.5d / d respectively. The pathological scores of Bama miniature pigs with different hepatectomy ratios were 5.67 卤0.52mb, 8.17 卤0.82U, 8.50 卤0.71g, respectively, with the proportion of hepatectomy increasing. The incidence of liver failure increased by .85% hepatectomy can cause alanine aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase. Conclusion 85% volume hepatectomy is a typical model of acute hepatic failure in miniature pigs.
【作者单位】：南京大学医学院附属鼓楼医院肝胆外科;南京医科大学;
【基金】：国家自然科学基金(编号:81500478)
【分类号】：R575.3;R-332
【正文快照】： (1.Department of Hepatobiliary Surgery,Drum Tower Hospital Affiliated to Nanjing University Medical School,Nanjing 210008,China;2.Nanjing Medical University,Nanjing 211166)Corresponding author:DING Yi-tao,E-mail:kwh2003@263.net急性肝衰竭(acute liver fail

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